Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M0D43_RS14860 Genome accession   NZ_CP096142
Coordinates   3462825..3463958 (-) Length   377 a.a.
NCBI ID   WP_252162014.1    Uniprot ID   A0A9Q9J0C1
Organism   Xanthomonas prunicola strain CIX249     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3457825..3468958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0D43_RS14835 (M0D43_14810) - 3457923..3458258 (-) 336 WP_252162012.1 phasin family protein -
  M0D43_RS14840 (M0D43_14815) queD 3458451..3458804 (-) 354 WP_101362487.1 6-carboxytetrahydropterin synthase QueD -
  M0D43_RS14845 (M0D43_14820) - 3458922..3460307 (-) 1386 WP_101362151.1 DEAD/DEAH box helicase -
  M0D43_RS14850 (M0D43_14825) - 3460618..3461676 (+) 1059 WP_260808277.1 fumarylacetoacetate hydrolase family protein -
  M0D43_RS14855 (M0D43_14830) maiA 3461720..3462382 (+) 663 WP_260807319.1 maleylacetoacetate isomerase -
  M0D43_RS14860 (M0D43_14835) pilU 3462825..3463958 (-) 1134 WP_252162014.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M0D43_RS14865 (M0D43_14840) - 3464057..3464440 (+) 384 WP_010371890.1 DUF4398 domain-containing protein -
  M0D43_RS14870 (M0D43_14845) - 3464437..3465327 (+) 891 WP_252162015.1 hypothetical protein -
  M0D43_RS14875 (M0D43_14850) - 3465679..3465897 (+) 219 WP_003484103.1 YdcH family protein -
  M0D43_RS14880 (M0D43_14855) - 3466137..3467507 (+) 1371 WP_260807321.1 pyridoxal-phosphate dependent enzyme -
  M0D43_RS14885 (M0D43_14860) - 3467596..3468792 (+) 1197 WP_260807322.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41721.12 Da        Isoelectric Point: 6.1401

>NTDB_id=679669 M0D43_RS14860 WP_252162014.1 3462825..3463958(-) (pilU) [Xanthomonas prunicola strain CIX249]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYEVASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=679669 M0D43_RS14860 WP_252162014.1 3462825..3463958(-) (pilU) [Xanthomonas prunicola strain CIX249]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTATCCGCTCGGCAATACGGGGCTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCCGACGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCATGCCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCAGAGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGTCACCTGTGCCTGGCCACGTTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTGTCGCTGCG
CCTGGTCAAGGGTGTGGACGGACGCCGCCGGCCGGCGACCGAAGTGCTGCTCAACACGCCAATGATCCGCGACCTGCTGC
GGCGCGGCCAGGTGCACGAGGTCAAGGCGGCGATGGAAGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGTCTG
TTCCGGATGGTCAAATCAGGGGAAATCGAGCAGGAAGAAGCGCTGCGCGCTGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTCTCCGAAGGCGGCTCCGGCGAGCACGACCCGTATGCGGATTACGAAGTGGCCTCGGCGCCGAAGATCAGTC
ACGGGTTCATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.153

93.899

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.448
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Vibrio cholerae strain A1552

40.841

88.329

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.329

0.361