Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LZ683_RS25255 Genome accession   NZ_CP090445
Coordinates   5406790..5407926 (+) Length   378 a.a.
NCBI ID   WP_167003749.1    Uniprot ID   -
Organism   Comamonas testosteroni strain X13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5401790..5412926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ683_RS25235 (LZ683_25200) - 5402654..5403424 (+) 771 WP_182284826.1 TetR/AcrR family transcriptional regulator -
  LZ683_RS25240 (LZ683_25205) - 5403472..5404719 (+) 1248 WP_182284827.1 branched-chain amino acid ABC transporter substrate-binding protein -
  LZ683_RS25245 (LZ683_25210) - 5404852..5405583 (-) 732 WP_182284828.1 YggS family pyridoxal phosphate-dependent enzyme -
  LZ683_RS25250 (LZ683_25215) pilT 5405621..5406664 (+) 1044 WP_003050584.1 type IV pilus twitching motility protein PilT Machinery gene
  LZ683_RS25255 (LZ683_25220) pilU 5406790..5407926 (+) 1137 WP_167003749.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LZ683_RS25260 (LZ683_25225) - 5408084..5408995 (+) 912 WP_182284829.1 NAD(P)-dependent oxidoreductase -
  LZ683_RS25265 (LZ683_25230) - 5409055..5409708 (-) 654 WP_003068458.1 BON domain-containing protein -
  LZ683_RS25270 (LZ683_25235) - 5409705..5410313 (-) 609 WP_003068456.1 SIS domain-containing protein -
  LZ683_RS25275 (LZ683_25240) - 5410403..5410804 (-) 402 WP_182284830.1 YraN family protein -
  LZ683_RS25280 (LZ683_25245) rsmI 5410804..5411727 (+) 924 WP_182284831.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42049.55 Da        Isoelectric Point: 6.7687

>NTDB_id=642499 LZ683_RS25255 WP_167003749.1 5406790..5407926(+) (pilU) [Comamonas testosteroni strain X13]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGKVTKVSPQPLSQLHTLALARSIMNDRQVADFEHTKECNFAI
SPAGVGRFRVNAFMQQGKVGMVLRTIPTTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTESWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDVSLNLRAIISQRLIPKQDSKGRVAAIEVMLNSPLIADLIFKGEVVEIKEIMKKSRNIG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLNSQRARTLDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=642499 LZ683_RS25255 WP_167003749.1 5406790..5407926(+) (pilU) [Comamonas testosteroni strain X13]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACTGCAGAGTTTCCGCCAGCCATCAAGATTGACGGCAAGGTCACCAAGGTCTCGCCCCAGCCGCTGAGCCAGCTGCATA
CGCTGGCCCTGGCGCGTTCCATCATGAACGACAGGCAGGTGGCGGACTTCGAGCACACCAAGGAGTGCAACTTCGCCATC
TCGCCGGCAGGCGTGGGGCGCTTCCGTGTCAATGCCTTCATGCAGCAGGGCAAGGTCGGCATGGTGCTGCGTACCATTCC
CACCACCCTGCCCACGATTGACGGCCTGGGCGTCCCACAGGTGCTCAAGGAAGTGACCATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCGACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCGGTGGAATTCGTGCATCCGCACAAGAACTGCGTGGTCACGCAACGCGAGGTGGGGCT
GGATACGGAAAGCTGGGAGGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGATGTGATTCTGATGGGCGAAATCCGTG
ACCGCGAAACCATGGAGCACGCGATTGCGTTCTCTGAAACCGGCCACCTTTGCCTGGCCACGCTGCACGCCAACAGTGCC
AACCAGGCGCTGGATCGCATCGTCAACTTCTTCCCCGAAGAGCGCCGTCCTCAGCTGCTGATGGATGTGTCGCTGAATCT
GCGGGCCATCATTTCCCAGCGCCTGATCCCCAAGCAGGACAGCAAGGGCCGTGTAGCCGCCATCGAGGTGATGCTCAACT
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGAGGTCGTCGAGATCAAGGAGATCATGAAGAAAAGCCGCAATATCGGC
ATGCAGACCTTTGACCAGGCGCTGTTCGACCTGTTCGAATCCAATCTCATCACCTACGAAGATGCGCTGCGCAATGCAGA
CTCGGTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCCAGCGTGCCCGGACTCTGGACCTGGCGGCCGGCACCGAGC
ATCTGACTATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.202

96.825

0.593
  pilU Acinetobacter baylyi ADP1

55.989

94.974

0.532
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362
  pilT Acinetobacter baylyi ADP1

41.768

86.772

0.362