Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GM3708_RS14500 Genome accession   NZ_AP014815
Coordinates   3281665..3282771 (+) Length   368 a.a.
NCBI ID   WP_066349502.1    Uniprot ID   A0A0D6AIF6
Organism   Geminocystis sp. NIES-3708     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3276665..3287771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM3708_RS14480 (GM3708_2989) - 3277074..3277289 (-) 216 WP_066348433.1 hypothetical protein -
  GM3708_RS14485 (GM3708_2990) - 3277458..3278822 (-) 1365 WP_315862629.1 TolC family protein -
  GM3708_RS14490 (GM3708_2991) - 3279221..3280810 (+) 1590 WP_066348437.1 glycoside hydrolase family 57 protein -
  GM3708_RS14495 (GM3708_2992) - 3280898..3281350 (+) 453 WP_082714142.1 DUF2996 domain-containing protein -
  GM3708_RS14500 (GM3708_2993) pilT 3281665..3282771 (+) 1107 WP_066349502.1 type IV pilus twitching motility protein PilT Machinery gene
  GM3708_RS14505 (GM3708_2994) - 3282839..3284053 (+) 1215 WP_066348441.1 type II secretion system F family protein -
  GM3708_RS14510 (GM3708_2995) - 3284094..3284666 (+) 573 WP_066348443.1 hypothetical protein -
  GM3708_RS14515 (GM3708_2996) - 3284808..3285353 (-) 546 WP_066348448.1 TolB family protein -
  GM3708_RS14520 (GM3708_2997) - 3285414..3286217 (-) 804 WP_066348451.1 Ycf66 family protein -
  GM3708_RS14525 (GM3708_2998) psbX 3286380..3286496 (-) 117 WP_066348453.1 photosystem II reaction center X protein -
  GM3708_RS14530 (GM3708_2999) - 3286588..3286887 (+) 300 WP_066348454.1 YggT family protein -
  GM3708_RS14535 (GM3708_3000) folB 3287003..3287359 (+) 357 WP_066348457.1 dihydroneopterin aldolase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40195.44 Da        Isoelectric Point: 6.6490

>NTDB_id=66933 GM3708_RS14500 WP_066349502.1 3281665..3282771(+) (pilT) [Geminocystis sp. NIES-3708]
MELMIEDLMEQLVELGGSDMHIQAGAPVYFRISGKLTPIGEDPLTPQECQKLIFSMLNNTQRKTLEQEWELDCSYGVKGL
ARFRVNVYKERGCYAACLRALSSKIPNFDQLGLPDIVKEMTDRPRGLILVTGPTGSGKTTTLAAMLDLINRTRAEHILTV
EDPIEYVFPNVNSLFHQRQKGEDTKSFANALKAALREDPDIILVGEMRDLETISLAVSAAETGHLVFGTLHTSSAAGTID
RIIDVFPSAEQGQIRAMLSNSLLAVFAQTLAKKKNPKPGEFGRAMAQEIMIVTPAIANLIREAKAPQIYSAIQTGGKLGM
QTMEQALAGYVKTGTISLEEGLAKSSKPDELKRLLVGSGMEGTATKKR

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=66933 GM3708_RS14500 WP_066349502.1 3281665..3282771(+) (pilT) [Geminocystis sp. NIES-3708]
ATGGAATTAATGATCGAAGACTTAATGGAGCAGTTAGTCGAATTAGGTGGCTCTGATATGCACATTCAAGCAGGGGCACC
TGTTTATTTTCGCATCAGTGGTAAACTTACTCCTATTGGTGAAGATCCATTGACTCCTCAAGAGTGTCAAAAACTAATTT
TTAGTATGTTAAATAACACTCAGCGTAAAACCCTTGAGCAAGAATGGGAGCTAGATTGTTCTTATGGAGTTAAAGGTTTA
GCTCGATTTCGGGTTAATGTCTATAAAGAAAGAGGTTGTTATGCGGCTTGTTTAAGAGCTTTATCTTCTAAAATTCCTAA
CTTTGATCAATTGGGTTTACCTGATATTGTCAAGGAAATGACTGATCGTCCTCGAGGTTTAATTCTTGTAACAGGACCTA
CTGGATCTGGTAAAACAACAACTTTAGCCGCAATGTTAGATTTAATTAATCGTACTCGTGCCGAACATATCTTAACTGTA
GAAGATCCCATTGAGTATGTATTTCCTAATGTTAATAGCTTATTTCATCAACGACAAAAAGGAGAAGATACAAAAAGTTT
TGCTAATGCATTAAAAGCGGCTTTAAGGGAAGACCCTGATATTATTCTGGTGGGTGAGATGCGAGATTTAGAAACTATCT
CTCTCGCTGTTAGTGCGGCGGAAACAGGACACCTTGTATTCGGAACTCTTCACACTAGTTCAGCAGCTGGTACGATAGAT
CGTATTATTGATGTTTTTCCCTCCGCAGAACAAGGACAAATTCGGGCAATGTTATCTAACTCTTTACTAGCCGTATTTGC
TCAAACTTTGGCTAAGAAGAAAAATCCCAAACCTGGTGAATTTGGACGAGCGATGGCTCAAGAAATTATGATTGTTACTC
CAGCGATCGCTAACTTGATTCGGGAAGCAAAAGCACCTCAGATTTACTCAGCGATTCAAACAGGAGGTAAATTAGGAATG
CAAACCATGGAACAAGCCTTAGCAGGTTACGTAAAAACGGGAACAATTAGTTTAGAAGAAGGATTAGCTAAAAGCAGTAA
ACCCGATGAATTAAAACGTTTATTAGTTGGTAGTGGTATGGAAGGTACTGCAACAAAAAAAAGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6AIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.45

94.293

0.495
  pilT Legionella pneumophila strain ERS1305867

54.054

90.489

0.489
  pilT Legionella pneumophila strain Lp02

54.054

90.489

0.489
  pilT Acinetobacter baumannii D1279779

51.873

94.293

0.489
  pilT Acinetobacter baumannii strain A118

51.873

94.293

0.489
  pilT Acinetobacter nosocomialis M2

51.873

94.293

0.489
  pilT Acinetobacter baylyi ADP1

51.585

94.293

0.486
  pilT Pseudomonas stutzeri DSM 10701

51.009

94.293

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.733

92.663

0.47
  pilT Vibrio cholerae strain A1552

50.733

92.663

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48

95.109

0.457
  pilT Neisseria meningitidis 8013

48.071

91.576

0.44
  pilT Neisseria gonorrhoeae MS11

48.071

91.576

0.44
  pilU Pseudomonas stutzeri DSM 10701

38.095

97.011

0.37

Multiple sequence alignment