Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKK04_RS24800 Genome accession   NZ_CP092827
Coordinates   5394372..5395370 (-) Length   332 a.a.
NCBI ID   WP_241106064.1    Uniprot ID   -
Organism   Pseudomonas sp. LS.1a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5389372..5400370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKK04_RS24780 (MKK04_24780) argS 5390439..5392175 (+) 1737 WP_207828261.1 arginine--tRNA ligase -
  MKK04_RS24785 (MKK04_24785) - 5392177..5392875 (+) 699 WP_063912064.1 SPOR domain-containing protein -
  MKK04_RS24790 (MKK04_24790) - 5393020..5393439 (-) 420 WP_063912065.1 TM2 domain-containing protein -
  MKK04_RS24795 (MKK04_24795) - 5393606..5394244 (+) 639 WP_207828256.1 C40 family peptidase -
  MKK04_RS24800 (MKK04_24800) pilT 5394372..5395370 (-) 999 WP_241106064.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKK04_RS24805 (MKK04_24805) - 5395436..5396122 (+) 687 WP_207828252.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKK04_RS24810 (MKK04_24810) proC 5396161..5396979 (+) 819 WP_207828250.1 pyrroline-5-carboxylate reductase -
  MKK04_RS24815 (MKK04_24815) - 5396990..5397580 (+) 591 WP_063912070.1 YggT family protein -
  MKK04_RS24820 (MKK04_24820) - 5397680..5398819 (+) 1140 WP_207828248.1 homoserine O-acetyltransferase -
  MKK04_RS24825 (MKK04_24825) metW 5398827..5399447 (+) 621 WP_003249106.1 methionine biosynthesis protein MetW -
  MKK04_RS24830 (MKK04_24830) - 5399470..5399904 (+) 435 WP_013974622.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35893.64 Da        Isoelectric Point: 6.6066

>NTDB_id=660535 MKK04_RS24800 WP_241106064.1 5394372..5395370(-) (pilT) [Pseudomonas sp. LS.1a]
MDVTDLLARAVDAGACDLHLAAGQIPMLRLDGELQRMALPALCSAALIEGLAPMLGEDQRRQWAQGDELDLALELPALGR
FRLNLFRQLHGPAATFRLIPGRIATLDELDLRDVFQAVAQCSDGLILVGGPTGSGKSSTLATLLDQLNRDRALHIITLED
PVEVIHSSQRSLVNQREIGRHCGGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVNSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRAGGGRVAAREVLVVTPAVRNLVREGRMAQLCSVMQTGGADGMRTMEGAM
RGLKERGLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=660535 MKK04_RS24800 WP_241106064.1 5394372..5395370(-) (pilT) [Pseudomonas sp. LS.1a]
ATGGATGTGACCGACCTGTTGGCCCGGGCTGTAGATGCGGGGGCTTGCGACCTGCACCTGGCAGCGGGGCAGATACCGAT
GTTGCGCCTGGATGGCGAGCTGCAGCGCATGGCCCTGCCGGCCTTGTGCTCGGCCGCCTTGATCGAGGGGCTGGCACCCA
TGTTGGGTGAAGACCAGCGCCGGCAATGGGCCCAGGGTGATGAGCTGGACCTGGCGCTGGAACTGCCTGCGCTCGGGCGC
TTTCGGCTGAACCTGTTTCGCCAGTTGCATGGCCCTGCCGCCACCTTTCGCCTGATCCCGGGGCGCATCGCCACGCTCGA
TGAACTCGACCTGAGGGATGTGTTTCAAGCTGTTGCGCAGTGCAGTGATGGCTTGATCCTGGTGGGTGGGCCGACCGGCA
GCGGCAAGTCCAGCACCCTGGCGACGCTACTGGACCAGCTGAACCGTGATCGGGCACTGCATATCATTACCCTCGAAGAC
CCTGTCGAAGTTATCCACAGCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGATCGGTCGCCACTGCGGTGGGTTCGCCCA
AGGGCTACGCAGTGCCCTGCGCCAGGACCCGGATGTGATCATGATCGGTGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCTTGCGCGCGGCCGAGACCGGGCACCTGGTGCTGGCGACCGTGCATACGCGTTCGGCGGTTAACAGCATTGACCGGTTG
GTGGAGGTGTTTGCTGCCGAGGAAAAGCCGCTGGTGCGGGCGATGCTGGCCGAGTCATTGCGCCTGGTGGTGGCGCAGGT
ACTGGTCAGGCGTGCGGGCGGTGGGCGGGTTGCGGCGCGGGAAGTGCTGGTGGTGACGCCAGCGGTGCGCAACCTGGTTC
GGGAGGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGACGGGCGGTGCGGACGGGATGCGGACGATGGAGGGGGCGATG
CGAGGGTTGAAGGAGAGGGGGCTGATCAGCGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.818

99.398

0.515
  pilT Acinetobacter nosocomialis M2

51.212

99.398

0.509
  pilT Acinetobacter baumannii D1279779

51.212

99.398

0.509
  pilT Acinetobacter baumannii strain A118

51.212

99.398

0.509
  pilT Pseudomonas stutzeri DSM 10701

50.909

99.398

0.506
  pilT Acinetobacter baylyi ADP1

50.606

99.398

0.503
  pilT Vibrio cholerae strain A1552

49.544

99.096

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

99.096

0.491
  pilT Legionella pneumophila strain Lp02

49.091

99.398

0.488
  pilT Legionella pneumophila strain ERS1305867

49.091

99.398

0.488
  pilT Neisseria meningitidis 8013

48.338

99.699

0.482
  pilT Neisseria gonorrhoeae MS11

48.036

99.699

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.619

99.699

0.455
  pilU Pseudomonas stutzeri DSM 10701

36.254

99.699

0.361