Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M5C98_RS21100 Genome accession   NZ_CP097267
Coordinates   4639491..4640627 (+) Length   378 a.a.
NCBI ID   WP_272549389.1    Uniprot ID   -
Organism   Acidovorax sp. NCPPB 3576     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4634491..4645627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5C98_RS21080 (M5C98_21080) - 4635292..4636911 (+) 1620 WP_272549384.1 methyl-accepting chemotaxis protein -
  M5C98_RS21085 (M5C98_21085) - 4636930..4637652 (-) 723 WP_272549385.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5C98_RS21090 (M5C98_21090) pilT 4637692..4638735 (+) 1044 WP_092744851.1 type IV pilus twitching motility protein PilT Machinery gene
  M5C98_RS21095 (M5C98_21095) - 4638815..4639453 (+) 639 WP_272549388.1 cyclic nucleotide-binding domain-containing protein -
  M5C98_RS21100 (M5C98_21100) pilU 4639491..4640627 (+) 1137 WP_272549389.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5C98_RS21105 (M5C98_21105) - 4640695..4641627 (+) 933 WP_272549390.1 NAD(P)-dependent oxidoreductase -
  M5C98_RS21110 (M5C98_21110) - 4641723..4642391 (-) 669 WP_272549391.1 BON domain-containing protein -
  M5C98_RS21115 (M5C98_21115) - 4642400..4642996 (-) 597 WP_272549392.1 SIS domain-containing protein -
  M5C98_RS21120 (M5C98_21120) - 4643099..4643482 (-) 384 WP_272549393.1 YraN family protein -
  M5C98_RS21125 (M5C98_21125) rsmI 4643482..4644402 (+) 921 WP_272549395.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41790.28 Da        Isoelectric Point: 6.9079

>NTDB_id=688225 M5C98_RS21100 WP_272549389.1 4639491..4640627(+) (pilU) [Acidovorax sp. NCPPB 3576]
MERDQASKFINDLLKLMVSRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQSRVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRGMISQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGDVVEIKEIMKKSRNLG
MQTFDQSLFDLFEANVITYEDALRNADSLNDLRLQIKLGSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=688225 M5C98_RS21100 WP_272549389.1 4639491..4640627(+) (pilU) [Acidovorax sp. NCPPB 3576]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCGGCGGCAGCGACCTGTTCAT
CACCGCCGAGTTTCCGCCGGCGATCAAGGTGGACGGCAAAGTCACCAAGGTGTCGCCTCAGCCGCTCACGCCCGCCCACA
CGCTGACGCTGGCGCGCGCCATCATGAGCGACAAGCAGGTGGCGGACTTCGAGCGCACGAAGGAGTGCAACTTCGCCATC
TCGCCCGCAGGCATCGGGCGCTTTCGCGTGAACGCGTTCATCCAGCAAAGCCGGGTCGGCATGGTGCTGCGGACCATTCC
GCTCACGCTGCCCACCATTGACGGGCTGGGCGTGCCGCAGGTGCTCAAGGAAGTCACGATGACCAAGCGGGGCCTGTGCA
TCCTGGTGGGTGCCACGGGCTCGGGCAAATCGACCACGCTGGCGGCGATGGTGGACTGGCGCAACGAAAATTCCTTCGGC
CACATCATCACGGTGGAAGACCCGATCGAGTTCGTGCACCCGCACAAGAACTGCGTCGTCACCCAGCGCGAGGTGGGACT
CGATACCGACAGCTGGGAAGCCGCCCTCAAGAACACGCTACGGCAGGCGCCGGACGTTATCCTGATGGGCGAGATCCGCG
ACCGCGAAACCATGGAGCACGCGGTCGCTTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTTCCGGAAGAACGCCGCGCGCAGCTCCTCATGGACCTGTCGCTCAACCT
GCGCGGCATGATCTCCCAGCGCCTCATCCCCAAGCAGGATGGCAAGGGGCGGGCCGCCGCAGTGGAGGTGATGCTGAACA
CGCCGTTGATCGCCGACCTGATCTTCAAGGGCGACGTCGTCGAGATCAAAGAGATCATGAAGAAGAGCCGCAACCTTGGC
ATGCAGACGTTCGATCAATCGCTGTTCGACCTGTTCGAGGCCAACGTCATCACCTACGAAGACGCGCTGCGCAATGCCGA
CTCGCTGAACGACCTGCGGCTGCAGATCAAGCTGGGCAGCCAGCGCGCGAAGACCACTGATTTGGCCTCGGGCACCGAGC
ACTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

57.263

94.709

0.542
  pilU Vibrio cholerae strain A1552

54.598

92.063

0.503
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

89.153

0.394
  pilT Acinetobacter baumannii strain A118

44.745

88.095

0.394
  pilT Acinetobacter nosocomialis M2

44.745

88.095

0.394
  pilT Acinetobacter baumannii D1279779

44.745

88.095

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Legionella pneumophila strain Lp02

43.544

88.095

0.384
  pilT Legionella pneumophila strain ERS1305867

43.544

88.095

0.384
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

87.831

0.368
  pilT Vibrio cholerae strain A1552

41.867

87.831

0.368