Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GM3709_RS03000 Genome accession   NZ_AP014821
Coordinates   715321..716439 (+) Length   372 a.a.
NCBI ID   WP_066116149.1    Uniprot ID   A0A0D6AM30
Organism   Geminocystis sp. NIES-3709     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 710321..721439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM3709_RS02980 (GM3709_623) - 710808..710990 (+) 183 WP_066116142.1 hypothetical protein -
  GM3709_RS02985 (GM3709_624) argH 711233..712627 (+) 1395 WP_066116143.1 argininosuccinate lyase -
  GM3709_RS02990 (GM3709_625) - 712905..713882 (+) 978 WP_066116145.1 hypothetical protein -
  GM3709_RS02995 (GM3709_626) - 714031..715020 (+) 990 WP_066116147.1 hypothetical protein -
  GM3709_RS03000 (GM3709_627) pilT 715321..716439 (+) 1119 WP_066116149.1 type IV pilus twitching motility protein PilT Machinery gene
  GM3709_RS03005 (GM3709_628) - 716531..717745 (+) 1215 WP_066116151.1 type II secretion system F family protein -
  GM3709_RS03010 (GM3709_629) - 717865..718440 (+) 576 WP_066116154.1 hypothetical protein -
  GM3709_RS03015 (GM3709_630) - 718543..720027 (-) 1485 WP_066116155.1 protein kinase domain-containing protein -
  GM3709_RS03020 (GM3709_631) - 720067..720354 (-) 288 WP_066116157.1 hypothetical protein -
  GM3709_RS03025 (GM3709_632) - 720623..720844 (+) 222 WP_066116159.1 DUF167 domain-containing protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40636.01 Da        Isoelectric Point: 6.3641

>NTDB_id=66938 GM3709_RS03000 WP_066116149.1 715321..716439(+) (pilT) [Geminocystis sp. NIES-3709]
MELMIEDLMEQLVELGGSDMHIQAGAPIYFRISGKLTPIGEEPLNPQECQKLIFSMLNNTQRKTLEQEWELDCSYGVKGL
ARFRVNVYKERGCYAACLRALSSKIPNFDQLGLPDIVKEMTDRPRGLILVTGQTGSGKTTTLAAMLDLINRTRAEHILTV
EDPIEYVFPNINSLFHQRQKGEDTKSFGNALKAALREDPDIILVGEMRDLETIALAISAAETGHLVFGTLHTSSAAGTID
RIIDVFPAAEQAQIRAMLSNSLLAVFAQTLAKKKNPKPGEFGRAMAQEIMIITPAIANLIREAKAAQIYSAIQTGGKLGM
QTMEQALAGYVKTGIISIEEGLAKSSKPDELKRLLAGSGIDADMAMTGGKRR

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=66938 GM3709_RS03000 WP_066116149.1 715321..716439(+) (pilT) [Geminocystis sp. NIES-3709]
ATGGAATTAATGATCGAAGATTTGATGGAACAGTTGGTAGAATTGGGTGGCTCAGATATGCACATTCAGGCAGGAGCACC
GATTTACTTTCGTATCAGCGGTAAATTGACTCCCATCGGTGAAGAGCCTTTAAATCCTCAAGAGTGTCAAAAATTAATCT
TTAGTATGTTGAATAACACTCAACGGAAAACTCTCGAACAGGAATGGGAGTTGGATTGTTCTTACGGTGTTAAGGGTTTA
GCTCGATTTCGGGTTAATGTTTATAAAGAAAGAGGTTGTTATGCGGCTTGTTTACGCGCGTTATCTTCTAAAATCCCTAA
CTTCGATCAATTGGGTTTACCTGATATTGTCAAGGAAATGACCGATCGACCTAGAGGTCTTATTCTTGTAACTGGTCAAA
CAGGTTCAGGCAAAACTACGACTTTAGCGGCAATGTTGGATTTAATTAATCGTACTCGTGCTGAACATATCTTAACAGTT
GAAGACCCGATCGAGTATGTATTTCCCAATATTAATAGCTTATTTCATCAGCGACAAAAAGGAGAAGATACCAAAAGTTT
TGGTAATGCGTTAAAAGCGGCACTGCGGGAAGATCCTGACATTATTCTGGTGGGGGAAATGCGAGATTTGGAAACTATTG
CTTTGGCGATTAGTGCGGCGGAAACGGGACACCTCGTATTTGGGACTCTCCATACCAGTTCGGCGGCAGGTACAATTGAT
CGTATTATTGATGTATTTCCAGCGGCAGAACAAGCACAAATTCGGGCAATGTTATCTAACTCTTTATTAGCGGTATTCGC
TCAAACCTTGGCGAAAAAGAAAAACCCTAAACCGGGAGAATTTGGACGAGCAATGGCTCAAGAAATCATGATTATTACCC
CTGCGATCGCTAACTTAATACGGGAAGCAAAAGCCGCCCAGATTTACTCAGCTATTCAAACAGGAGGAAAATTAGGAATG
CAAACTATGGAACAAGCCTTAGCAGGGTATGTTAAAACAGGAATTATTAGCATAGAAGAAGGATTAGCCAAGAGTAGTAA
ACCTGATGAATTAAAACGTTTGTTAGCAGGTAGTGGCATTGATGCAGATATGGCAATGACAGGTGGTAAAAGACGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6AM30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.026

93.28

0.495
  pilT Legionella pneumophila strain ERS1305867

54.655

89.516

0.489
  pilT Legionella pneumophila strain Lp02

54.655

89.516

0.489
  pilT Acinetobacter baumannii D1279779

52.161

93.28

0.487
  pilT Acinetobacter baumannii strain A118

52.161

93.28

0.487
  pilT Acinetobacter nosocomialis M2

52.161

93.28

0.487
  pilT Acinetobacter baylyi ADP1

51.873

93.28

0.484
  pilT Pseudomonas stutzeri DSM 10701

51.297

93.28

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.147

91.667

0.46
  pilT Vibrio cholerae strain A1552

50.147

91.667

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.714

94.086

0.449
  pilT Neisseria gonorrhoeae MS11

47.774

90.591

0.433
  pilT Neisseria meningitidis 8013

47.774

90.591

0.433
  pilU Pseudomonas stutzeri DSM 10701

37.883

96.505

0.366

Multiple sequence alignment