Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L2Y54_RS05570 Genome accession   NZ_CP091244
Coordinates   1092544..1093578 (+) Length   344 a.a.
NCBI ID   WP_202715528.1    Uniprot ID   -
Organism   Thiothrix winogradskyi strain CT3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1087544..1098578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y54_RS05550 (L2Y54_05550) - 1088455..1089474 (-) 1020 WP_236500800.1 protein adenylyltransferase SelO family protein -
  L2Y54_RS05555 (L2Y54_05555) - 1089539..1091059 (-) 1521 WP_236500801.1 aldehyde dehydrogenase family protein -
  L2Y54_RS05560 (L2Y54_05560) - 1091301..1091792 (+) 492 WP_236500802.1 DUF4124 domain-containing protein -
  L2Y54_RS05565 (L2Y54_05565) - 1091796..1092482 (-) 687 WP_236500804.1 YggS family pyridoxal phosphate-dependent enzyme -
  L2Y54_RS05570 (L2Y54_05570) pilT 1092544..1093578 (+) 1035 WP_202715528.1 type IV pilus twitching motility protein PilT Machinery gene
  L2Y54_RS05575 (L2Y54_05575) pilU 1093592..1094749 (+) 1158 WP_236500805.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Y54_RS05580 (L2Y54_05580) - 1094746..1095876 (+) 1131 WP_236500806.1 PilT/PilU family type 4a pilus ATPase -
  L2Y54_RS05585 (L2Y54_05585) bioD 1095873..1096532 (-) 660 WP_236500807.1 dethiobiotin synthase -
  L2Y54_RS05590 (L2Y54_05590) - 1096884..1097336 (+) 453 WP_236500808.1 DUF1566 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38186.73 Da        Isoelectric Point: 8.1677

>NTDB_id=648788 L2Y54_RS05570 WP_202715528.1 1092544..1093578(+) (pilT) [Thiothrix winogradskyi strain CT3]
MDITQLLAFSVKNNASDLHLSGGMPPMIRVDGEMRKINLPVLDHKQVHALVYDIMNDHQRKNYEEHWECDFSFEIPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDQLGAPGIFRKISENPRGLVLVTGPTGSGKSTTLAAMVDYKNDTDYGHILTIED
PIEFVHESKKSLVNQREVHRDTKGFEAALRSALREDPDTILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPAAEKEMVRSMLSESLQAVISQTLLKKKGGGRVAAHEILIGNRAVRNLIRENKIAQMRSAMQTGQSDGMQTLDQSL
KNLVMKGLVDREAARRKADNPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=648788 L2Y54_RS05570 WP_202715528.1 1092544..1093578(+) (pilT) [Thiothrix winogradskyi strain CT3]
ATGGACATTACCCAACTATTGGCGTTCAGCGTCAAAAACAATGCATCCGACTTGCACTTGTCGGGCGGGATGCCTCCCAT
GATCCGTGTCGATGGCGAAATGCGCAAGATCAACTTGCCGGTGCTCGACCATAAACAAGTACACGCGCTGGTGTACGACA
TCATGAATGATCACCAACGTAAAAACTACGAAGAGCATTGGGAATGCGATTTTTCGTTTGAAATTCCCGGTGTGGCGCGG
TTTCGTGTGAATGCCTTTAACCAAAACCGTGGCGCAGGTGCGGTGTTTCGTACCATCCCCAGCAAAGTATTAACGCTGGA
TCAGTTGGGTGCGCCAGGTATTTTTCGGAAAATTTCGGAAAACCCACGCGGCTTGGTGTTGGTGACAGGGCCGACCGGTT
CGGGTAAATCCACCACCCTCGCGGCGATGGTCGATTACAAAAATGATACCGATTACGGGCATATTTTGACCATTGAAGAC
CCGATCGAATTCGTGCACGAAAGCAAAAAAAGTTTGGTCAACCAACGTGAAGTGCATCGCGATACCAAAGGCTTTGAAGC
CGCCCTCCGTTCCGCCTTGCGTGAAGACCCGGATACCATTCTGGTCGGGGAAATGCGTGACTTGGAAACGATTCGCTTGG
CACTAACCGCAGCAGAAACCGGGCATTTGGTATTCGGCACGTTGCATACGACATCCGCACCCAAAACCATTGACCGTATT
GTGGACGTGTTCCCCGCTGCCGAAAAAGAGATGGTGCGTTCCATGCTTTCCGAATCCTTGCAAGCGGTGATTTCGCAAAC
TTTGCTGAAGAAAAAAGGTGGGGGGCGCGTGGCAGCACACGAAATTCTGATTGGCAATCGGGCGGTGCGTAACCTGATTC
GTGAAAATAAGATTGCGCAAATGCGTTCTGCGATGCAAACCGGGCAAAGTGACGGGATGCAAACCCTTGATCAATCACTG
AAAAACTTGGTGATGAAAGGTTTGGTGGATCGTGAAGCGGCGCGGCGTAAAGCCGATAACCCGGATTCTATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.36

100

0.794
  pilT Pseudomonas aeruginosa PAK

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

77.907

100

0.779
  pilT Acinetobacter baumannii strain A118

77.907

100

0.779
  pilT Acinetobacter nosocomialis M2

77.907

100

0.779
  pilT Acinetobacter baylyi ADP1

76.453

100

0.765
  pilT Neisseria gonorrhoeae MS11

71.594

100

0.718
  pilT Neisseria meningitidis 8013

71.594

100

0.718
  pilT Legionella pneumophila strain Lp02

71.765

98.837

0.709
  pilT Legionella pneumophila strain ERS1305867

71.765

98.837

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.257

98.547

0.663
  pilT Vibrio cholerae strain A1552

67.257

98.547

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

40.87

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395