Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M8003_RS26770 Genome accession   NZ_CP097528
Coordinates   5816970..5817968 (-) Length   332 a.a.
NCBI ID   WP_049588310.1    Uniprot ID   -
Organism   Pseudomonas putida strain KT2440 isolate pMBEC6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5811970..5822968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8003_RS26750 (M8003_26715) argS 5813107..5814843 (+) 1737 WP_010955633.1 arginine--tRNA ligase -
  M8003_RS26755 (M8003_26720) - 5814845..5815543 (+) 699 WP_003249122.1 SPOR domain-containing protein -
  M8003_RS26760 (M8003_26725) - 5815634..5816053 (-) 420 WP_010955634.1 TM2 domain-containing protein -
  M8003_RS26765 (M8003_26730) - 5816218..5816841 (+) 624 WP_010955635.1 C40 family peptidase -
  M8003_RS26770 (M8003_26735) pilT 5816970..5817968 (-) 999 WP_049588310.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M8003_RS26775 (M8003_26740) - 5818034..5818720 (+) 687 WP_010955637.1 YggS family pyridoxal phosphate-dependent enzyme -
  M8003_RS26780 (M8003_26745) proC 5818759..5819577 (+) 819 WP_010955638.1 pyrroline-5-carboxylate reductase -
  M8003_RS26785 (M8003_26750) - 5819588..5820178 (+) 591 WP_003249110.1 YggT family protein -
  M8003_RS26790 (M8003_26755) - 5820278..5821417 (+) 1140 WP_010955639.1 homoserine O-acetyltransferase -
  M8003_RS26795 (M8003_26760) metW 5821425..5822045 (+) 621 WP_003249106.1 methionine biosynthesis protein MetW -
  M8003_RS26800 (M8003_26765) - 5822068..5822502 (+) 435 WP_010955641.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36024.71 Da        Isoelectric Point: 7.5270

>NTDB_id=689935 M8003_RS26770 WP_049588310.1 5816970..5817968(-) (pilT) [Pseudomonas putida strain KT2440 isolate pMBEC6]
MDVTDLLARAVDAGASDLHLATGQIPMLRLDGELQRMGLPPLSQGALIEGMASVLDNDQRRQWAQGDEMDLALELPALGR
FRLNLFRQLNGPAVTFRLIPGRIATVSELDLADVFQAVAHCRDGLILVGGPTGSGKSSTLATLLDQLNRERALHIITLED
PVEIIHDSQRSLVNQREMGRHSRGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHARSATSSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRVGGGRVAAREVLVATPAVRNLVREGRLAQLSSVMQGGAAEGMLTMEGAL
RRLRERGLIKGL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=689935 M8003_RS26770 WP_049588310.1 5816970..5817968(-) (pilT) [Pseudomonas putida strain KT2440 isolate pMBEC6]
ATGGATGTGACCGACCTGTTGGCCCGAGCTGTGGATGCGGGGGCTTCAGACCTGCACCTGGCGACGGGCCAGATTCCGAT
GCTGCGCCTGGATGGCGAGTTGCAGCGTATGGGCTTGCCGCCGTTGAGCCAGGGTGCCTTGATCGAGGGCATGGCCAGCG
TGCTGGATAACGATCAGCGCCGGCAGTGGGCCCAAGGCGATGAAATGGACCTTGCGCTGGAGTTGCCGGCGCTTGGGCGC
TTTCGGCTGAACCTGTTTCGCCAGTTGAATGGCCCGGCGGTTACCTTTCGGCTGATCCCGGGGCGCATTGCGACGGTGAG
TGAGCTGGACCTTGCGGATGTGTTTCAAGCTGTTGCGCATTGCCGGGATGGCTTGATCCTCGTGGGCGGGCCGACCGGCA
GTGGCAAGTCCAGCACCCTGGCAACGCTGCTCGACCAGCTGAACCGCGAGCGGGCGCTGCACATCATTACCCTGGAAGAC
CCTGTCGAGATCATCCATGACAGCCAGCGCAGCCTGGTCAACCAGCGTGAGATGGGGCGCCATAGTCGCGGCTTTGCTCA
GGGGCTGCGCAGTGCGCTGCGCCAGGACCCGGACGTGATCATGATCGGCGAGTTGCGCGACCTGGAAACCATTCGCCTGG
CCCTGCGCGCGGCCGAAACCGGGCACCTGGTGCTGGCAACCGTGCATGCGCGTTCGGCGACCAGCAGCATCGACAGGTTG
GTGGAGGTGTTTGCTGCCGAGGAAAAGCCGTTGGTGCGGGCGATGCTGGCCGAGTCGCTGCGCCTGGTGGTCGCGCAGGT
GCTGGTCAGGCGCGTAGGGGGTGGGCGGGTGGCGGCGCGGGAAGTGCTGGTGGCGACGCCGGCGGTGCGTAACCTGGTTC
GGGAGGGGCGGTTGGCGCAGTTGTCTTCAGTGATGCAGGGCGGGGCGGCGGAGGGGATGCTGACGATGGAGGGGGCGTTG
CGGAGGCTGAGGGAGCGAGGGTTGATCAAAGGCTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.76

99.096

0.503
  pilT Acinetobacter nosocomialis M2

50.152

99.096

0.497
  pilT Acinetobacter baumannii D1279779

50.152

99.096

0.497
  pilT Acinetobacter baumannii strain A118

50.152

99.096

0.497
  pilT Acinetobacter baylyi ADP1

49.697

99.398

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.848

99.096

0.494
  pilT Neisseria meningitidis 8013

49.091

99.398

0.488
  pilT Neisseria gonorrhoeae MS11

48.788

99.398

0.485
  pilT Vibrio cholerae strain A1552

48.936

99.096

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.936

99.096

0.485
  pilT Legionella pneumophila strain ERS1305867

48.632

99.096

0.482
  pilT Legionella pneumophila strain Lp02

48.632

99.096

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.429

100

0.47
  pilU Pseudomonas stutzeri DSM 10701

37.879

99.398

0.377