Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   MP387_RS00825 Genome accession   NZ_CP094226
Coordinates   163482..164423 (+) Length   313 a.a.
NCBI ID   WP_242746934.1    Uniprot ID   -
Organism   Streptococcus oralis strain 1648     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 158482..169423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MP387_RS00805 (MP387_00805) tgt 158515..159657 (+) 1143 WP_001285249.1 tRNA guanosine(34) transglycosylase Tgt -
  MP387_RS00810 (MP387_00810) - 159791..161608 (+) 1818 WP_242746929.1 acyltransferase family protein -
  MP387_RS00815 (MP387_00815) nagA 161749..162900 (+) 1152 WP_001134442.1 N-acetylglucosamine-6-phosphate deacetylase -
  MP387_RS00820 (MP387_00820) - 163053..163418 (+) 366 WP_000286396.1 DUF1033 family protein -
  MP387_RS00825 (MP387_00825) comGA/cglA/cilD 163482..164423 (+) 942 WP_242746934.1 competence type IV pilus ATPase ComGA Machinery gene
  MP387_RS00830 (MP387_00830) comGB/cglB 164371..165387 (+) 1017 WP_242748017.1 competence type IV pilus assembly protein ComGB Machinery gene
  MP387_RS00835 (MP387_00835) comGC/cglC 165389..165712 (+) 324 WP_000735798.1 competence type IV pilus major pilin ComGC Machinery gene
  MP387_RS00840 (MP387_00840) comGD/cglD 165675..166109 (+) 435 WP_242746960.1 competence type IV pilus minor pilin ComGD Machinery gene
  MP387_RS00845 (MP387_00845) comGE/cglE 166072..166374 (+) 303 WP_242746963.1 competence type IV pilus minor pilin ComGE Machinery gene
  MP387_RS00850 (MP387_00850) comGF/cglF 166337..166798 (+) 462 WP_242746966.1 competence type IV pilus minor pilin ComGF Machinery gene
  MP387_RS00855 (MP387_00855) comGG/cglG 166776..167189 (+) 414 WP_242746969.1 competence type IV pilus minor pilin ComGG Machinery gene
  MP387_RS00860 (MP387_00860) - 167222..167812 (+) 591 WP_242746972.1 class I SAM-dependent methyltransferase -
  MP387_RS00865 (MP387_00865) comYH 167871..168824 (+) 954 WP_242746975.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35541.50 Da        Isoelectric Point: 5.2775

>NTDB_id=668074 MP387_RS00825 WP_242746934.1 163482..164423(+) (comGA/cglA/cilD) [Streptococcus oralis strain 1648]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDILAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKVSSLRLSTVGDYRGHESLVIRLLHDEEKDLQFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFMGQQ
VMSIEDPVEIKQEDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGILDFANKDYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=668074 MP387_RS00825 WP_242746934.1 163482..164423(+) (comGA/cglA/cilD) [Streptococcus oralis strain 1648]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGAAAAGAAGGCCCAGGATATCTATTTTATCCCCAAGGA
AAAGTCCTATGAGCTTCACATGCGGGTTGGAGACGAGCGGTGTCTCGTTGACTCCTATGAGTTTGATATTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTAGCGGGTATGAATGTAGGAGAGAAGAGACGTAGTCAGCTGGGATCTTGCGACTATCAG
CATGGGGAGAAGGTGTCTTCTCTGCGTTTGTCTACCGTGGGAGATTATCGGGGGCATGAGAGTTTGGTCATTCGTTTGTT
GCACGATGAGGAGAAGGACCTGCAATTCTGGTTTCAGGATATGAACGAACTGGGTGAACAGTACAGGCAACGGGGCCTCT
ATCTCTTTGCTGGCCCAGTCGGCAGTGGCAAGACGACTCTGATGCATGAATTAGCCAAGTCTCTGTTTATGGGGCAACAG
GTCATGTCCATTGAAGATCCTGTCGAGATCAAGCAGGAAGACATGCTCCAGTTGCAGTTAAATGAAGCGATTGGCTTGAC
CTATGAAAATCTGATCAAACTGTCTCTCCGGCATCGTCCGGACCTCTTGATTATTGGTGAAATTCGGGATAGTGAGACGG
CGCGTGCGGTTGTCAGAGCCAGTTTGACAGGGGCGACAGTCTTTTCAACTATTCATGCCAAGAGTATCCGAGGTGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAACTAGCAGTTGTCCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCCTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAGCAGATTGATCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

88.498

100

0.885
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

87.54

100

0.875
  comGA/cglA/cilD Streptococcus pneumoniae D39

87.54

100

0.875
  comGA/cglA/cilD Streptococcus pneumoniae R6

87.54

100

0.875
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

87.54

100

0.875
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.559

99.361

0.661
  comYA Streptococcus mutans UA140

66.559

99.361

0.661
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.645

99.042

0.601
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

43.346

84.026

0.364

Multiple sequence alignment