Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L9B60_RS22865 Genome accession   NZ_CP094975
Coordinates   5012879..5014036 (+) Length   385 a.a.
NCBI ID   WP_249673250.1    Uniprot ID   -
Organism   Pseudomonas abieticivorans strain PIA16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5007879..5019036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L9B60_RS22845 - 5008119..5009333 (+) 1215 WP_249673248.1 amino acid deaminase -
  L9B60_RS22850 - 5009347..5010300 (+) 954 WP_283780526.1 sugar kinase -
  L9B60_RS22855 - 5010288..5010563 (-) 276 WP_007932676.1 peptidylprolyl isomerase -
  L9B60_RS22860 - 5010604..5012790 (-) 2187 WP_249673249.1 ATP-binding protein -
  L9B60_RS22865 pilU 5012879..5014036 (+) 1158 WP_249673250.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L9B60_RS22870 - 5014111..5014881 (-) 771 WP_249673251.1 3-hydroxybutyrate dehydrogenase -
  L9B60_RS22875 - 5014955..5016529 (-) 1575 WP_249679952.1 ABC transporter ATP-binding protein -
  L9B60_RS22880 - 5016540..5017562 (-) 1023 WP_249673252.1 ABC transporter permease -
  L9B60_RS22885 - 5017562..5018623 (-) 1062 WP_249673253.1 microcin C ABC transporter permease YejB -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 42418.34 Da        Isoelectric Point: 5.9072

>NTDB_id=674668 L9B60_RS22865 WP_249673250.1 5012879..5014036(+) (pilU) [Pseudomonas abieticivorans strain PIA16]
MDIQALLKTLASQDGSDLYLSTGAPPCAKFNGVLKPLGAEALKPGEVALIAANIMDAEQRADFERDLEMNLAMSLPGVGR
FRVNIFKQRNEVSIVARNIKLDIPRFEDLKLPPVLLDVVMQKRGLVLFVGATGSGKSTSLAALIDYRNRSSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFQAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLQAFVSQRLVKTVDGKRRAAVEVMLGTPAIRDFIHRNEFAELKGIMEKSGTLGMQTFDQAL
YDLAVEGAISEDEALKNADSQNNVRLRLKLHREGGFEQTTAPAPPTPAADVDSADWGLVEDDRPT

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=674668 L9B60_RS22865 WP_249673250.1 5012879..5014036(+) (pilU) [Pseudomonas abieticivorans strain PIA16]
ATGGACATCCAAGCCCTGCTCAAAACCCTGGCCAGCCAGGACGGTTCCGACCTGTACCTGTCGACCGGGGCGCCGCCGTG
CGCCAAGTTCAATGGCGTGCTCAAGCCGCTGGGGGCCGAGGCGCTGAAGCCTGGCGAAGTCGCGCTGATCGCCGCCAACA
TCATGGACGCCGAACAGCGTGCCGACTTCGAGCGCGACCTGGAGATGAACCTGGCCATGTCCTTGCCGGGGGTCGGGCGC
TTTCGGGTCAATATCTTCAAGCAGCGCAACGAAGTGTCGATCGTGGCGCGCAACATCAAGTTGGACATCCCGCGTTTCGA
AGACCTCAAGCTGCCGCCGGTGTTGCTCGACGTGGTGATGCAAAAGCGCGGGCTGGTGCTGTTCGTCGGCGCCACCGGCT
CGGGCAAGTCCACGTCGCTTGCCGCCTTGATCGATTACCGTAACCGTAGCAGTAGCGGCCACATCATTACCATCGAAGAT
CCGGTGGAGTATATCCACCGCCACAAAAAATCGATCATCAACCAGCGCGAAGTGGGCGTGGACACCCGCAGTTTCCAGGC
AGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATATTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCACG
CCCTGGCCTTTGCCGACACCGGCCACCTGGCGATTTCCACCTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATT
ATCAACTTTTTCCCCGAAGACCGCCGCCCGCAATTGCTCAACGACCTGGGCAACAACCTGCAGGCGTTCGTGTCCCAGCG
CCTGGTCAAAACCGTGGACGGCAAGCGACGGGCGGCGGTGGAAGTGATGCTCGGCACCCCGGCGATCCGCGACTTCATCC
ACCGCAATGAATTCGCCGAGCTCAAGGGCATCATGGAAAAGTCCGGCACCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TATGACCTGGCCGTGGAAGGGGCGATCAGTGAGGATGAGGCGCTGAAAAATGCCGATTCGCAGAACAACGTGCGGCTGAG
GCTGAAGTTGCACCGCGAAGGTGGGTTCGAGCAGACAACGGCGCCGGCGCCGCCCACGCCTGCGGCGGATGTGGACAGCG
CCGATTGGGGGTTGGTGGAGGATGATCGGCCGACGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.354

94.026

0.53
  pilU Acinetobacter baylyi ADP1

52.661

92.727

0.488
  pilU Vibrio cholerae strain A1552

51.275

91.688

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.16

97.662

0.392
  pilT Pseudomonas stutzeri DSM 10701

43.732

89.091

0.39
  pilT Pseudomonas aeruginosa PAK

42.857

89.091

0.382
  pilT Vibrio cholerae strain A1552

42.12

90.649

0.382
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.12

90.649

0.382
  pilT Legionella pneumophila strain ERS1305867

42.515

86.753

0.369
  pilT Acinetobacter baumannii D1279779

42.515

86.753

0.369
  pilT Acinetobacter baumannii strain A118

42.515

86.753

0.369
  pilT Acinetobacter nosocomialis M2

42.515

86.753

0.369
  pilT Legionella pneumophila strain Lp02

42.515

86.753

0.369
  pilT Acinetobacter baylyi ADP1

42.424

85.714

0.364