Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   EP162_RS09785 Genome accession   NZ_AP018524
Coordinates   1495276..1495458 (-) Length   60 a.a.
NCBI ID   WP_013774299.1    Uniprot ID   -
Organism   Melissococcus plutonius strain DAT585     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1466375..1504638 1495276..1495458 within 0


Gene organization within MGE regions


Location: 1466375..1504638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP162_RS06310 (DAT585_1280) - 1466375..1467382 (-) 1008 WP_013774253.1 class I SAM-dependent methyltransferase -
  EP162_RS06315 (DAT585_1281) comGG 1467662..1468000 (-) 339 WP_268765908.1 competence type IV pilus minor pilin ComGG -
  EP162_RS06320 (DAT585_1282) comGF 1467997..1468437 (-) 441 WP_053078316.1 competence type IV pilus minor pilin ComGF -
  EP162_RS06325 (DAT585_1283) - 1468418..1468762 (-) 345 WP_041363232.1 hypothetical protein -
  EP162_RS06330 (DAT585_1284) comGD 1468779..1469183 (-) 405 WP_013774256.1 competence type IV pilus minor pilin ComGD -
  EP162_RS06335 (DAT585_1285) comYC 1469180..1469413 (-) 234 WP_013774257.1 competence type IV pilus major pilin ComGC Machinery gene
  EP162_RS06340 - 1469792..1470076 (-) 285 WP_041363488.1 hypothetical protein -
  EP162_RS06345 (DAT585_1287) - 1470066..1470665 (-) 600 WP_013774259.1 Panacea domain-containing protein -
  EP162_RS06350 (DAT585_1288) - 1471287..1472051 (-) 765 WP_268766437.1 peptidoglycan amidohydrolase family protein -
  EP162_RS06355 (DAT585_1289) - 1472057..1472251 (-) 195 WP_013774261.1 phage holin -
  EP162_RS06360 (DAT585_1290) - 1472253..1472495 (-) 243 WP_013774262.1 hemolysin XhlA family protein -
  EP162_RS06365 (DAT585_1291) - 1472533..1472679 (-) 147 WP_013774263.1 XkdX family protein -
  EP162_RS06370 (DAT585_1292) - 1472669..1473043 (-) 375 WP_013774264.1 hypothetical protein -
  EP162_RS06375 (DAT585_1293) - 1473063..1473524 (-) 462 WP_048590192.1 hypothetical protein -
  EP162_RS06380 (DAT585_1294) - 1473524..1474216 (-) 693 WP_013774265.1 phage baseplate upper protein -
  EP162_RS06385 (DAT585_1295) - 1474206..1474454 (-) 249 WP_013774266.1 hypothetical protein -
  EP162_RS06390 (DAT585_1296) - 1474454..1475830 (-) 1377 WP_048590191.1 phage tail protein -
  EP162_RS06395 (DAT585_1297) - 1475843..1476667 (-) 825 WP_013774268.1 phage tail family protein -
  EP162_RS06400 (DAT585_1298) - 1476667..1478523 (-) 1857 WP_158309120.1 transglycosylase SLT domain-containing protein -
  EP162_RS06405 (DAT585_1299) - 1479007..1480824 (-) 1818 WP_127468704.1 tape measure protein -
  EP162_RS06410 (DAT585_1300) - 1480945..1481562 (-) 618 Protein_1278 Gp15 family bacteriophage protein -
  EP162_RS06420 (DAT585_1302) - 1481698..1482000 (-) 303 WP_048594068.1 hypothetical protein -
  EP162_RS06430 (DAT585_1303) - 1482306..1482827 (-) 522 WP_013774273.1 phage tail tube protein -
  EP162_RS06435 (DAT585_1304) - 1482824..1483231 (-) 408 WP_048590187.1 phage tail terminator protein -
  EP162_RS06440 (DAT585_1305) - 1483231..1483584 (-) 354 WP_013774275.1 minor capsid protein -
  EP162_RS06445 (DAT585_1306) - 1483585..1483935 (-) 351 WP_013774276.1 putative minor capsid protein -
  EP162_RS09390 (DAT585_1307) - 1483932..1484135 (-) 204 WP_232038649.1 hypothetical protein -
  EP162_RS09395 (DAT585_1308) - 1484154..1484321 (-) 168 WP_013774278.1 hypothetical protein -
  EP162_RS06455 (DAT585_1309) - 1484388..1485251 (-) 864 WP_048590186.1 hypothetical protein -
  EP162_RS09710 - 1485263..1485841 (-) 579 Protein_1287 phage scaffolding protein -
  EP162_RS06470 (DAT585_1312) - 1485952..1487088 (-) 1137 Protein_1288 phage minor capsid protein -
  EP162_RS06475 (DAT585_1314) - 1487091..1488647 (-) 1557 WP_048590185.1 phage portal protein -
  EP162_RS06480 - 1488664..1489916 (-) 1253 Protein_1290 PBSX family phage terminase large subunit -
  EP162_RS06485 (DAT585_1318) - 1489982..1490491 (-) 510 WP_013774288.1 terminase small subunit -
  EP162_RS09775 - 1490727..1490813 (-) 87 WP_221293899.1 putative holin-like toxin -
  EP162_RS06490 (DAT585_1320) - 1491083..1491562 (-) 480 WP_145986062.1 hypothetical protein -
  EP162_RS06495 (DAT585_1321) - 1491936..1492355 (-) 420 WP_013774291.1 transcriptional regulator -
  EP162_RS06500 (DAT585_1322) - 1492369..1492635 (-) 267 WP_013774292.1 hypothetical protein -
  EP162_RS06505 (DAT585_1323) - 1492625..1493011 (-) 387 WP_013774293.1 hypothetical protein -
  EP162_RS06510 (DAT585_1324) - 1493012..1493212 (-) 201 WP_013774294.1 hypothetical protein -
  EP162_RS06515 (DAT585_1325) - 1493313..1494059 (-) 747 WP_013774295.1 hypothetical protein -
  EP162_RS06520 (DAT585_1326) - 1494066..1494485 (-) 420 WP_013774296.1 hypothetical protein -
  EP162_RS06525 (DAT585_1327) - 1494516..1494935 (-) 420 WP_013774297.1 RusA family crossover junction endodeoxyribonuclease -
  EP162_RS09780 (DAT585_1328) - 1494953..1495249 (-) 297 WP_013774298.1 single-stranded DNA-binding protein -
  EP162_RS09785 (DAT585_1329) ssb 1495276..1495458 (-) 183 WP_013774299.1 single-stranded DNA-binding protein Machinery gene
  EP162_RS06535 (DAT585_1330) - 1495455..1496015 (-) 561 WP_048590183.1 hypothetical protein -
  EP162_RS09790 (DAT585_1331) - 1496461..1496880 (-) 420 WP_414973931.1 phage replisome organizer N-terminal domain-containing protein -
  EP162_RS06545 (DAT585_1332) - 1496920..1497606 (-) 687 WP_013774302.1 putative HNHc nuclease -
  EP162_RS06550 (DAT585_1333) - 1497714..1498424 (-) 711 WP_231849634.1 PD-(D/E)XK nuclease-like domain-containing protein -
  EP162_RS06555 (DAT585_1334) bet 1498375..1499103 (-) 729 WP_013774304.1 phage recombination protein Bet -
  EP162_RS06560 (DAT585_1335) - 1499096..1499404 (-) 309 WP_013774305.1 hypothetical protein -
  EP162_RS09105 (DAT585_1336) - 1499459..1499599 (-) 141 WP_013774306.1 hypothetical protein -
  EP162_RS06565 (DAT585_1337) - 1499596..1499787 (-) 192 WP_013774307.1 hypothetical protein -
  EP162_RS06570 (DAT585_1338) - 1499774..1499953 (-) 180 WP_013774308.1 hypothetical protein -
  EP162_RS06575 (DAT585_1339) - 1499966..1500724 (-) 759 WP_013774309.1 phage repressor protein/antirepressor Ant -
  EP162_RS06580 (DAT585_1340) - 1500721..1500963 (-) 243 WP_013774310.1 helix-turn-helix transcriptional regulator -
  EP162_RS06585 (DAT585_1341) - 1501149..1501865 (+) 717 WP_041363490.1 XRE family transcriptional regulator -
  EP162_RS06590 (DAT585_1342) - 1501970..1502641 (+) 672 WP_053078312.1 hypothetical protein -
  EP162_RS06595 (DAT585_1343) - 1502739..1503083 (+) 345 WP_127468707.1 hypothetical protein -
  EP162_RS09795 - 1503203..1504638 (+) 1436 Protein_1317 recombinase family protein -

Sequence

Protein


Download         Length: 60 a.a.        Molecular weight: 6758.65 Da        Isoelectric Point: 9.1733

>NTDB_id=69859 EP162_RS09785 WP_013774299.1 1495276..1495458(-) (ssb) [Melissococcus plutonius strain DAT585]
MINNVVLVGRLTKDPDLRYTSGDSAVATFTLAVNRNFKNQNGDREADFVNCVIWHKPAKH

Nucleotide


Download         Length: 183 bp        

>NTDB_id=69859 EP162_RS09785 WP_013774299.1 1495276..1495458(-) (ssb) [Melissococcus plutonius strain DAT585]
ATGATAAACAATGTTGTACTAGTTGGAAGATTAACAAAGGATCCTGATTTACGCTATACATCTGGTGATTCGGCAGTAGC
AACGTTTACTTTAGCAGTCAATCGGAATTTCAAAAATCAAAACGGCGATCGAGAGGCTGATTTTGTTAATTGTGTTATTT
GGCATAAGCCAGCTAAACATTAG

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-58)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

71.667

100

0.717
  ssbA Bacillus subtilis subsp. subtilis str. 168

70

100

0.7
  ssbB Bacillus subtilis subsp. subtilis str. 168

66.667

100

0.667
  ssbB/cilA Streptococcus mitis NCTC 12261

55.932

98.333

0.55
  ssbB/cilA Streptococcus pneumoniae TIGR4

55.932

98.333

0.55
  ssbB/cilA Streptococcus mitis SK321

55.932

98.333

0.55
  ssbB/cilA Streptococcus pneumoniae Rx1

54.237

98.333

0.533
  ssbB/cilA Streptococcus pneumoniae D39

54.237

98.333

0.533
  ssbB/cilA Streptococcus pneumoniae R6

54.237

98.333

0.533
  ssbB Streptococcus sobrinus strain NIDR 6715-7

52.542

98.333

0.517
  ssbA Streptococcus mutans UA159

49.153

98.333

0.483
  ssbB Lactococcus lactis subsp. cremoris KW2

44.828

96.667

0.433
  ssb Staphylococcus aureus N315

41.379

96.667

0.4
  ssb Staphylococcus aureus MW2

39.655

96.667

0.383

Multiple sequence alignment