Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NG824_RS15445 Genome accession   NZ_CP099534
Coordinates   3588486..3590216 (-) Length   576 a.a.
NCBI ID   WP_267092676.1    Uniprot ID   A0AA46SSE1
Organism   Xanthomonas sacchari strain JR3-14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3583486..3595216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG824_RS15430 (NG824_15415) sucC 3583610..3584779 (-) 1170 WP_010342227.1 ADP-forming succinate--CoA ligase subunit beta -
  NG824_RS15435 (NG824_15420) - 3585171..3586784 (+) 1614 WP_267092675.1 ATP-binding protein -
  NG824_RS15440 (NG824_15425) pilR 3586867..3588255 (+) 1389 WP_043095008.1 sigma-54 dependent transcriptional regulator Regulator
  NG824_RS15445 (NG824_15430) pilB 3588486..3590216 (-) 1731 WP_267092676.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NG824_RS15450 (NG824_15435) pilA/pilAI 3590313..3590723 (-) 411 WP_267092677.1 pilin Machinery gene
  NG824_RS15455 (NG824_15440) pilC 3591090..3592349 (+) 1260 WP_267092678.1 type II secretion system F family protein Machinery gene
  NG824_RS15460 (NG824_15445) - 3592356..3593219 (+) 864 WP_267092679.1 A24 family peptidase -
  NG824_RS15465 (NG824_15450) coaE 3593233..3593862 (+) 630 WP_267092680.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62457.68 Da        Isoelectric Point: 5.6425

>NTDB_id=700479 NG824_RS15445 WP_267092676.1 3588486..3590216(-) (pilB) [Xanthomonas sacchari strain JR3-14]
MTGVSANLVGITGIARRLVQDGALDEAAARAAMAQAGEAKVPLPQWFSEKKLVTAAQLAAANAVEFGMPLMDVSVFDASQ
NAMKLVSEELLQKYQVLPLFKRGGRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDSLGSALGD
DEGMDNLDVGGGDEDMGSGGDTGIDAKGEDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVQLPEK
ALLAEGFTEAEIAAGVQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQKIGVKDLRQSALLKA
RNGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=700479 NG824_RS15445 WP_267092676.1 3588486..3590216(-) (pilB) [Xanthomonas sacchari strain JR3-14]
ATGACGGGTGTCTCAGCCAACCTTGTCGGCATCACCGGGATCGCCCGCCGCCTTGTGCAGGACGGCGCGCTGGACGAAGC
CGCGGCGCGGGCCGCGATGGCCCAGGCGGGCGAGGCCAAGGTGCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGGTCA
CCGCCGCGCAGCTGGCCGCCGCCAATGCGGTGGAGTTCGGCATGCCGTTGATGGATGTCTCGGTCTTCGATGCCAGCCAG
AACGCGATGAAGCTGGTCAGCGAGGAGTTGCTGCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCGGCCGCCTGTT
CGTCGGCATCAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGACGGTGGAGCCGATCC
TGGTCGACGAGGACCAGATCCGCCGGACCCTGGAGCAGTGGCAGGCCAGCAACGATTCCCTCGGCAGCGCGCTGGGCGAC
GACGAGGGCATGGACAACCTCGATGTCGGCGGCGGCGACGAGGACATGGGCAGCGGTGGCGACACCGGCATCGATGCCAA
GGGGGAGGACACCCCGGTCGTCAAGTTCGTGAACAAGGTGCTGGTCGATGCGATCCGTCGCGGCGCGTCCGACATCCACT
TCGAGCCCTACGAGGACGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAAGGCGCCGGTCAAG
CTCAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGGGTCCCGCAGGACGGCCG
CATCAAGCTCAATCTGTCCAAGAACAAGCAGATCGATTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGATCG
TGTTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGTATCGACAAGCTCGGCTACGAGCCCGAGCAGCAGAAGCTGTTC
CTGGACGCGATCCACAAGCCGTACGGCATGGTCCTGGTCACTGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGCCTGCCCGGGGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
CACCCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCAGTT
CGGTGACCTTGGTCATCGCCCAGCGCCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGTCCGGTCCAATTGCCGGAGAAG
GCCTTGCTGGCCGAAGGCTTCACCGAGGCGGAGATCGCCGCCGGCGTCCAACTGTACGAAGCGGTCGGCTGCGACGAGTG
CACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGCTGCAGG
GCGGCAACGCCATGGACATCGCCAGCGCCGCGCAGAAGATCGGCGTCAAGGACCTGCGCCAGTCGGCCTTGCTCAAGGCC
CGCAACGGCATCACCAGCCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.203

98.438

0.543
  pilB Acinetobacter baumannii D1279779

54.737

98.958

0.542
  pilB Legionella pneumophila strain ERS1305867

51.142

98.785

0.505
  pilB Vibrio cholerae strain A1552

50

99.653

0.498
  pilF Neisseria gonorrhoeae MS11

49.296

98.611

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

45.789

98.958

0.453
  pilB Vibrio campbellii strain DS40M4

45.694

98.785

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

36.721

100

0.389
  pilF Thermus thermophilus HB27

40.27

90.104

0.363