Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   M4X66_RS00915 Genome accession   NZ_CP097286
Coordinates   203888..204325 (+) Length   145 a.a.
NCBI ID   WP_249583479.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain JACO-C-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 205071..205637 203888..204325 flank 746


Gene organization within MGE regions


Location: 203888..205637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4X66_RS00915 pilA2 203888..204325 (+) 438 WP_249583479.1 pilin Machinery gene
  M4X66_RS00920 - 205071..205637 (-) 567 WP_249583480.1 recombinase family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14931.09 Da        Isoelectric Point: 9.0030

>NTDB_id=688256 M4X66_RS00915 WP_249583479.1 203888..204325(+) (pilA2) [Pseudomonas viridiflava strain JACO-C-5]
MKVQKTLQKGFTLIELMIVVAIVGILAAVAIPAYQDYTIRAQVAELATLADGAKVAVSETYQTTGAFPTSNATANYGGAS
GTYTTSVTIGAGGVITATTINGAPVNNAIRNQTFVLTPVLGTNNSLGWTCTAGTIVAQYRPANCR

Nucleotide


Download         Length: 438 bp        

>NTDB_id=688256 M4X66_RS00915 WP_249583479.1 203888..204325(+) (pilA2) [Pseudomonas viridiflava strain JACO-C-5]
ATGAAAGTACAAAAAACGCTACAAAAAGGTTTTACGCTGATCGAACTGATGATCGTCGTAGCGATCGTGGGCATTCTCGC
TGCGGTGGCCATTCCTGCATATCAGGACTACACAATCCGCGCTCAGGTAGCAGAGCTGGCGACGCTTGCGGATGGTGCCA
AGGTCGCGGTTAGCGAGACTTACCAGACTACTGGCGCTTTTCCTACAAGCAACGCTACTGCAAACTACGGTGGCGCGTCT
GGTACTTACACTACCTCCGTTACAATTGGAGCTGGCGGAGTTATCACCGCAACTACAATTAACGGAGCACCCGTGAATAA
TGCGATTCGTAACCAGACTTTTGTACTGACTCCGGTTCTAGGAACAAACAACTCTCTTGGCTGGACATGTACTGCAGGTA
CAATCGTCGCTCAATATCGTCCAGCCAACTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-30)

Pilin (bacterial filament) (Pilin)

(40-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

57.554

95.862

0.552
  pilA2 Legionella pneumophila strain ERS1305867

56.835

95.862

0.545
  pilA Ralstonia pseudosolanacearum GMI1000

39.773

100

0.483
  pilE Neisseria gonorrhoeae MS11

50.388

88.966

0.448
  pilE Neisseria gonorrhoeae strain FA1090

50.781

88.276

0.448
  pilA/pilA1 Eikenella corrodens VA1

39.241

100

0.428
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.155

100

0.428
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.789

100

0.428
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.541

100

0.414
  pilA Vibrio campbellii strain DS40M4

44.361

91.724

0.407
  comP Acinetobacter baylyi ADP1

40

100

0.4
  pilA Acinetobacter baumannii strain A118

36.735

100

0.372
  pilA Haemophilus influenzae 86-028NP

36.986

100

0.372
  pilA Haemophilus influenzae Rd KW20

37.063

98.621

0.366