Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LRK32_RS02760 Genome accession   NZ_CP088920
Coordinates   613390..614502 (-) Length   370 a.a.
NCBI ID   WP_015446779.1    Uniprot ID   M4NAW7
Organism   Rhodanobacter denitrificans strain FW104-10F02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 608390..619502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRK32_RS02740 (LRK32_02740) tkt 608547..610556 (+) 2010 WP_007512519.1 transketolase -
  LRK32_RS02745 (LRK32_02745) - 610660..611298 (-) 639 WP_027485749.1 HAD hydrolase-like protein -
  LRK32_RS02750 (LRK32_02750) - 611298..612482 (-) 1185 WP_007512516.1 phosphoglycerate kinase -
  LRK32_RS02755 (LRK32_02755) maiA 612654..613322 (+) 669 WP_007512514.1 maleylacetoacetate isomerase -
  LRK32_RS02760 (LRK32_02760) pilU 613390..614502 (-) 1113 WP_015446779.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LRK32_RS02765 (LRK32_02765) - 614589..615146 (+) 558 WP_015446780.1 DUF4398 domain-containing protein -
  LRK32_RS02770 (LRK32_02770) - 615143..615784 (+) 642 WP_027485752.1 hypothetical protein -
  LRK32_RS02775 (LRK32_02775) - 616139..616366 (+) 228 WP_007510956.1 YdcH family protein -
  LRK32_RS02780 (LRK32_02780) - 616523..617893 (+) 1371 WP_027489962.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41027.26 Da        Isoelectric Point: 6.2494

>NTDB_id=633940 LRK32_RS02760 WP_015446779.1 613390..614502(-) (pilU) [Rhodanobacter denitrificans strain FW104-10F02]
MDIGYFLKLMVDKGASDMFLSTGAPVNIKVEGKLYPLGNTGLPSGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEVGMVIRAIKSEIPSIDQLQLPGIFRDLIMEPRGLILVVGATGSGKSTTLAAMLDQRNTNSPGHILTIED
PIEYLHRHKKSIVNQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTDTMEAAIAFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVLGKDGRRIPAAEVLLNTPLIRDMIRRGQIHEIKEAMDRSLQEGMQTFDQSL
YKLYKEGRVELEEALNKSDSRDGLALKIRLAEGGSSDEALAGNDPYGMGF

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=633940 LRK32_RS02760 WP_015446779.1 613390..614502(-) (pilU) [Rhodanobacter denitrificans strain FW104-10F02]
GTGGATATCGGCTACTTCCTCAAGCTTATGGTAGACAAGGGCGCGTCGGACATGTTCCTGTCCACCGGCGCCCCGGTGAA
TATCAAGGTCGAGGGCAAGCTGTACCCGCTGGGCAACACCGGCCTGCCCAGCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTACCGCAGTTCGAACGCGACCTGGAACTGAACATGGCGCTGGCGGTGAAGGAAGCCGGCCGT
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCGATCAAGAGCGAGATCCCCAGCATCGA
CCAGCTGCAGTTGCCCGGCATCTTCCGCGACCTGATCATGGAGCCGCGCGGCCTGATCCTGGTGGTCGGCGCCACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGCTCGACCAGCGCAACACCAATTCGCCCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTGGGGCTGGACACGCACAGCTACCACGA
AGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCGACACCGACACGATGGAGGCGG
CGATCGCGTTCTCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCCGAGTCGGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCGGTGATCAGCCAGCG
CCTGGTACTCGGCAAGGACGGCCGCCGCATCCCGGCCGCCGAGGTGTTGCTGAACACCCCGCTGATCCGCGACATGATCC
GCCGCGGCCAGATCCACGAGATCAAGGAAGCGATGGACCGCAGCCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGCTG
TACAAGCTGTACAAGGAAGGCCGCGTGGAGCTGGAGGAAGCGCTCAACAAGTCCGATTCGCGCGACGGCCTGGCGCTGAA
GATCCGCCTGGCCGAAGGCGGGTCCAGCGACGAGGCGCTGGCTGGCAACGACCCGTACGGCATGGGGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M4NAW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.559

96.757

0.489
  pilU Vibrio cholerae strain A1552

47.568

100

0.476
  pilU Acinetobacter baylyi ADP1

49.435

95.676

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

91.622

0.405
  pilT Acinetobacter baylyi ADP1

42.216

90.27

0.381
  pilT Legionella pneumophila strain Lp02

41.228

92.432

0.381
  pilT Legionella pneumophila strain ERS1305867

41.228

92.432

0.381
  pilT Acinetobacter baumannii D1279779

41.916

90.27

0.378
  pilT Acinetobacter nosocomialis M2

41.916

90.27

0.378
  pilT Acinetobacter baumannii strain A118

41.916

90.27

0.378
  pilT Pseudomonas aeruginosa PAK

41.124

91.351

0.376
  pilT Pseudomonas stutzeri DSM 10701

40.828

91.351

0.373
  pilT Vibrio cholerae strain A1552

40.356

91.081

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.356

91.081

0.368