Detailed information NTG0643200

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OHM01_RS09930
• Genome accession: NZ_CP107274
• Coordinates: 1887269..1888495 (-)
• Length: 408 a.a.
• NCBI ID: WP_003697786.1
• Uniprot ID: B4RPS5
• Organism: Neisseria gonorrhoeae strain 1145734
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1882269..1893495

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OHM01_RS09920 (OHM01_09920)	-	1883786..1884070 (+)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
OHM01_RS09925 (OHM01_09925)	yccS	1885002..1887152 (-)	2151	WP_003697785.1	YccS family putative transporter	-
OHM01_RS09930 (OHM01_09930)	pilU	1887269..1888495 (-)	1227	WP_003697786.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OHM01_RS09935 (OHM01_09935)	pilT	1888659..1889702 (-)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
OHM01_RS09940 (OHM01_09940)	-	1889822..1890508 (+)	687	WP_003699139.1	YggS family pyridoxal phosphate-dependent enzyme	-
OHM01_RS09945 (OHM01_09945)	-	1890508..1890915 (+)	408	WP_003690202.1	hypothetical protein	-
OHM01_RS09950 (OHM01_09950)	proC	1890964..1891755 (+)	792	WP_263936396.1	pyrroline-5-carboxylate reductase	-
OHM01_RS09955 (OHM01_09955)	dksA	1891903..1892319 (+)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45668.30 Da
• Isoelectric Point: 7.2909

>NTDB_id=643200 OHM01_RS09930 WP_003697786.1 1887269..1888495(-) (pilU) [Neisseria gonorrhoeae strain 1145734]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=643200 OHM01_RS09930 WP_003697786.1 1887269..1888495(-) (pilU) [Neisseria gonorrhoeae strain 1145734]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: B4RPS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387