Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MN210_RS10245 Genome accession   NZ_CP093310
Coordinates   2442517..2443596 (+) Length   359 a.a.
NCBI ID   WP_338412164.1    Uniprot ID   A0AAU6PUS6
Organism   Psychrobacter raelei strain PraFG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2437517..2448596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN210_RS10220 (MN210_12955) - 2437865..2438338 (+) 474 WP_241878479.1 hypothetical protein -
  MN210_RS10225 (MN210_12960) - 2438508..2439023 (+) 516 WP_011961097.1 cell division protein ZapA -
  MN210_RS10230 (MN210_12970) - 2439030..2439479 (-) 450 WP_338412162.1 hypothetical protein -
  MN210_RS10235 (MN210_12975) - 2439878..2441536 (+) 1659 WP_241878481.1 NAD+ synthase -
  MN210_RS10240 (MN210_12980) - 2441643..2442359 (-) 717 WP_338412163.1 YggS family pyridoxal phosphate-dependent enzyme -
  MN210_RS10245 (MN210_12985) pilT 2442517..2443596 (+) 1080 WP_338412164.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MN210_RS10250 (MN210_12990) - 2443737..2445038 (+) 1302 WP_338412165.1 IS4 family transposase -
  MN210_RS10255 (MN210_12995) fur 2445133..2445564 (-) 432 WP_110816909.1 ferric iron uptake transcriptional regulator -
  MN210_RS10260 (MN210_13000) bamE 2445849..2446223 (+) 375 WP_011961103.1 outer membrane protein assembly factor BamE -
  MN210_RS10265 (MN210_13010) - 2446425..2446763 (-) 339 WP_011961104.1 RnfH family protein -
  MN210_RS10270 (MN210_13020) - 2446760..2447869 (-) 1110 WP_338412166.1 hypothetical protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39411.34 Da        Isoelectric Point: 7.0306

>NTDB_id=663451 MN210_RS10245 WP_338412164.1 2442517..2443596(+) (pilT) [Psychrobacter raelei strain PraFG1]
MIQGIDEVTHAHALDSLTALLRHTQSLKASDLHLSAGMPAMLRIDGEITSTSLPALTHTQIQQYIASTLSPAQMSEFEQH
MTLDYALDVADVGRFRVNIFQQHRGIAAVFRAIPDALLSLQQLDLSSDNKATLQQITQLNHGLVLVTGPTGCGKSTTVAA
LVDHINQTRAAHILTIEDPIEFIYHAKKSLINQREVARHTADYTKALRSALREDPDVIVIGELRDLASIRLALTAAETGH
LVLATLHTRSAIKSIDRIVDVFAADEKDMIRFLLSESLQAVISQILIKRPEGGRIAAHEIMVATPAICNMIRQNKLAQMY
SAIQTGHSQGMQTLDQALTDLIQRKLISPHSAQHHAKLC

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=663451 MN210_RS10245 WP_338412164.1 2442517..2443596(+) (pilT) [Psychrobacter raelei strain PraFG1]
ATGATTCAAGGGATAGATGAGGTGACACACGCTCACGCTTTAGATTCGCTCACTGCGCTACTACGCCATACTCAAAGCTT
AAAGGCTTCAGATCTGCACCTGTCAGCAGGAATGCCCGCCATGCTGCGAATCGATGGGGAGATAACCTCGACCAGTCTAC
CCGCCTTAACGCATACCCAAATTCAGCAGTATATTGCCAGCACCTTAAGCCCTGCGCAAATGAGTGAATTTGAACAGCAC
ATGACATTGGACTATGCGTTGGATGTGGCCGATGTAGGGCGATTTCGGGTCAATATATTTCAGCAGCACCGAGGTATTGC
AGCTGTATTTCGTGCCATCCCCGATGCCCTATTGTCTTTACAGCAGCTCGACTTATCCAGTGATAATAAGGCCACCTTGC
AACAAATAACCCAGCTCAATCATGGGCTGGTATTGGTCACCGGCCCCACCGGCTGCGGAAAATCAACCACAGTGGCCGCT
TTGGTTGATCATATTAACCAAACACGCGCCGCCCACATTCTGACCATCGAAGACCCCATTGAATTTATTTATCACGCCAA
AAAAAGCTTAATCAATCAGCGGGAAGTGGCGCGTCATACCGCAGATTATACGAAGGCCCTACGCTCAGCTTTGCGTGAAG
ATCCCGACGTTATTGTGATTGGTGAGCTGCGTGATTTGGCCAGTATACGTTTGGCGCTTACTGCAGCTGAGACCGGGCAT
TTGGTACTGGCAACGCTACATACCCGCTCTGCGATTAAAAGCATCGACAGAATTGTGGATGTATTTGCGGCCGATGAAAA
GGACATGATCCGATTTTTGTTGTCCGAATCGCTACAAGCTGTGATCTCGCAAATATTAATTAAGCGCCCTGAGGGCGGAC
GAATAGCCGCTCACGAGATTATGGTGGCCACCCCTGCCATCTGTAACATGATTCGTCAAAACAAACTGGCACAAATGTAT
TCCGCCATTCAAACTGGTCACAGCCAAGGCATGCAGACTCTAGACCAAGCGCTCACCGATCTAATCCAGCGCAAACTTAT
TAGCCCACATAGCGCTCAGCATCATGCCAAATTGTGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(26-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

56.891

94.986

0.54
  pilT Acinetobacter nosocomialis M2

56.891

94.986

0.54
  pilT Acinetobacter baumannii strain A118

56.891

94.986

0.54
  pilT Pseudomonas stutzeri DSM 10701

56.598

94.986

0.538
  pilT Pseudomonas aeruginosa PAK

56.14

95.265

0.535
  pilT Acinetobacter baylyi ADP1

55.718

94.986

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.971

95.265

0.524
  pilT Vibrio cholerae strain A1552

54.971

95.265

0.524
  pilT Legionella pneumophila strain ERS1305867

54.678

95.265

0.521
  pilT Legionella pneumophila strain Lp02

54.678

95.265

0.521
  pilT Neisseria meningitidis 8013

52.339

95.265

0.499
  pilT Neisseria gonorrhoeae MS11

52.047

95.265

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.84

97.214

0.426
  pilU Acinetobacter baylyi ADP1

39.826

95.822

0.382