Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MMZ72_RS23985 Genome accession   NZ_CP093021
Coordinates   5181106..5181555 (-) Length   149 a.a.
NCBI ID   WP_241469221.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5176106..5186555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ72_RS23965 (MMZ72_23965) ampD 5176798..5177364 (-) 567 WP_024914671.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MMZ72_RS23970 (MMZ72_23970) - 5177515..5179770 (-) 2256 WP_221921626.1 DUF1631 domain-containing protein -
  MMZ72_RS23975 (MMZ72_23975) nadC 5180056..5180904 (+) 849 WP_241469220.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MMZ72_RS23985 (MMZ72_23985) pilA 5181106..5181555 (-) 450 WP_241469221.1 type 4a pilus biogenesis protein PilA Machinery gene
  MMZ72_RS23990 (MMZ72_23990) pilB 5181788..5183488 (+) 1701 WP_024914668.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MMZ72_RS23995 (MMZ72_23995) pilC 5183492..5184712 (+) 1221 WP_024914667.1 type 4a pilus biogenesis protein PilC Machinery gene
  MMZ72_RS24000 (MMZ72_24000) pilD 5184716..5185588 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MMZ72_RS24005 (MMZ72_24005) coaE 5185585..5186196 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  MMZ72_RS24010 (MMZ72_24010) yacG 5186193..5186393 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15541.80 Da        Isoelectric Point: 6.2905

>NTDB_id=661503 MMZ72_RS23985 WP_241469221.1 5181106..5181555(-) (pilA) [Pseudomonas aeruginosa strain H09]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYV
GVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWTCKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=661503 MMZ72_RS23985 WP_241469221.1 5181106..5181555(-) (pilA) [Pseudomonas aeruginosa strain H09]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTACCAGAACTATGTAGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC
GGCGTCGAGCCGGATGCCAACAAGTTGGGTGTAATTGCTGTAGCAATCGAAGATAGCGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACCTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAACCGTACTGCGGATGGGGTCTGGACTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

67.785

100

0.678
  pilA Acinetobacter baumannii strain A118

48.667

100

0.49
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

48.993

100

0.49
  pilA Vibrio cholerae strain A1552

48.993

100

0.49
  pilA Vibrio cholerae C6706

48.993

100

0.49
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.638

100

0.456
  pilA Vibrio parahaemolyticus RIMD 2210633

44.898

98.658

0.443
  comP Acinetobacter baylyi ADP1

41.83

100

0.43
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.056

95.973

0.423
  pilA Acinetobacter nosocomialis M2

44.604

93.289

0.416
  pilA2 Legionella pneumophila str. Paris

39.456

98.658

0.389
  pilA2 Legionella pneumophila strain ERS1305867

38.776

98.658

0.383