Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M5524_RS06310 Genome accession   NZ_CP097466
Coordinates   1466823..1468004 (+) Length   393 a.a.
NCBI ID   WP_373990597.1    Uniprot ID   -
Organism   Duganella sp. BuS-21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1461823..1473004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5524_RS06285 (M5524_06260) - 1462483..1463745 (-) 1263 WP_373990592.1 parallel beta-helix domain-containing protein -
  M5524_RS06290 (M5524_06265) - 1463837..1464340 (-) 504 WP_373990593.1 hypothetical protein -
  M5524_RS06295 (M5524_06270) - 1464411..1464764 (-) 354 WP_373990594.1 YqjD family protein -
  M5524_RS06300 (M5524_06275) ubiA 1464855..1465712 (-) 858 WP_373990595.1 4-hydroxybenzoate octaprenyltransferase -
  M5524_RS06305 (M5524_06280) - 1465779..1466714 (-) 936 WP_373990596.1 hydrogen peroxide-inducible genes activator -
  M5524_RS06310 (M5524_06285) pilU 1466823..1468004 (+) 1182 WP_373990597.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5524_RS06315 (M5524_06290) - 1468026..1468505 (+) 480 WP_373990598.1 adenylyltransferase/cytidyltransferase family protein -
  M5524_RS06320 (M5524_06295) - 1468502..1468789 (+) 288 WP_373990599.1 hypothetical protein -
  M5524_RS06325 (M5524_06300) trmL 1469047..1469517 (-) 471 WP_373990600.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  M5524_RS06330 (M5524_06305) - 1469538..1470272 (-) 735 WP_373990601.1 phosphoribosyltransferase family protein -
  M5524_RS06335 (M5524_06310) - 1470369..1471292 (+) 924 WP_373990602.1 methyltransferase domain-containing protein -
  M5524_RS06340 (M5524_06315) coxB 1471635..1472876 (+) 1242 WP_373990603.1 cytochrome c oxidase subunit II -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 43462.74 Da        Isoelectric Point: 6.0967

>NTDB_id=689578 M5524_RS06310 WP_373990597.1 1466823..1468004(+) (pilU) [Duganella sp. BuS-21]
MSSTFGPEEAQAYLHKLLKVMHHTGGSDLFISADFPPSMKSQGAMKPLSQQKLTGGVTRALALSIMNEKQQAEFEAELEC
NFAISLPEVCRFRVNVFVQQQNVAMVIRTIASEIPTLEKLELPEVLKDIIMTKRGLVLVVGGTGSGKSTTLAAMIDYRNA
NTAGHIITVEDPVEYVHKNKGCLVTHREVGVDTRSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLIRTEDGKGRKAAIEILLNTPTIAEMIFKGSFQAIKEIMHKS
RELGMCTFDQALYELYNKGYIAYEEALRNADSANGLRLQIKLHGGRPEPGEGGGAFGALSIEEETAEDDEPKT

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=689578 M5524_RS06310 WP_373990597.1 1466823..1468004(+) (pilU) [Duganella sp. BuS-21]
ATGTCCTCCACATTTGGCCCGGAAGAAGCGCAGGCATATCTGCACAAGCTGCTCAAGGTGATGCATCACACCGGCGGCTC
GGACTTGTTCATCTCGGCCGATTTCCCGCCAAGCATGAAGTCGCAGGGCGCGATGAAGCCGCTGAGCCAGCAGAAGCTGA
CCGGCGGCGTGACGCGCGCGTTGGCGCTGTCAATCATGAACGAGAAGCAGCAGGCCGAATTCGAGGCCGAGCTGGAATGC
AACTTTGCCATTTCGCTACCGGAGGTGTGCCGCTTCCGCGTCAACGTCTTCGTGCAGCAGCAGAACGTGGCGATGGTGAT
CCGCACCATCGCCTCCGAAATTCCGACGCTGGAAAAGCTGGAACTGCCGGAGGTGCTCAAGGACATCATCATGACCAAGC
GTGGGTTGGTGTTGGTGGTGGGCGGCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACCGCAACGCC
AACACGGCCGGCCACATCATCACGGTGGAAGACCCGGTCGAGTATGTGCACAAGAACAAGGGCTGCCTGGTCACGCACCG
CGAAGTGGGCGTCGACACACGCTCCTGGCACAACGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATTTTGATCG
GCGAGATCCGCGACACCGAAACCATGGAGCACGCGATCGCGTTTGCCGAAACCGGTCACCTGTGCCTGGGTACGCTGCAC
GCCAACAACGCCAACCAGACCATGGACCGCATCATCAACTTCTTCCCGGAGGAGCGCCGCAACCAACTGCTGATGGACCT
GTCGTCGAATCTGCGCGCCATCGTCTCGCAGCGGCTGATCCGCACCGAGGACGGCAAGGGGCGCAAGGCGGCCATTGAAA
TCCTGCTCAACACGCCGACCATCGCCGAGATGATCTTCAAAGGGAGCTTCCAGGCGATCAAGGAAATCATGCACAAGTCG
CGCGAGCTGGGCATGTGCACCTTCGACCAGGCGCTGTACGAACTTTATAATAAGGGCTACATCGCCTACGAAGAGGCGCT
GCGCAATGCCGACTCGGCCAACGGCCTGCGCCTGCAGATCAAGCTGCACGGCGGGCGGCCCGAGCCGGGCGAGGGCGGCG
GCGCGTTCGGCGCGCTGAGCATCGAAGAAGAAACAGCCGAAGACGACGAACCGAAAACCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.885

88.804

0.532
  pilU Acinetobacter baylyi ADP1

54.286

89.059

0.483
  pilU Vibrio cholerae strain A1552

49.865

94.402

0.471
  pilT Pseudomonas aeruginosa PAK

43.881

85.242

0.374
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

85.751

0.374
  pilT Legionella pneumophila strain ERS1305867

44.377

83.715

0.372
  pilT Legionella pneumophila strain Lp02

44.377

83.715

0.372
  pilT Pseudomonas stutzeri DSM 10701

42.985

85.242

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.688

81.425

0.364
  pilT Vibrio cholerae strain A1552

44.688

81.425

0.364
  pilT Acinetobacter baumannii D1279779

43.202

84.224

0.364
  pilT Acinetobacter baumannii strain A118

43.202

84.224

0.364
  pilT Acinetobacter nosocomialis M2

43.202

84.224

0.364
  pilT Acinetobacter baylyi ADP1

44.937

80.407

0.361