Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MNR01_RS14160 Genome accession   NZ_CP093341
Coordinates   3156570..3157682 (-) Length   370 a.a.
NCBI ID   WP_241918407.1    Uniprot ID   -
Organism   Lysobacter sp. S4-A87     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3151570..3162682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNR01_RS14125 (MNR01_14125) coq7 3151610..3152254 (-) 645 WP_241918402.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  MNR01_RS14130 (MNR01_14130) - 3152264..3153457 (-) 1194 WP_241918403.1 (Fe-S)-binding protein -
  MNR01_RS14135 (MNR01_14135) rplM 3153624..3154052 (+) 429 WP_158733500.1 50S ribosomal protein L13 -
  MNR01_RS14140 (MNR01_14140) rpsI 3154063..3154455 (+) 393 WP_241918404.1 30S ribosomal protein S9 -
  MNR01_RS14150 (MNR01_14150) - 3154793..3155776 (+) 984 WP_241918405.1 fumarylacetoacetate hydrolase family protein -
  MNR01_RS14155 (MNR01_14155) maiA 3155853..3156524 (+) 672 WP_241918406.1 maleylacetoacetate isomerase -
  MNR01_RS14160 (MNR01_14160) pilU 3156570..3157682 (-) 1113 WP_241918407.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MNR01_RS14165 (MNR01_14165) - 3157871..3158170 (+) 300 WP_241918408.1 DUF4398 domain-containing protein -
  MNR01_RS14170 (MNR01_14170) - 3158167..3159048 (+) 882 WP_241918409.1 OmpA family protein -
  MNR01_RS14175 (MNR01_14175) - 3159497..3159715 (+) 219 WP_158733494.1 YdcH family protein -
  MNR01_RS14180 (MNR01_14180) - 3159859..3161235 (+) 1377 WP_241918410.1 pyridoxal-phosphate dependent enzyme -
  MNR01_RS14185 (MNR01_14185) - 3161232..3162176 (+) 945 WP_241918411.1 2OG-Fe(II) oxygenase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41054.37 Da        Isoelectric Point: 6.0938

>NTDB_id=663828 MNR01_RS14160 WP_241918407.1 3156570..3157682(-) (pilU) [Lysobacter sp. S4-A87]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPLFERDLELNMALALPEAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDYRNSNISGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLRAVVSQRLVVGSDGRRLPASEVLINTPHVRDLMRRGQVHEIKQAMEASLEEGMESFDQCL
FRLHKEGRIDMEEALKAADSREGLSLKFRLSEGATGEHDPYAEVYDAVTQ

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=663828 MNR01_RS14160 WP_241918407.1 3156570..3157682(-) (pilU) [Lysobacter sp. S4-A87]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAGAATGCGTCAGACATGTTCCTGACGACCGGCGCGCCGGTGTA
CATCAAGGTCGAAGGCCGCCTGTACCCGCTGGGCAATACCGGCCTGCCTCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAGGGGCAGGTGCCGCTGTTCGAGCGGGACCTCGAACTCAACATGGCCCTGGCCCTGCCCGAAGCCGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGTGCCATCCGCAGCGTGATTCCCTCGATCGA
GGAATTGCAGCTGCCGCAGGTGCTCAAGGACATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGTCGATGATCGACTACCGCAACAGCAATATCTCGGGGCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAAAAGTCGATCGTCAACCAGCGCGAAGTCGGCCTCGACACGCACGCCTTCCACAA
CGCGCTCAAGAACGCGATGCGTGAAGCACCGGACGTGATCCTGATCGGCGAAATCCTCGACGCGACCACGATGGAAGCGG
CGATCGCCTTCGCCGAAACCGGTCACCTTTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACGCTCGAGCGCATC
CTCAACTTCTTCCCCGAGGCTGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTCAGCCAGCG
CCTGGTGGTCGGCAGCGACGGCCGCCGCCTGCCGGCGTCGGAAGTGCTGATCAACACCCCGCACGTGCGCGACCTGATGC
GCCGTGGCCAGGTGCACGAGATCAAGCAGGCGATGGAGGCTTCGCTGGAGGAAGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGCCTCCACAAGGAAGGCCGGATCGACATGGAAGAAGCGCTCAAGGCCGCCGACTCGCGCGAAGGCCTGTCGCTGAA
GTTCCGCCTGTCCGAAGGCGCCACGGGCGAACATGACCCGTACGCCGAGGTCTACGACGCGGTCACGCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

97.027

0.5
  pilU Acinetobacter baylyi ADP1

48.459

96.486

0.468
  pilU Vibrio cholerae strain A1552

47.74

95.676

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.251

96.757

0.438
  pilT Acinetobacter nosocomialis M2

40.988

92.973

0.381
  pilT Acinetobacter baumannii D1279779

40.988

92.973

0.381
  pilT Acinetobacter baumannii strain A118

40.988

92.973

0.381
  pilT Acinetobacter baylyi ADP1

40.698

92.973

0.378
  pilT Pseudomonas aeruginosa PAK

41.42

91.351

0.378
  pilT Pseudomonas stutzeri DSM 10701

40.116

92.973

0.373
  pilT Neisseria gonorrhoeae MS11

39.769

93.784

0.373
  pilT Neisseria meningitidis 8013

39.769

93.784

0.373
  pilT Legionella pneumophila strain Lp02

40.237

91.351

0.368
  pilT Legionella pneumophila strain ERS1305867

40.237

91.351

0.368