Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MMZ72_RS24000 Genome accession   NZ_CP093021
Coordinates   5184716..5185588 (+) Length   290 a.a.
NCBI ID   WP_003125204.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5179716..5190588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ72_RS23975 (MMZ72_23975) nadC 5180056..5180904 (+) 849 WP_241469220.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MMZ72_RS23985 (MMZ72_23985) pilA 5181106..5181555 (-) 450 WP_241469221.1 type 4a pilus biogenesis protein PilA Machinery gene
  MMZ72_RS23990 (MMZ72_23990) pilB 5181788..5183488 (+) 1701 WP_024914668.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MMZ72_RS23995 (MMZ72_23995) pilC 5183492..5184712 (+) 1221 WP_024914667.1 type 4a pilus biogenesis protein PilC Machinery gene
  MMZ72_RS24000 (MMZ72_24000) pilD 5184716..5185588 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MMZ72_RS24005 (MMZ72_24005) coaE 5185585..5186196 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  MMZ72_RS24010 (MMZ72_24010) yacG 5186193..5186393 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  MMZ72_RS24015 (MMZ72_24015) - 5186431..5186640 (-) 210 WP_003094660.1 hypothetical protein -
  MMZ72_RS24020 (MMZ72_24020) - 5186746..5187435 (-) 690 WP_058141274.1 energy-coupling factor ABC transporter permease -
  MMZ72_RS24025 (MMZ72_24025) - 5187432..5187902 (-) 471 WP_024914665.1 hypothetical protein -
  MMZ72_RS24030 (MMZ72_24030) - 5187899..5188324 (-) 426 WP_024914664.1 GNAT family N-acetyltransferase -
  MMZ72_RS24035 (MMZ72_24035) - 5188457..5189086 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  MMZ72_RS24040 (MMZ72_24040) - 5189083..5189532 (+) 450 WP_024914662.1 MOSC domain-containing protein -
  MMZ72_RS24045 (MMZ72_24045) - 5189558..5189728 (+) 171 WP_058141275.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31839.89 Da        Isoelectric Point: 7.7068

>NTDB_id=661506 MMZ72_RS24000 WP_003125204.1 5184716..5185588(+) (pilD) [Pseudomonas aeruginosa strain H09]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=661506 MMZ72_RS24000 WP_003125204.1 5184716..5185588(+) (pilD) [Pseudomonas aeruginosa strain H09]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.714

96.552

0.538
  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472