Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MMZ72_RS23995 Genome accession   NZ_CP093021
Coordinates   5183492..5184712 (+) Length   406 a.a.
NCBI ID   WP_024914667.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5178492..5189712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ72_RS23975 (MMZ72_23975) nadC 5180056..5180904 (+) 849 WP_241469220.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MMZ72_RS23985 (MMZ72_23985) pilA 5181106..5181555 (-) 450 WP_241469221.1 type 4a pilus biogenesis protein PilA Machinery gene
  MMZ72_RS23990 (MMZ72_23990) pilB 5181788..5183488 (+) 1701 WP_024914668.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MMZ72_RS23995 (MMZ72_23995) pilC 5183492..5184712 (+) 1221 WP_024914667.1 type 4a pilus biogenesis protein PilC Machinery gene
  MMZ72_RS24000 (MMZ72_24000) pilD 5184716..5185588 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MMZ72_RS24005 (MMZ72_24005) coaE 5185585..5186196 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  MMZ72_RS24010 (MMZ72_24010) yacG 5186193..5186393 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  MMZ72_RS24015 (MMZ72_24015) - 5186431..5186640 (-) 210 WP_003094660.1 hypothetical protein -
  MMZ72_RS24020 (MMZ72_24020) - 5186746..5187435 (-) 690 WP_058141274.1 energy-coupling factor ABC transporter permease -
  MMZ72_RS24025 (MMZ72_24025) - 5187432..5187902 (-) 471 WP_024914665.1 hypothetical protein -
  MMZ72_RS24030 (MMZ72_24030) - 5187899..5188324 (-) 426 WP_024914664.1 GNAT family N-acetyltransferase -
  MMZ72_RS24035 (MMZ72_24035) - 5188457..5189086 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  MMZ72_RS24040 (MMZ72_24040) - 5189083..5189532 (+) 450 WP_024914662.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44346.12 Da        Isoelectric Point: 9.8784

>NTDB_id=661505 MMZ72_RS23995 WP_024914667.1 5183492..5184712(+) (pilC) [Pseudomonas aeruginosa strain H09]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGVIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSTVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=661505 MMZ72_RS23995 WP_024914667.1 5183492..5184712(+) (pilC) [Pseudomonas aeruginosa strain H09]
ATGGCGGACAAAGCGTTAAAAACCAGCGTATTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCAATGGACATCGCCCTGTTCACTCGGCAGATGGCGACCATGATGGGT
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGCTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCGCAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCCTTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGCGTGGGGGTGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAGAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCACGGTAGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGTAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGACGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baumannii D1279779

60.644

99.507

0.603
  pilC Acinetobacter baylyi ADP1

60.345

100

0.603
  pilC Legionella pneumophila strain ERS1305867

54.04

97.537

0.527
  pilG Neisseria meningitidis 44/76-A

45.906

99.261

0.456
  pilG Neisseria gonorrhoeae MS11

45.658

99.261

0.453
  pilC Vibrio cholerae strain A1552

42.365

100

0.424
  pilC Vibrio campbellii strain DS40M4

41.353

98.276

0.406
  pilC Thermus thermophilus HB27

36.658

98.768

0.362