Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   NG824_RS15450 Genome accession   NZ_CP099534
Coordinates   3590313..3590723 (-) Length   136 a.a.
NCBI ID   WP_267092677.1    Uniprot ID   A0AA46SPC1
Organism   Xanthomonas sacchari strain JR3-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3585313..3595723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG824_RS15440 (NG824_15425) pilR 3586867..3588255 (+) 1389 WP_043095008.1 sigma-54 dependent transcriptional regulator Regulator
  NG824_RS15445 (NG824_15430) pilB 3588486..3590216 (-) 1731 WP_267092676.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NG824_RS15450 (NG824_15435) pilA/pilAI 3590313..3590723 (-) 411 WP_267092677.1 pilin Machinery gene
  NG824_RS15455 (NG824_15440) pilC 3591090..3592349 (+) 1260 WP_267092678.1 type II secretion system F family protein Machinery gene
  NG824_RS15460 (NG824_15445) - 3592356..3593219 (+) 864 WP_267092679.1 A24 family peptidase -
  NG824_RS15465 (NG824_15450) coaE 3593233..3593862 (+) 630 WP_267092680.1 dephospho-CoA kinase -
  NG824_RS15470 (NG824_15455) - 3593972..3595306 (-) 1335 WP_148828478.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14137.57 Da        Isoelectric Point: 8.8584

>NTDB_id=700480 NG824_RS15450 WP_267092677.1 3590313..3590723(-) (pilA/pilAI) [Xanthomonas sacchari strain JR3-14]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQVTAALAEINPGKAQYEVALNEGKTTITGIGDLGLKSPTNRCT
ITPITALSATGTIVCTMKGNTQVNTKTITLTRATDGSWSCATTVLAKHAPAGCPGV

Nucleotide


Download         Length: 411 bp        

>NTDB_id=700480 NG824_RS15450 WP_267092677.1 3590313..3590723(-) (pilA/pilAI) [Xanthomonas sacchari strain JR3-14]
ATGAAGAAGCAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCGATCATTGCCATCCTGGCCGCTATCGCGCT
GCCGATGTATCAGGACTATGTTGCCAAGTCGCAGGTCACCGCTGCGTTGGCCGAGATCAATCCTGGTAAGGCTCAGTATG
AAGTTGCTTTGAACGAGGGCAAGACCACCATCACCGGCATTGGCGATCTTGGTTTGAAGTCTCCGACGAACCGTTGCACT
ATCACTCCGATCACGGCTCTGAGTGCGACTGGTACCATCGTCTGCACGATGAAGGGCAATACGCAGGTCAACACCAAGAC
CATCACTTTGACGCGCGCGACCGATGGTTCTTGGAGTTGCGCAACCACCGTGCTTGCCAAGCATGCGCCTGCCGGTTGCC
CTGGCGTCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

56.296

99.265

0.559
  pilA Acinetobacter baumannii strain A118

50.365

100

0.507
  pilA Pseudomonas aeruginosa PAK

44.595

100

0.485
  pilA/pilAII Pseudomonas stutzeri DSM 10701

48.507

98.529

0.478
  pilA Vibrio cholerae C6706

38.621

100

0.412
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.621

100

0.412
  pilA Vibrio cholerae strain A1552

38.621

100

0.412
  pilA2 Legionella pneumophila str. Paris

41.481

99.265

0.412
  pilA2 Legionella pneumophila strain ERS1305867

41.481

99.265

0.412
  comP Acinetobacter baylyi ADP1

35.57

100

0.39
  pilE Neisseria gonorrhoeae MS11

40

95.588

0.382