Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LSQ66_RS01265 Genome accession   NZ_CP088952
Coordinates   264550..265728 (+) Length   392 a.a.
NCBI ID   WP_269449127.1    Uniprot ID   -
Organism   Massilia endophytica strain DM-R-R2A-13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 259550..270728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSQ66_RS01240 (LSQ66_01240) - 260521..261228 (+) 708 WP_231768007.1 YggS family pyridoxal phosphate-dependent enzyme -
  LSQ66_RS01245 (LSQ66_01245) proC 261231..262079 (+) 849 WP_231768008.1 pyrroline-5-carboxylate reductase -
  LSQ66_RS01250 (LSQ66_01250) - 262194..262559 (-) 366 WP_231768009.1 YqjD family protein -
  LSQ66_RS01255 (LSQ66_01255) ubiA 262598..263473 (-) 876 WP_231768010.1 4-hydroxybenzoate octaprenyltransferase -
  LSQ66_RS01260 (LSQ66_01260) - 263507..264448 (-) 942 WP_231768011.1 hydrogen peroxide-inducible genes activator -
  LSQ66_RS01265 (LSQ66_01265) pilU 264550..265728 (+) 1179 WP_269449127.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LSQ66_RS01270 (LSQ66_01270) - 265725..266207 (+) 483 WP_231768012.1 adenylyltransferase/cytidyltransferase family protein -
  LSQ66_RS01275 (LSQ66_01275) - 266210..266689 (-) 480 WP_231768013.1 heme-binding protein -
  LSQ66_RS01280 (LSQ66_01280) - 266729..267187 (-) 459 WP_231768014.1 DUF421 domain-containing protein -
  LSQ66_RS01285 (LSQ66_01285) - 267315..268061 (-) 747 WP_231768015.1 transposase -
  LSQ66_RS01290 (LSQ66_01290) trmL 268181..268651 (-) 471 WP_231768016.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  LSQ66_RS01295 (LSQ66_01295) - 268662..269393 (-) 732 WP_231768017.1 ComF family protein -
  LSQ66_RS01300 (LSQ66_01300) - 269452..270378 (+) 927 WP_231768018.1 methyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 43594.63 Da        Isoelectric Point: 6.1506

>NTDB_id=634097 LSQ66_RS01265 WP_269449127.1 264550..265728(+) (pilU) [Massilia endophytica strain DM-R-R2A-13]
MSLTPDEAQAYIHKLLKAMHQVGGSDLFISSDFPPSIKSQGSMRALSQQRLTGDVTRALAYALMNERQRAEFEAEMECNF
AISLPDVCRFRVNVFVQQQNVGMVVRTIASEIPNFEKLDLPDVLKDVIMTKRGLVLVVGGTGSGKSTTLAAMIDYRNSNS
AGHIITVEDPVEYVHKNKGCLITHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLHAN
NANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLIRTEDGKGRKAAIEILLNTPTIAEMIFKGNFHGIKEIMHKSRE
LGMCTFDQALYELYNKGYISYEEALRNADSTNGLRLQIKLHGDRKEPGGAAAAASASLSMQVDEEDEEEPGT

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=634097 LSQ66_RS01265 WP_269449127.1 264550..265728(+) (pilU) [Massilia endophytica strain DM-R-R2A-13]
ATGAGTCTCACTCCCGACGAAGCCCAGGCTTACATCCACAAACTGCTGAAGGCGATGCACCAGGTGGGCGGTTCGGACCT
GTTCATCTCGTCCGATTTTCCGCCAAGCATTAAATCGCAGGGCTCGATGCGGGCCCTGAGCCAGCAGCGCCTGACGGGCG
ACGTTACGCGGGCCCTGGCCTACGCCCTGATGAACGAGCGCCAGCGCGCCGAGTTCGAAGCCGAGATGGAATGTAACTTC
GCCATCTCCCTGCCGGATGTGTGCCGATTCCGCGTGAACGTCTTCGTACAGCAGCAAAACGTGGGCATGGTAGTGAGGAC
CATCGCCTCCGAGATTCCGAATTTCGAAAAGCTGGACCTGCCCGACGTGCTGAAGGACGTCATTATGACCAAGCGCGGCC
TGGTGCTGGTAGTGGGCGGCACGGGCTCGGGCAAGTCGACCACGCTGGCCGCCATGATCGACTACCGCAACAGCAATTCG
GCGGGGCACATCATCACCGTCGAAGACCCGGTGGAGTACGTCCACAAGAACAAGGGCTGCCTGATCACGCACCGCGAGGT
GGGCGTCGATACGCATTCGTGGCACCACGCGCTGAAGAACACGCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAAA
TCCGAGACACGGAAACCATGGAACACGCGATTGCGTTTGCCGAGACCGGACACCTCTGCCTGGGCACCCTGCACGCGAAC
AACGCGAACCAGACGATGGACCGCATCATCAATTTCTTCCCGGAGGAGCGGCGCAACCAGCTGCTGATGGACCTCTCCTC
GAACCTGCGCGCCATTGTGTCGCAGCGCCTGATCCGCACCGAGGACGGCAAGGGGCGGAAGGCCGCGATCGAAATCCTGC
TCAATACGCCGACGATTGCGGAGATGATCTTCAAGGGCAATTTCCACGGCATCAAGGAGATCATGCACAAATCGCGGGAG
CTGGGCATGTGCACCTTCGACCAGGCCCTGTACGAGCTTTATAATAAGGGCTACATCTCCTACGAAGAAGCGCTGCGCAA
CGCGGACTCCACGAACGGGCTGCGCCTGCAGATCAAGCTGCACGGCGACCGCAAGGAACCGGGCGGCGCGGCGGCGGCCG
CCTCTGCATCGCTCAGCATGCAGGTGGACGAAGAAGACGAAGAAGAACCAGGCACATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.878

96.429

0.548
  pilU Acinetobacter baylyi ADP1

53.39

90.306

0.482
  pilU Vibrio cholerae strain A1552

52.299

88.776

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

85.969

0.38
  pilT Acinetobacter baylyi ADP1

43.658

86.48

0.378
  pilT Pseudomonas aeruginosa PAK

43.917

85.969

0.378
  pilT Acinetobacter baumannii strain A118

43.844

84.949

0.372
  pilT Acinetobacter nosocomialis M2

43.844

84.949

0.372
  pilT Acinetobacter baumannii D1279779

43.844

84.949

0.372
  pilT Legionella pneumophila strain ERS1305867

45.483

81.888

0.372
  pilT Legionella pneumophila strain Lp02

45.483

81.888

0.372
  pilT Pseudomonas stutzeri DSM 10701

43.027

85.969

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.771

84.694

0.362
  pilT Vibrio cholerae strain A1552

42.771

84.694

0.362