Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DYST_RS10475 Genome accession   NZ_CP089414
Coordinates   2443181..2444296 (+) Length   371 a.a.
NCBI ID   WP_199178908.1    Uniprot ID   -
Organism   Dyella terrae strain Ely Copper Mine     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2438181..2449296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DYST_RS10455 (DYST_02144) - 2439725..2441095 (-) 1371 WP_102302016.1 pyridoxal-phosphate dependent enzyme -
  DYST_RS10460 (DYST_02145) - 2441252..2441479 (-) 228 WP_102302015.1 YdcH family protein -
  DYST_RS10465 (DYST_02146) - 2441889..2442482 (-) 594 WP_102302165.1 hypothetical protein -
  DYST_RS10470 (DYST_02147) - 2442503..2443099 (-) 597 WP_102302014.1 DUF4398 domain-containing protein -
  DYST_RS10475 (DYST_02148) pilU 2443181..2444296 (+) 1116 WP_199178908.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DYST_RS10480 (DYST_02149) - 2444306..2444686 (-) 381 WP_239951733.1 hypothetical protein -
  DYST_RS10485 (DYST_02150) maiA 2444691..2445359 (-) 669 WP_239951734.1 maleylacetoacetate isomerase -
  DYST_RS10490 (DYST_02151) - 2445497..2446675 (+) 1179 WP_239951737.1 phosphoglycerate kinase -
  DYST_RS10495 (DYST_02152) - 2446675..2447307 (+) 633 WP_239951738.1 HAD hydrolase-like protein -
  DYST_RS10500 (DYST_02153) - 2447369..2447917 (-) 549 WP_239951740.1 DinB family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41369.51 Da        Isoelectric Point: 6.3522

>NTDB_id=636863 DYST_RS10475 WP_199178908.1 2443181..2444296(+) (pilU) [Dyella terrae strain Ely Copper Mine]
MDIGYFLKLMVDKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPSGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEVGMVIRAIKSEIPSIDQLQLPTIFRELIMEPRGLILVVGATGSGKSTTLAAMIDHRNQNSSGHILTIED
PIEYLHRHKKSIVNQREVGLDTHSYHEALRNAMREAPDVIMIGEIRDTDTMEAAISFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVVGKDGRRLPATEVLLNTPLIRDMIRRGQVHDVKEAMDRSLQEGMQTFDQSL
YRLYKEGRIELDEALNKADSRDGLALKIRLSDSGNTESTELVGNDPYGLGF

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=636863 DYST_RS10475 WP_199178908.1 2443181..2444296(+) (pilU) [Dyella terrae strain Ely Copper Mine]
GTGGACATCGGTTATTTCCTCAAGCTAATGGTAGACAAGGGCGCGTCGGATATGTTCCTGACGACGGGCGCCCCTGTGAA
CATTAAGGTCGAGGGCAAGCTTTACCCATTGGGCAATACTGGCCTGCCTAGTGGCATGGTCAAGAAAATCGCCTACTCGC
TGATGGACGAGGGCCAGGTACCCCAGTTCGAGCGCGACCTGGAGTTGAACATGGCGCTCGCCGTGAAGGAAGCCGGACGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGTGCCATCAAGAGTGAAATTCCCAGCATCGA
TCAGTTGCAGCTGCCGACCATTTTCCGCGAACTCATCATGGAACCGCGCGGCCTGATCCTGGTGGTGGGTGCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCGCCATGATCGATCACCGCAATCAGAACTCATCGGGCCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGCCTGGATACGCACAGCTATCACGA
AGCCCTGCGCAACGCGATGCGCGAGGCGCCTGACGTGATCATGATTGGCGAGATCCGCGACACGGACACGATGGAAGCCG
CCATTTCATTTTCCGAAACCGGCCATCTCTGCCTTGCCACGCTGCATTCGAACAACGCTGACCAGACGTTGGAGCGAATC
CTCAACTTCTTCCCGGAATCGGCCCACAAGAACGTGTTGATGAACCTCGCGCTCAATCTGCGTGCGGTGATCAGCCAGCG
TCTCGTGGTCGGCAAGGACGGCCGCCGCCTGCCAGCGACGGAAGTGCTGCTGAACACGCCACTGATCCGCGACATGATCC
GCCGCGGCCAGGTGCACGACGTCAAGGAAGCGATGGACCGCAGCCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGCTG
TACCGCCTCTACAAGGAAGGCCGCATCGAGCTGGACGAGGCACTCAACAAGGCCGACTCCCGCGATGGGTTGGCGCTCAA
GATTCGCCTGTCCGACAGCGGCAACACCGAAAGCACCGAACTGGTCGGCAACGACCCCTACGGCCTGGGCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.838

96.496

0.491
  pilU Acinetobacter baylyi ADP1

50.14

96.226

0.482
  pilU Vibrio cholerae strain A1552

49.429

94.34

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.133

91.375

0.412
  pilT Acinetobacter baylyi ADP1

42.059

91.644

0.385
  pilT Pseudomonas stutzeri DSM 10701

41.716

91.105

0.38
  pilT Acinetobacter nosocomialis M2

41.716

91.105

0.38
  pilT Acinetobacter baumannii D1279779

41.716

91.105

0.38
  pilT Acinetobacter baumannii strain A118

41.716

91.105

0.38
  pilT Legionella pneumophila strain ERS1305867

41.228

92.183

0.38
  pilT Legionella pneumophila strain Lp02

41.228

92.183

0.38
  pilT Pseudomonas aeruginosa PAK

41.42

91.105

0.377
  pilT Vibrio cholerae strain A1552

40.356

90.836

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.356

90.836

0.367