Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L2Y54_RS05575 Genome accession   NZ_CP091244
Coordinates   1093592..1094749 (+) Length   385 a.a.
NCBI ID   WP_236500805.1    Uniprot ID   -
Organism   Thiothrix winogradskyi strain CT3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1088592..1099749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y54_RS05555 (L2Y54_05555) - 1089539..1091059 (-) 1521 WP_236500801.1 aldehyde dehydrogenase family protein -
  L2Y54_RS05560 (L2Y54_05560) - 1091301..1091792 (+) 492 WP_236500802.1 DUF4124 domain-containing protein -
  L2Y54_RS05565 (L2Y54_05565) - 1091796..1092482 (-) 687 WP_236500804.1 YggS family pyridoxal phosphate-dependent enzyme -
  L2Y54_RS05570 (L2Y54_05570) pilT 1092544..1093578 (+) 1035 WP_202715528.1 type IV pilus twitching motility protein PilT Machinery gene
  L2Y54_RS05575 (L2Y54_05575) pilU 1093592..1094749 (+) 1158 WP_236500805.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Y54_RS05580 (L2Y54_05580) - 1094746..1095876 (+) 1131 WP_236500806.1 PilT/PilU family type 4a pilus ATPase -
  L2Y54_RS05585 (L2Y54_05585) bioD 1095873..1096532 (-) 660 WP_236500807.1 dethiobiotin synthase -
  L2Y54_RS05590 (L2Y54_05590) - 1096884..1097336 (+) 453 WP_236500808.1 DUF1566 domain-containing protein -
  L2Y54_RS05595 (L2Y54_05595) - 1097451..1099028 (-) 1578 WP_236500809.1 TolC family outer membrane protein -
  L2Y54_RS05600 (L2Y54_05600) - 1099111..1099425 (-) 315 WP_236500810.1 rhodanese-like domain-containing protein -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 43024.64 Da        Isoelectric Point: 7.5372

>NTDB_id=648789 L2Y54_RS05575 WP_236500805.1 1093592..1094749(+) (pilU) [Thiothrix winogradskyi strain CT3]
MDRDAAVSYVHKLLATMLEKKASDLYVTAEAAPSAKISSEMLPLTNQPLNEQTARMLVRAIMNDRQLKQFEEDMECNFSI
SLPGKARFRVNAFTQRGCAGMVLRHIPNHIPGLKDLGLPPVLRDIAMTKRGLVIMVGATGSGKSTTLASMVDFRNTNSKG
HIVTIEDPIEFVHQHKGCLITQREVGVDTASYEIAMKNTLRQAPDVILLGEIRDRESMDYAIAYAETGHLCLSTLHANSS
NQAIDRIINFFPEERRNQLLMDLSLNLKAVVSQRLVRKAKGEGRLPAVEVVINTPLMADLILKGDVPGMKILTSKSREHG
MRTFDQALFDLIEARQITVHEALRHADSQNDLRLRLKLESRFAQDNDFFKGLDDLAIEPLENTRA

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=648789 L2Y54_RS05575 WP_236500805.1 1093592..1094749(+) (pilU) [Thiothrix winogradskyi strain CT3]
ATGGATCGCGATGCTGCTGTCAGCTACGTGCATAAGTTATTGGCGACTATGTTGGAAAAAAAGGCTTCCGACTTGTATGT
TACGGCGGAGGCGGCTCCTTCTGCCAAAATTTCCAGCGAAATGCTGCCATTAACCAATCAGCCACTCAATGAGCAAACGG
CGCGGATGTTGGTGCGGGCGATTATGAATGACCGCCAGCTCAAACAGTTCGAGGAGGACATGGAATGCAATTTTTCCATT
AGTCTGCCGGGCAAAGCGCGATTCCGGGTGAATGCGTTCACGCAACGCGGTTGTGCGGGGATGGTCTTGCGCCATATTCC
CAACCATATTCCGGGGTTGAAGGATTTGGGTTTGCCGCCGGTCTTGCGCGATATTGCGATGACCAAGCGCGGTTTGGTGA
TTATGGTGGGGGCGACCGGTTCAGGTAAATCGACCACACTGGCATCAATGGTCGATTTCCGCAATACCAATTCCAAAGGG
CATATTGTCACGATTGAAGACCCGATTGAGTTTGTGCATCAGCACAAAGGGTGCTTGATTACGCAGCGCGAAGTGGGTGT
CGATACCGCCAGCTATGAAATCGCGATGAAGAATACCTTGCGGCAAGCTCCTGATGTGATTTTGCTGGGGGAGATCCGTG
ACCGTGAGTCGATGGATTACGCGATTGCCTACGCCGAAACTGGGCATTTGTGCTTGTCGACCTTGCACGCCAACAGTTCC
AACCAAGCGATTGACCGTATTATCAACTTTTTCCCGGAAGAACGCCGCAATCAATTATTGATGGATTTGTCGCTTAATCT
TAAGGCGGTGGTCTCACAGCGGCTGGTGCGTAAAGCCAAAGGGGAAGGGCGTTTGCCTGCGGTGGAGGTGGTTATTAATA
CCCCATTAATGGCAGATTTAATCCTGAAAGGCGATGTGCCGGGCATGAAGATTCTGACCTCAAAATCGCGTGAACACGGA
ATGCGCACCTTCGACCAAGCGCTATTTGATCTGATTGAGGCACGGCAAATCACGGTGCATGAAGCTTTACGTCATGCGGA
TTCGCAGAATGACTTGCGCCTGCGTCTCAAACTGGAAAGCCGTTTCGCGCAAGATAATGACTTCTTCAAGGGGTTGGATG
ATTTGGCGATTGAACCACTGGAGAATACCCGCGCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.599

90.649

0.54
  pilU Acinetobacter baylyi ADP1

56.484

90.13

0.509
  pilU Vibrio cholerae strain A1552

51.149

90.39

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

87.532

0.395
  pilT Acinetobacter baumannii D1279779

43.529

88.312

0.384
  pilT Acinetobacter baumannii strain A118

43.529

88.312

0.384
  pilT Acinetobacter nosocomialis M2

43.235

88.312

0.382
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.261

90.649

0.374
  pilT Vibrio cholerae strain A1552

41.261

90.649

0.374
  pilT Legionella pneumophila strain Lp02

43.243

86.494

0.374
  pilT Legionella pneumophila strain ERS1305867

43.243

86.494

0.374
  pilT Acinetobacter baylyi ADP1

43.963

83.896

0.369
  pilT Pseudomonas stutzeri DSM 10701

43.034

83.896

0.361