Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MOP87_RS19690 Genome accession   NZ_CP093940
Coordinates   4397668..4398663 (-) Length   331 a.a.
NCBI ID   WP_027913501.1    Uniprot ID   -
Organism   Pseudomonas sp. I3-I5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4392668..4403663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOP87_RS19670 (MOP87_19670) argS 4393885..4395621 (+) 1737 WP_027913503.1 arginine--tRNA ligase -
  MOP87_RS19675 (MOP87_19675) - 4395623..4396321 (+) 699 WP_023532748.1 SPOR domain-containing protein -
  MOP87_RS19680 (MOP87_19680) - 4396378..4396797 (-) 420 WP_023532338.1 TM2 domain-containing protein -
  MOP87_RS19685 (MOP87_19685) - 4396964..4397593 (+) 630 WP_027913502.1 C40 family peptidase -
  MOP87_RS19690 (MOP87_19690) pilT 4397668..4398663 (-) 996 WP_027913501.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MOP87_RS19695 (MOP87_19695) - 4398728..4399414 (+) 687 WP_027913500.1 YggS family pyridoxal phosphate-dependent enzyme -
  MOP87_RS19700 (MOP87_19700) proC 4399453..4400271 (+) 819 WP_242809910.1 pyrroline-5-carboxylate reductase -
  MOP87_RS19705 (MOP87_19705) - 4400282..4400872 (+) 591 WP_023532686.1 YggT family protein -
  MOP87_RS19710 (MOP87_19710) - 4400961..4402100 (+) 1140 WP_028686690.1 homoserine O-acetyltransferase -
  MOP87_RS19715 (MOP87_19715) metW 4402108..4402728 (+) 621 WP_023532733.1 methionine biosynthesis protein MetW -
  MOP87_RS19720 (MOP87_19720) - 4402747..4403181 (+) 435 WP_242809911.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 35538.21 Da        Isoelectric Point: 7.5174

>NTDB_id=665760 MOP87_RS19690 WP_027913501.1 4397668..4398663(-) (pilT) [Pseudomonas sp. I3-I5]
MEVTDLLALALKAGASDMHLAAGQAPAMRVHGVLQRLPVPMATPDELMRSMESLLDADPYRQWAAGHDLDVALELPALGR
FRVNLFRQMNGPALTVRLIPARIACAEALGLGEIVRTTGRYRDGLVLIGGPTGSGKTSTLAALLDQMGRERALHIVTLED
PIEVVHASHQGVVNQREIGRDCCSFAQGLRSALRQDPDVIVLGELRDLETIRLALRAAETGHLVLATVHTRSAVNSVDRV
VEVFPAQEKPWVRAMLADSLRVVVAQVLVGRRGGGRVAAREVLVVNDAARNLIREGRMAQLHSVMQTGAAEGMQTLAGAM
KQLQAGGLLAE

Nucleotide


Download         Length: 996 bp        

>NTDB_id=665760 MOP87_RS19690 WP_027913501.1 4397668..4398663(-) (pilT) [Pseudomonas sp. I3-I5]
ATGGAGGTGACAGACCTGCTGGCGCTTGCCTTGAAGGCAGGTGCCAGTGACATGCACCTGGCTGCAGGTCAGGCACCGGC
GATGCGCGTGCATGGCGTGCTGCAACGCCTGCCGGTACCGATGGCAACGCCGGATGAATTGATGCGAAGCATGGAATCGT
TGCTCGATGCCGACCCGTATCGACAGTGGGCTGCCGGTCACGACCTGGACGTGGCACTGGAGCTGCCCGCGCTCGGGCGG
TTTCGCGTGAACCTGTTCAGGCAGATGAACGGCCCCGCGCTCACTGTCCGCTTGATCCCCGCGCGCATCGCCTGCGCTGA
GGCGCTTGGCCTGGGCGAGATCGTCCGAACCACTGGCCGATACCGTGACGGTCTGGTCCTCATAGGCGGGCCGACGGGCA
GTGGCAAGACGAGCACATTGGCAGCCCTGCTCGATCAGATGGGCCGCGAGCGTGCGCTGCACATCGTCACCCTCGAGGAT
CCGATCGAGGTCGTCCATGCCAGCCATCAAGGTGTGGTCAACCAGCGCGAAATCGGCCGCGATTGCTGCAGCTTTGCTCA
AGGCCTGCGCAGCGCATTGCGCCAGGATCCCGACGTGATCGTGCTGGGCGAGTTGAGGGACCTGGAGACCATTCGTCTTG
CACTGCGTGCCGCCGAAACGGGTCATCTGGTACTGGCAACCGTTCATACACGGTCAGCGGTGAACAGTGTCGACCGGGTG
GTGGAGGTATTCCCAGCGCAGGAAAAGCCTTGGGTGCGCGCGATGCTGGCGGACTCGCTGCGAGTGGTTGTGGCGCAAGT
GCTGGTCGGCCGGCGGGGCGGGGGCAGGGTCGCGGCGCGAGAAGTGCTGGTGGTGAACGATGCGGCCCGCAACCTGATCC
GCGAAGGGCGCATGGCCCAGCTTCATTCGGTGATGCAGACCGGCGCGGCAGAGGGCATGCAAACCCTGGCCGGGGCCATG
AAGCAACTTCAAGCGGGCGGGCTGCTCGCCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

49.091

99.698

0.489
  pilT Acinetobacter baumannii D1279779

49.091

99.698

0.489
  pilT Acinetobacter baylyi ADP1

49.24

99.396

0.489
  pilT Acinetobacter nosocomialis M2

48.788

99.698

0.486
  pilT Pseudomonas stutzeri DSM 10701

48.485

99.698

0.483
  pilT Pseudomonas aeruginosa PAK

48.485

99.698

0.483
  pilT Vibrio cholerae strain A1552

48.024

99.396

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.024

99.396

0.477
  pilT Neisseria meningitidis 8013

46.828

100

0.468
  pilT Neisseria gonorrhoeae MS11

46.526

100

0.465
  pilT Legionella pneumophila strain Lp02

46.224

100

0.462
  pilT Legionella pneumophila strain ERS1305867

46.224

100

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

95.77

0.426
  pilU Pseudomonas stutzeri DSM 10701

37.462

100

0.375