Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LVB77_RS18950 Genome accession   NZ_CP089924
Coordinates   4220713..4221825 (+) Length   370 a.a.
NCBI ID   WP_056178868.1    Uniprot ID   A0A0Q8CXP1
Organism   Lysobacter sp. 5GHs7-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4215713..4226825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVB77_RS18925 (LVB77_18925) - 4216169..4217113 (-) 945 WP_232907689.1 2OG-Fe(II) oxygenase -
  LVB77_RS18930 (LVB77_18930) - 4217110..4218480 (-) 1371 WP_232907690.1 pyridoxal-phosphate dependent enzyme -
  LVB77_RS18935 (LVB77_18935) - 4218786..4219004 (-) 219 WP_232907692.1 YdcH family protein -
  LVB77_RS18940 (LVB77_18940) - 4219336..4220226 (-) 891 WP_232907693.1 hypothetical protein -
  LVB77_RS18945 (LVB77_18945) - 4220223..4220582 (-) 360 WP_232907695.1 DUF4398 domain-containing protein -
  LVB77_RS18950 (LVB77_18950) pilU 4220713..4221825 (+) 1113 WP_056178868.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVB77_RS18955 (LVB77_18955) - 4221853..4222116 (+) 264 WP_232907697.1 hypothetical protein -
  LVB77_RS18960 (LVB77_18960) - 4222137..4222520 (-) 384 WP_232907698.1 hypothetical protein -
  LVB77_RS18965 (LVB77_18965) maiA 4222550..4223212 (-) 663 WP_232907699.1 maleylacetoacetate isomerase -
  LVB77_RS18970 (LVB77_18970) - 4223308..4224297 (-) 990 WP_232907701.1 fumarylacetoacetate hydrolase family protein -
  LVB77_RS18980 (LVB77_18980) rpsI 4224853..4225245 (-) 393 WP_055908877.1 30S ribosomal protein S9 -
  LVB77_RS18985 (LVB77_18985) rplM 4225261..4225689 (-) 429 WP_056178849.1 50S ribosomal protein L13 -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41112.34 Da        Isoelectric Point: 6.0548

>NTDB_id=639119 LVB77_RS18950 WP_056178868.1 4220713..4221825(+) (pilU) [Lysobacter sp. 5GHs7-4]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSIIPSIEELQLPQVLKEIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSNTSGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFNESAHKNVLMNLALNLKAVVSQRLVVGTDGRRLPAAEILINTPHVRDLMRRGQVHEIKQAMEESLEEGMESFDQCL
FRLYKEGRIEMEEALKAADSRDGLALKFRLSEGGAEHDPYADMYDANAAH

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=639119 LVB77_RS18950 WP_056178868.1 4220713..4221825(+) (pilU) [Lysobacter sp. 5GHs7-4]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAAAACGCGTCGGACATGTTCCTGACCACCGGGGCGCCGGTTTA
CATCAAGGTCGAGGGCAGACTGTACCCGCTCGGCAACACCGGGTTGCCCCCGGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAGCTGAACATGGCCCTGGCGCTGCCCGATGCCGGCCGC
TTCCGCATCAACGTATTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCCATCCGCAGCATCATTCCCAGCATCGA
GGAACTGCAGCTGCCGCAGGTGCTCAAGGAGATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTGGGCAGCACCGGCT
CGGGCAAGTCGACGACGCTGGCGTCGATGATCGATCATCGCAACAGCAATACCTCCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTATCTGCACAAGCATAAGAAATCGATCGTGAACCAGCGCGAGGTCGGCCTGGACACGCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTCGACGCGACCACGATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACGCTGGAACGCATC
CTCAACTTCTTCAACGAGTCGGCGCACAAGAACGTGCTGATGAACCTCGCCCTGAACCTCAAGGCGGTGGTGTCGCAGCG
CCTGGTGGTCGGCACCGATGGCCGCCGACTGCCCGCGGCCGAGATCCTGATCAACACCCCGCACGTGCGCGACCTGATGC
GCCGTGGCCAGGTGCACGAGATCAAGCAGGCGATGGAGGAGTCGCTGGAGGAAGGCATGGAGTCGTTCGACCAATGCCTG
TTCCGGCTCTACAAGGAAGGCCGCATCGAGATGGAGGAAGCGCTCAAGGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGCGCCGAGCACGATCCTTACGCGGACATGTACGACGCCAACGCCGCGCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q8CXP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

97.027

0.5
  pilU Acinetobacter baylyi ADP1

49.153

95.676

0.47
  pilU Vibrio cholerae strain A1552

47.458

95.676

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.205

98.649

0.446
  pilT Pseudomonas aeruginosa PAK

41.124

91.351

0.376
  pilT Acinetobacter baumannii D1279779

40.828

91.351

0.373
  pilT Acinetobacter baumannii strain A118

40.828

91.351

0.373
  pilT Acinetobacter nosocomialis M2

40.828

91.351

0.373
  pilT Acinetobacter baylyi ADP1

40.294

91.892

0.37
  pilT Pseudomonas stutzeri DSM 10701

40.237

91.351

0.368
  pilT Legionella pneumophila strain Lp02

40.237

91.351

0.368
  pilT Legionella pneumophila strain ERS1305867

40.237

91.351

0.368
  pilT Neisseria gonorrhoeae MS11

38.905

93.784

0.365
  pilT Neisseria meningitidis 8013

38.905

93.784

0.365