Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LEN_RS25835 Genome accession   NZ_AP014940
Coordinates   1239249..1239674 (-) Length   141 a.a.
NCBI ID   WP_096376952.1    Uniprot ID   A0AAU9AD11
Organism   Lysobacter enzymogenes strain M497-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1234249..1244674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEN_RS05280 (LEN_1086) - 1235428..1237329 (-) 1902 WP_096376949.1 hypothetical protein -
  LEN_RS05285 (LEN_1087) - 1237361..1237774 (-) 414 WP_096376950.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LEN_RS25830 (LEN_1088) - 1237919..1238344 (-) 426 WP_096376951.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LEN_RS25835 (LEN_1089) pilA 1239249..1239674 (-) 426 WP_096376952.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LEN_RS05300 (LEN_1090) pilR 1240339..1241748 (-) 1410 WP_074862710.1 sigma-54 dependent transcriptional regulator Regulator
  LEN_RS05305 (LEN_1091) - 1241846..1243471 (-) 1626 WP_096376953.1 ATP-binding protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14946.13 Da        Isoelectric Point: 7.5863

>NTDB_id=67464 LEN_RS25835 WP_096376952.1 1239249..1239674(-) (pilA) [Lysobacter enzymogenes strain M497-1]
MNKQQGFTLIELMIVIAILGILIAIALPAYQDYTVRTKNAECLNASAAAKLAVTETAQDRGGLDKVTDTNTGYAFTASKY
CASIKVETKDGIIVTEGTADTGGNPIAKFKLTPTAADGRIDWACTETNGAKKSQLPAECRK

Nucleotide


Download         Length: 426 bp        

>NTDB_id=67464 LEN_RS25835 WP_096376952.1 1239249..1239674(-) (pilA) [Lysobacter enzymogenes strain M497-1]
ATGAACAAGCAGCAAGGCTTCACCCTGATCGAACTGATGATCGTCATCGCGATCCTCGGCATCCTGATCGCCATCGCTCT
GCCGGCCTACCAGGACTACACCGTCCGCACCAAGAACGCCGAGTGCCTGAATGCCTCCGCCGCCGCCAAGCTGGCCGTGA
CGGAAACCGCGCAGGACCGCGGCGGGCTGGACAAGGTCACCGACACGAACACCGGGTATGCATTCACCGCCAGCAAGTAC
TGCGCGAGCATCAAGGTCGAGACCAAGGACGGCATCATCGTCACCGAGGGCACTGCCGATACCGGCGGTAACCCCATCGC
AAAGTTCAAGCTGACCCCGACCGCCGCCGACGGCCGCATCGATTGGGCTTGCACCGAGACCAACGGCGCCAAGAAGTCGC
AGTTGCCGGCCGAATGTAGGAAGTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

40.351

100

0.489
  pilA2 Legionella pneumophila str. Paris

47.143

99.291

0.468
  comP Acinetobacter baylyi ADP1

43.046

100

0.461
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.105

100

0.454
  pilA2 Legionella pneumophila strain ERS1305867

45.324

98.582

0.447
  pilE Neisseria gonorrhoeae strain FA1090

39.241

100

0.44
  pilA Pseudomonas aeruginosa PAK

39.355

100

0.433
  pilA/pilA1 Eikenella corrodens VA1

39.865

100

0.418
  pilA Vibrio campbellii strain DS40M4

40.136

100

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.304

97.872

0.404
  pilA Haemophilus influenzae 86-028NP

38.028

100

0.383
  pilA Glaesserella parasuis strain SC1401

36.62

100

0.369
  pilE Neisseria gonorrhoeae MS11

40.157

90.071

0.362

Multiple sequence alignment