Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LXG94_RS25235 Genome accession   NZ_CP090179
Coordinates   3197754..3198860 (+) Length   368 a.a.
NCBI ID   WP_017817653.1    Uniprot ID   -
Organism   Vibrio harveyi strain XH2145     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3192754..3203860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXG94_RS25200 (LXG94_25200) - 3192893..3193495 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  LXG94_RS25205 (LXG94_25205) - 3193601..3194032 (-) 432 WP_005425804.1 DUF4426 domain-containing protein -
  LXG94_RS25210 (LXG94_25210) yggU 3194092..3194382 (-) 291 WP_005425805.1 DUF167 family protein YggU -
  LXG94_RS25215 (LXG94_25215) - 3194382..3194939 (-) 558 WP_005425806.1 YggT family protein -
  LXG94_RS25220 (LXG94_25220) proC 3194993..3195811 (-) 819 WP_038899445.1 pyrroline-5-carboxylate reductase -
  LXG94_RS25225 (LXG94_25225) - 3195943..3196653 (-) 711 WP_009697582.1 YggS family pyridoxal phosphate-dependent enzyme -
  LXG94_RS25230 (LXG94_25230) pilT 3196682..3197722 (+) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  LXG94_RS25235 (LXG94_25235) pilU 3197754..3198860 (+) 1107 WP_017817653.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LXG94_RS25240 (LXG94_25240) ruvX 3198920..3199345 (-) 426 WP_005449738.1 Holliday junction resolvase RuvX -
  LXG94_RS25245 (LXG94_25245) - 3199395..3199958 (-) 564 WP_005449740.1 YqgE/AlgH family protein -
  LXG94_RS25250 (LXG94_25250) gshB 3200088..3201038 (-) 951 WP_005449741.1 glutathione synthase -
  LXG94_RS25255 (LXG94_25255) rsmE 3201053..3201784 (-) 732 WP_005449742.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LXG94_RS25260 (LXG94_25260) endA 3201931..3202626 (-) 696 WP_005449743.1 deoxyribonuclease I -
  LXG94_RS25265 (LXG94_25265) - 3202778..3203275 (-) 498 WP_009697583.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41277.40 Da        Isoelectric Point: 6.7730

>NTDB_id=640988 LXG94_RS25235 WP_017817653.1 3197754..3198860(+) (pilU) [Vibrio harveyi strain XH2145]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLSENDVALLLDSAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKATMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=640988 LXG94_RS25235 WP_017817653.1 3197754..3198860(+) (pilU) [Vibrio harveyi strain XH2145]
ATGGATCTGAATAAATTTCTCGAAGGCATGCTGGCGTTAAAAGCGTCGGATCTATACATCACTGTCGGTGCCCCGATCTT
GTTTCGTGTGGATGGCGAATTGCGTCCGCAAGGCGATAAGCTGAGCGAAAATGACGTAGCCTTGTTGCTTGATAGTGCAA
TGGACCCGGAGCGACGACAAGAGTTTCGCAAAAGCCGTGAGTCGAACTTTGCCATTGTCCGTGATTGCGGTCGCTTCCGT
GTCAGTGCCTTCTTCCAACGTGAATTACCGGGAGCCGTCATTCGTCGTATTGAAACCAATATTCCAACGTTTGAGCAGCT
TAAGTTGCCTTTGGTGCTGCAAGATCTTGCAATTGCCAAGCGTGGTTTAGTGCTGGTGGTTGGTGCGACAGGCTCTGGTA
AGTCGACCACCATGGCTGCGATGACGGGTTATCGCAACAACAACAAGACTGGTCATATTCTTACGGTAGAAGACCCAATT
GAATTTGTGCATGAGCATAAACGTTGTATTGTGACTCAGCGTGAAGTGGGCTTGGATACTGACAGCTATGAAGTGGCGTT
GAAGAATTCGTTGCGTCAGGCACCAGACATGATTTTGATTGGTGAGATCCGAAGCCGAGAAACGATGGAATACGCAATGA
CCTTTGCTGAAACTGGTCACTTGTGCATGGCTACATTGCACGCCAACAACGCAAACCAAGCACTTGAACGTATTTTGCAC
TTGGTGCCGAAAGACCAGAAAGATCAGTTCTTGTTTGATCTGTCGATGAACTTAAAAGGCGTAGTAGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAATAGCCCGCGTGTATCCGATCTGATCCGTC
GAGGCGATCTTCATGAGTTGAAAGCAACCATGGCTCGATCCAATGAATTTGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTAGTGATGCAAGGTAAGATCAGTGAAGAAGATGCATTGCACAGTGCGGACTCTGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

59.143

95.109

0.563
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.57

93.478

0.389
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.643

92.935

0.378
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37