Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SMGD1_RS12210 Genome accession   NZ_AFRZ01000001
Coordinates   2474404..2475486 (-) Length   360 a.a.
NCBI ID   WP_008339800.1    Uniprot ID   B6BN90
Organism   Sulfurimonas gotlandica GD1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2469404..2480486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMGD1_RS12185 (SMGD1_2431) - 2470282..2471493 (+) 1212 WP_008339874.1 TolC family protein -
  SMGD1_RS12190 (SMGD1_2432) - 2471483..2472478 (+) 996 WP_008339778.1 SAM-dependent methyltransferase -
  SMGD1_RS14660 - 2472519..2472719 (+) 201 WP_139064136.1 hypothetical protein -
  SMGD1_RS12195 (SMGD1_2434) - 2472738..2473355 (-) 618 WP_008339888.1 YkgJ family cysteine cluster protein -
  SMGD1_RS12200 (SMGD1_2435) - 2473567..2473800 (-) 234 WP_241761486.1 hypothetical protein -
  SMGD1_RS12205 (SMGD1_2436) - 2473862..2474320 (-) 459 WP_008339842.1 nucleoside deaminase -
  SMGD1_RS12210 (SMGD1_2437) pilT 2474404..2475486 (-) 1083 WP_008339800.1 type IV pilus twitching motility protein PilT Machinery gene
  SMGD1_RS12215 (SMGD1_2438) gatC 2475503..2475793 (-) 291 WP_008339642.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  SMGD1_RS12220 (SMGD1_2439) - 2475921..2476517 (+) 597 WP_008339748.1 hypothetical protein -
  SMGD1_RS12225 (SMGD1_2440) - 2476522..2477520 (+) 999 WP_008339623.1 hypothetical protein -
  SMGD1_RS12230 (SMGD1_2441) - 2477495..2478325 (+) 831 WP_316680473.1 tyrosine-type recombinase/integrase -
  SMGD1_RS12235 (SMGD1_2442) - 2478418..2478894 (+) 477 WP_008339836.1 HD domain-containing protein -
  SMGD1_RS12240 (SMGD1_2443) - 2479103..2479609 (+) 507 WP_008339639.1 hypothetical protein -
  SMGD1_RS12245 (SMGD1_2444) - 2479609..2480319 (+) 711 WP_008339740.1 sulfite exporter TauE/SafE family protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39956.02 Da        Isoelectric Point: 6.1859

>NTDB_id=64663 SMGD1_RS12210 WP_008339800.1 2474404..2475486(-) (pilT) [Sulfurimonas gotlandica GD1]
MSEEVKKVVDIKKLLKSVLAYGSSDLHLVVGSEPQIRIDKELRPLNLAVLNAKDIEEMAYSLIEDKQKKVFEDANELDFS
FELKEVGRFRANYYRTIGGIACAFRMIPIDIPTLDEYGNPPIFKELVKKEKGLILVTGPTGSGKSTTLASMLHEINLTER
RHIITVEDPVEFVHKNIKSLFSQRDVGNDTASFAAALKFALRQDPDIILIGEMRDAETIGAALTAAETGHLVFGTLHTNS
APGTINRIIDVFDGQEQAQVRAQLASSLVAVISQTLIPRVGGGKVAALEILITNPAVQNQIREDKVHQIYSQMQLNQKET
NMTTQTQELIELLQKRTITKENAIKNSNRPEELIKMIGGL

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=64663 SMGD1_RS12210 WP_008339800.1 2474404..2475486(-) (pilT) [Sulfurimonas gotlandica GD1]
ATGAGTGAAGAAGTCAAAAAAGTAGTTGATATAAAAAAACTGCTCAAGAGTGTTTTAGCCTACGGTTCAAGTGATTTACA
CCTTGTTGTTGGTAGTGAACCACAGATTAGAATAGATAAAGAACTTCGTCCACTTAATTTGGCTGTTTTAAATGCCAAAG
ATATTGAAGAGATGGCTTACTCGCTCATAGAAGACAAGCAAAAGAAAGTATTTGAAGATGCTAATGAACTTGACTTTTCT
TTTGAGTTAAAAGAAGTTGGACGTTTTCGTGCCAACTACTACAGAACTATCGGTGGGATAGCCTGTGCTTTTCGTATGAT
ACCTATAGATATTCCAACTCTTGATGAGTATGGAAATCCTCCAATTTTTAAAGAACTTGTAAAAAAAGAAAAAGGTCTAA
TTCTAGTAACAGGTCCTACAGGTAGTGGTAAATCAACTACTCTCGCATCTATGCTTCATGAAATAAACCTGACAGAAAGA
AGACATATAATCACTGTCGAAGATCCAGTTGAGTTCGTTCATAAAAACATCAAGTCACTATTTTCTCAAAGAGATGTAGG
AAACGATACTGCTTCATTTGCTGCTGCATTAAAATTTGCACTAAGACAAGATCCAGATATTATACTAATCGGTGAAATGA
GAGATGCTGAGACTATTGGTGCAGCTTTAACAGCAGCTGAGACTGGTCACTTGGTTTTTGGAACTCTGCACACAAACTCT
GCACCTGGAACTATTAACCGTATCATTGATGTTTTTGATGGACAAGAGCAAGCACAAGTAAGAGCGCAACTGGCTTCTTC
ACTTGTTGCTGTAATATCTCAAACACTTATTCCTAGAGTAGGTGGTGGCAAAGTTGCAGCTCTGGAAATACTCATAACTA
ATCCAGCTGTTCAGAATCAGATACGTGAAGACAAAGTTCACCAGATTTACTCTCAAATGCAGCTAAATCAAAAAGAAACG
AACATGACCACTCAGACTCAAGAGCTGATAGAACTACTTCAAAAAAGAACTATCACAAAAGAGAACGCTATTAAAAATTC
TAATAGACCCGAAGAGCTTATAAAAATGATAGGAGGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B6BN90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.594

95.833

0.494
  pilT Acinetobacter baumannii D1279779

51.156

96.111

0.492
  pilT Acinetobacter nosocomialis M2

51.156

96.111

0.492
  pilT Acinetobacter baumannii strain A118

51.156

96.111

0.492
  pilT Legionella pneumophila strain Lp02

52.537

93.056

0.489
  pilT Legionella pneumophila strain ERS1305867

52.537

93.056

0.489
  pilT Acinetobacter baylyi ADP1

51.014

95.833

0.489
  pilT Pseudomonas stutzeri DSM 10701

50.435

95.833

0.483
  pilT Neisseria gonorrhoeae MS11

49.853

94.722

0.472
  pilT Neisseria meningitidis 8013

49.128

95.556

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.376

90.833

0.467
  pilT Vibrio cholerae strain A1552

51.376

90.833

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.278

96.944

0.458
  pilU Acinetobacter baylyi ADP1

38.905

96.389

0.375

Multiple sequence alignment