Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MTP21_RS12300 Genome accession   NZ_CP094880
Coordinates   2710699..2711805 (-) Length   368 a.a.
NCBI ID   WP_014233031.1    Uniprot ID   A0AAN0Y4F4
Organism   Vibrio natriegens strain WPAGA4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2705699..2716805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTP21_RS12270 - 2706307..2706804 (+) 498 WP_239948329.1 SprT family zinc-dependent metalloprotease -
  MTP21_RS12275 endA 2706936..2707631 (+) 696 WP_239948328.1 deoxyribonuclease I -
  MTP21_RS12280 rsmE 2707771..2708502 (+) 732 WP_065302893.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MTP21_RS12285 gshB 2708517..2709467 (+) 951 WP_014233028.1 glutathione synthase -
  MTP21_RS12290 - 2709603..2710166 (+) 564 WP_014233029.1 YqgE/AlgH family protein -
  MTP21_RS12295 ruvX 2710208..2710633 (+) 426 WP_014233030.1 Holliday junction resolvase RuvX -
  MTP21_RS12300 pilU 2710699..2711805 (-) 1107 WP_014233031.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MTP21_RS12305 pilT 2711836..2712876 (-) 1041 WP_014233032.1 type IV pilus twitching motility protein PilT Machinery gene
  MTP21_RS12310 - 2712905..2713618 (+) 714 WP_243974905.1 YggS family pyridoxal phosphate-dependent enzyme -
  MTP21_RS12315 proC 2713741..2714559 (+) 819 WP_065297395.1 pyrroline-5-carboxylate reductase -
  MTP21_RS12320 - 2714612..2715169 (+) 558 WP_014233035.1 YggT family protein -
  MTP21_RS12325 yggU 2715169..2715459 (+) 291 WP_118119396.1 DUF167 family protein YggU -
  MTP21_RS12330 - 2715534..2715965 (+) 432 WP_020334002.1 DUF4426 domain-containing protein -
  MTP21_RS12335 - 2716099..2716701 (+) 603 WP_118119398.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41300.35 Da        Isoelectric Point: 6.2478

>NTDB_id=673898 MTP21_RS12300 WP_014233031.1 2710699..2711805(-) (pilU) [Vibrio natriegens strain WPAGA4]
MDLNKFLEGMLALQASDLYITVGAPILFRVDGELRPQGDKLTENDVAQLLDNAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETDIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKSGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPLATGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=673898 MTP21_RS12300 WP_014233031.1 2710699..2711805(-) (pilU) [Vibrio natriegens strain WPAGA4]
ATGGATCTGAATAAATTTCTTGAAGGTATGCTGGCGCTGCAAGCGTCGGATCTTTATATCACGGTCGGCGCGCCGATTTT
GTTTCGTGTCGACGGCGAGTTACGTCCGCAAGGTGACAAGCTGACAGAAAATGATGTGGCGCAGTTGCTGGATAATGCAA
TGGATCCTGAGCGTCGTCAGGAGTTTCGAAAAAGTCGAGAGTCTAACTTTGCTATCGTCAGAGACTGTGGTCGATTCCGT
GTCAGTGCCTTTTTCCAGCGAGAATTGCCGGGAGCGGTTATTCGCCGTATTGAGACCGACATCCCTACCTTTGAGCAGCT
CAAACTGCCTCTGGTGTTGCAAGATTTAGCGATCGCCAAGCGAGGTTTGGTGCTGGTGGTTGGTGCGACTGGCTCTGGTA
AGTCGACCACAATGGCGGCGATGACAGGCTACCGAAACAGTAATAAGACCGGGCATATCCTGACGGTAGAAGATCCGATC
GAATTTGTTCATGAACACCAGCGTTGTATCGTAACCCAGCGTGAAGTCGGTTTAGACACAGAAAGCTATGAAGTGGCACT
GAAAAACTCCCTGCGACAGGCTCCGGATATGATTTTGATCGGCGAAATCCGCAGTAGAGAGACCATGGAGTATGCGATGA
CCTTTGCTGAAACCGGGCATTTGTGTATGGCGACTCTCCACGCGAATAACGCCAACCAGGCATTAGAGCGGATTCTGCAT
TTAGTACCTAAAGATCAAAAAGAGCAGTTCTTGTTTGATCTGTCTATGAACTTAAAAGGCGTGGTTGGGCAGCAACTGAT
CCGCGATAAAAGTGGGCAGGGACGTCATGGCGTATTTGAGATCCTGCTGAACAGCCCGCGTGTTTCGGATTTAATCCGTC
GTGGCGATCTGCATGAACTTAAGTCGACGATGGCTCGTTCAAATGAGTTTGGTATGCTGACCTTTGACCAGTCGCTCTAC
AAGTTAGTGATGCAAGGCAAAATCAGTGAAGAAGATGCACTGCACAGTGCTGATTCGGCAAATGACCTGCGTCTCATGCT
GAAAACTCAACGCGGTGAGCCTCTGGCAACAGGAAGCTTGGCGAACGTTAAGATCGATATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.522

100

0.815
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44

88.315

0.389
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Acinetobacter baylyi ADP1

40.988

93.478

0.383
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

40.351

92.935

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372