Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGF/cglF   Type   Machinery gene
Locus tag   MP387_RS00850 Genome accession   NZ_CP094226
Coordinates   166337..166798 (+) Length   153 a.a.
NCBI ID   WP_242746966.1    Uniprot ID   -
Organism   Streptococcus oralis strain 1648     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 161337..171798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MP387_RS00815 (MP387_00815) nagA 161749..162900 (+) 1152 WP_001134442.1 N-acetylglucosamine-6-phosphate deacetylase -
  MP387_RS00820 (MP387_00820) - 163053..163418 (+) 366 WP_000286396.1 DUF1033 family protein -
  MP387_RS00825 (MP387_00825) comGA/cglA/cilD 163482..164423 (+) 942 WP_242746934.1 competence type IV pilus ATPase ComGA Machinery gene
  MP387_RS00830 (MP387_00830) comGB/cglB 164371..165387 (+) 1017 WP_242748017.1 competence type IV pilus assembly protein ComGB Machinery gene
  MP387_RS00835 (MP387_00835) comGC/cglC 165389..165712 (+) 324 WP_000735798.1 competence type IV pilus major pilin ComGC Machinery gene
  MP387_RS00840 (MP387_00840) comGD/cglD 165675..166109 (+) 435 WP_242746960.1 competence type IV pilus minor pilin ComGD Machinery gene
  MP387_RS00845 (MP387_00845) comGE/cglE 166072..166374 (+) 303 WP_242746963.1 competence type IV pilus minor pilin ComGE Machinery gene
  MP387_RS00850 (MP387_00850) comGF/cglF 166337..166798 (+) 462 WP_242746966.1 competence type IV pilus minor pilin ComGF Machinery gene
  MP387_RS00855 (MP387_00855) comGG/cglG 166776..167189 (+) 414 WP_242746969.1 competence type IV pilus minor pilin ComGG Machinery gene
  MP387_RS00860 (MP387_00860) - 167222..167812 (+) 591 WP_242746972.1 class I SAM-dependent methyltransferase -
  MP387_RS00865 (MP387_00865) comYH 167871..168824 (+) 954 WP_242746975.1 class I SAM-dependent methyltransferase Machinery gene
  MP387_RS00870 (MP387_00870) - 168875..170065 (+) 1191 WP_242746978.1 acetate kinase -
  MP387_RS00875 (MP387_00875) rnpA 170215..170586 (+) 372 WP_000748120.1 ribonuclease P protein component -
  MP387_RS00880 (MP387_00880) - 170561..171388 (+) 828 WP_242746981.1 membrane protein insertase YidC -

Sequence


Protein


Download         Length: 153 a.a.        Molecular weight: 17803.47 Da        Isoelectric Point: 9.6234

>NTDB_id=668079 MP387_RS00850 WP_242746966.1 166337..166798(+) (comGF/cglF) [Streptococcus oralis strain 1648]
MVQRSCSTLKSSKVRAFTLLESLIALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELERSQFEKVEAN
RLYMKQDGKDVSIGKSKSDDFRKTDTSGRGYQPMVYGLKSAQITEENQLVRFRFQFQKGLEREFIYRVEKAKS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=668079 MP387_RS00850 WP_242746966.1 166337..166798(+) (comGF/cglF) [Streptococcus oralis strain 1648]
ATGGTTCAGAGAAGTTGCTCGACCTTAAAGAGCAGTAAGGTAAGAGCGTTCACTCTATTAGAATCTCTGATTGCCCTTAT
CGTCATTAGCGGAGGCTTGCTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGTTACCAGCAGCAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTGGAGGTAGAGTTAGAGCGTTCGCAGTTTGAAAAGGTGGAAGCCAAT
CGCCTCTACATGAAACAGGATGGTAAGGATGTATCTATAGGGAAGTCTAAATCGGATGATTTTCGGAAGACCGATACCAG
CGGACGGGGCTACCAACCTATGGTTTATGGACTCAAATCAGCACAGATTACAGAGGAAAATCAATTAGTTCGCTTTCGTT
TCCAATTTCAAAAGGGTTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGCAAAAAGTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus mitis SK321

89.542

100

0.895

  comGF/cglF Streptococcus mitis NCTC 12261

88.235

100

0.882

  comGF/cglF Streptococcus pneumoniae Rx1

87.582

100

0.876

  comGF/cglF Streptococcus pneumoniae D39

87.582

100

0.876

  comGF/cglF Streptococcus pneumoniae R6

87.582

100

0.876

  comGF/cglF Streptococcus pneumoniae TIGR4

87.582

100

0.876

  comGF Lactococcus lactis subsp. cremoris KW2

40.714

91.503

0.373


Multiple sequence alignment