Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OG285_RS34535 Genome accession   NZ_CP109450
Coordinates   7634199..7635311 (-) Length   370 a.a.
NCBI ID   WP_371793291.1    Uniprot ID   -
Organism   Streptomyces sp. NBC_01471     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7629199..7640311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OG285_RS34515 (OG285_34490) - 7629983..7630603 (-) 621 WP_371793288.1 TetR/AcrR family transcriptional regulator -
  OG285_RS34520 (OG285_34495) - 7630708..7631769 (+) 1062 WP_371793678.1 medium chain dehydrogenase/reductase family protein -
  OG285_RS34525 (OG285_34500) - 7631873..7632706 (-) 834 WP_371793289.1 aldo/keto reductase -
  OG285_RS34530 (OG285_34505) - 7632928..7634118 (+) 1191 WP_371793290.1 acyl-CoA dehydrogenase family protein -
  OG285_RS34535 (OG285_34510) pilT 7634199..7635311 (-) 1113 WP_371793291.1 type IV pilus twitching motility protein PilT Machinery gene
  OG285_RS34540 (OG285_34515) - 7635344..7636063 (+) 720 WP_371793292.1 hypothetical protein -
  OG285_RS34545 (OG285_34520) - 7636068..7636454 (+) 387 WP_371793293.1 excalibur calcium-binding domain-containing protein -
  OG285_RS34550 (OG285_34525) - 7636835..7637782 (+) 948 WP_371793294.1 oxygenase MpaB family protein -
  OG285_RS34555 (OG285_34530) - 7637764..7640001 (+) 2238 WP_356832010.1 serine/threonine-protein kinase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 39966.51 Da        Isoelectric Point: 6.5807

>NTDB_id=676708 OG285_RS34535 WP_371793291.1 7634199..7635311(-) (pilT) [Streptomyces sp. NBC_01471]
MTKHATLEATMPGPSSAIIDHLLGELWERGGSDLLLTAGSAPFLRVDGALRPVEGAALAEPEVDRLVTGVLGRELTERFR
REKQVDFAFSWGEKARLRGNAFVQRGASALALRIIPFDVPSSGQLGLPPAVIGWAGLPRGFVLVTGPTGSGKSTSLAALL
DYVNTHRSVHILTIEDPIEYLHRHKRSAVNQREVGTDTDSFPAALRSALREDPDVLLLGEMRDPESISAALTIAETGHLV
FATLHTNDTSQTLDRIVDVFPAEQQPQIRLQLAHTLVGILNQTLIPRIGGGRVAAFEVLAGTSAIRNLIREGKTRQIRNM
VSTGHREGMQTLETSINALVAAGTIAYEEAVAYTAYPEDIQRPHVPAGRT

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=676708 OG285_RS34535 WP_371793291.1 7634199..7635311(-) (pilT) [Streptomyces sp. NBC_01471]
ATGACCAAACACGCCACCTTGGAGGCCACCATGCCCGGGCCCAGTTCCGCGATCATCGACCACCTGCTCGGTGAGCTGTG
GGAGCGGGGCGGCAGCGACCTGCTGCTCACCGCCGGATCGGCGCCGTTCCTCCGCGTCGACGGCGCCCTGCGACCGGTCG
AGGGGGCCGCGCTGGCCGAGCCGGAGGTCGATCGCCTGGTGACCGGGGTGCTGGGCAGGGAACTCACGGAACGGTTCCGA
CGGGAGAAGCAGGTCGATTTCGCCTTCAGCTGGGGGGAGAAGGCAAGGTTGCGCGGGAACGCATTCGTGCAGCGCGGCGC
GTCGGCGCTGGCGCTGCGGATCATCCCGTTCGACGTGCCGTCGTCCGGGCAACTCGGGCTCCCCCCGGCCGTCATCGGAT
GGGCGGGGCTGCCGCGGGGGTTCGTGCTGGTCACCGGGCCGACCGGGTCGGGGAAGTCGACAAGCCTGGCGGCGCTCCTC
GACTACGTCAACACGCACCGGTCGGTGCACATCCTCACCATCGAGGACCCGATCGAGTACCTGCACCGGCACAAGCGCTC
GGCGGTCAACCAGCGCGAGGTCGGCACCGACACCGACTCGTTCCCCGCCGCGCTGCGCTCGGCCCTGCGCGAGGATCCCG
ATGTCCTGCTCCTCGGCGAGATGCGCGACCCGGAGAGCATCTCCGCGGCCCTGACGATCGCCGAGACCGGACACCTGGTG
TTCGCCACCCTGCACACCAATGACACGTCGCAGACACTGGACCGCATCGTCGACGTGTTCCCGGCCGAGCAACAGCCGCA
GATCCGGCTGCAATTGGCGCACACCCTGGTCGGCATCCTCAACCAGACGCTCATCCCCCGGATCGGCGGGGGCAGGGTGG
CCGCCTTCGAGGTACTGGCAGGCACGTCGGCGATCCGCAACCTGATCCGCGAGGGCAAGACCCGCCAGATCCGCAACATG
GTCTCGACCGGCCACCGTGAAGGCATGCAGACCCTTGAGACCAGCATCAACGCCCTCGTCGCGGCAGGCACCATCGCCTA
CGAGGAGGCCGTGGCGTACACGGCCTACCCGGAGGACATCCAGCGGCCCCACGTCCCGGCCGGCCGCACCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(138-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

49.123

92.432

0.454
  pilT Pseudomonas stutzeri DSM 10701

48.688

92.703

0.451
  pilT Acinetobacter baumannii D1279779

48.529

91.892

0.446
  pilT Acinetobacter baumannii strain A118

48.529

91.892

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.81

90.811

0.443
  pilT Acinetobacter nosocomialis M2

48.235

91.892

0.443
  pilT Acinetobacter baylyi ADP1

48.235

91.892

0.443
  pilT Neisseria meningitidis 8013

48.503

90.27

0.438
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.892

89.73

0.43
  pilT Vibrio cholerae strain A1552

47.892

89.73

0.43
  pilT Neisseria gonorrhoeae MS11

47.748

90

0.43
  pilT Legionella pneumophila strain Lp02

47.59

89.73

0.427
  pilT Legionella pneumophila strain ERS1305867

47.59

89.73

0.427
  pilU Pseudomonas stutzeri DSM 10701

41.159

93.243

0.384