Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LEN_RS04610 Genome accession   NZ_AP014940
Coordinates   1083734..1084849 (-) Length   371 a.a.
NCBI ID   WP_096376847.1    Uniprot ID   A0AAU9AC17
Organism   Lysobacter enzymogenes strain M497-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1078734..1089849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEN_RS04580 (LEN_0942) rplM 1079673..1080101 (+) 429 WP_074862335.1 50S ribosomal protein L13 -
  LEN_RS04585 (LEN_0943) rpsI 1080117..1080509 (+) 393 WP_074862338.1 30S ribosomal protein S9 -
  LEN_RS04595 (LEN_0944) - 1080920..1081423 (-) 504 WP_096376845.1 GNAT family N-acetyltransferase -
  LEN_RS04600 (LEN_0946) - 1081665..1082645 (+) 981 WP_096376846.1 fumarylacetoacetate hydrolase family protein -
  LEN_RS04605 (LEN_0947) maiA 1082885..1083547 (+) 663 WP_074862347.1 maleylacetoacetate isomerase -
  LEN_RS04610 (LEN_0948) pilU 1083734..1084849 (-) 1116 WP_096376847.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LEN_RS04615 (LEN_0949) - 1084842..1085330 (+) 489 WP_232518415.1 DUF4398 domain-containing protein -
  LEN_RS04620 (LEN_0950) - 1085327..1085956 (+) 630 WP_096376849.1 hypothetical protein -
  LEN_RS04625 (LEN_0951) - 1086640..1086855 (+) 216 WP_074862358.1 YdcH family protein -
  LEN_RS04630 (LEN_0952) - 1087373..1088743 (+) 1371 WP_096376850.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41055.33 Da        Isoelectric Point: 6.1908

>NTDB_id=67447 LEN_RS04610 WP_096376847.1 1083734..1084849(-) (pilU) [Lysobacter enzymogenes strain M497-1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKSVIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSNTAGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLKAVISQRLVVGTDGRRIPAAEVLINTPHIRDLMRRGQVHEIKQAMEESLEDGMESFDQCL
FRLYKEGKIEMEEALKAADSRDGLALKFRLSEGGTGEHDPYADMYDASATH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=67447 LEN_RS04610 WP_096376847.1 1083734..1084849(-) (pilU) [Lysobacter enzymogenes strain M497-1]
ATGGACATCGGCTATTTCTTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTTCTGACCACTGGCGCGCCGGTCTA
CATCAAAGTCGAGGGGCGCCTGTACCCGCTCGGCAACACCGGCTTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGACCTCGAACTCAACATGGCCCTGGCCCTGCCCGACGCGGGGCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATTCCCAGCATCGA
GGAATTGCAGCTGCCGCAGGTGCTCAAGAGCGTCATCATGGCGCCGCGCGGGCTGGTGCTGATCGTCGGCTCGACCGGCT
CGGGCAAGTCGACCACGCTGGCCTCTATGATCGACCACCGCAACAGCAACACCGCCGGTCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTCGACGCCACCACGATGGAGGCGG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCCTCGAGCGCATC
CTCAATTTCTTCCCCGAGGCCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTCAAGGCGGTGATTTCGCAGCG
GCTGGTGGTCGGCACCGACGGCCGCCGCATCCCGGCCGCGGAAGTGCTGATCAACACTCCGCACATCCGCGACCTGATGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAAGAGTCGCTGGAAGACGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGGCTGTACAAGGAAGGCAAGATCGAGATGGAGGAGGCGCTCAAGGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGCACCGGCGAGCACGATCCTTACGCGGACATGTACGACGCCTCGGCTACGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

96.765

0.496
  pilU Acinetobacter baylyi ADP1

49.435

95.418

0.472
  pilU Vibrio cholerae strain A1552

48.87

95.418

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.428

91.375

0.415
  pilT Pseudomonas aeruginosa PAK

42.012

91.105

0.383
  pilT Acinetobacter baumannii D1279779

41.124

91.105

0.375
  pilT Acinetobacter baumannii strain A118

41.124

91.105

0.375
  pilT Acinetobacter nosocomialis M2

41.124

91.105

0.375
  pilT Acinetobacter baylyi ADP1

40.588

91.644

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.533

91.105

0.369
  pilT Legionella pneumophila strain Lp02

39.941

91.105

0.364
  pilT Legionella pneumophila strain ERS1305867

39.941

91.105

0.364
  pilT Neisseria gonorrhoeae MS11

38.617

93.531

0.361
  pilT Neisseria meningitidis 8013

38.617

93.531

0.361

Multiple sequence alignment