Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GKIL_RS10585 Genome accession   NC_022600
Coordinates   2287309..2288412 (-) Length   367 a.a.
NCBI ID   WP_023173565.1    Uniprot ID   U5QHH3
Organism   Gloeobacter kilaueensis JS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2282309..2293412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKIL_RS10560 (GKIL_2166) glpX 2283290..2284330 (+) 1041 WP_023173557.1 class II fructose-bisphosphatase -
  GKIL_RS10565 (GKIL_2167) - 2284450..2285022 (+) 573 WP_023173558.1 Uma2 family endonuclease -
  GKIL_RS10570 (GKIL_2168) - 2285121..2285483 (+) 363 WP_023173560.1 hypothetical protein -
  GKIL_RS10575 (GKIL_2169) - 2285499..2286035 (+) 537 WP_023173562.1 RES family NAD+ phosphorylase -
  GKIL_RS10580 (GKIL_2170) - 2286060..2287292 (-) 1233 WP_023173564.1 type II secretion system F family protein -
  GKIL_RS10585 (GKIL_2171) pilT 2287309..2288412 (-) 1104 WP_023173565.1 type IV pilus twitching motility protein PilT Machinery gene
  GKIL_RS10590 (GKIL_2172) pilB/pilB1 2288492..2290150 (-) 1659 WP_023173567.1 GspE/PulE family protein Machinery gene
  GKIL_RS10595 (GKIL_2173) - 2290314..2291087 (+) 774 WP_023173568.1 sigma-70 family RNA polymerase sigma factor -
  GKIL_RS10600 (GKIL_2174) rpaB 2291277..2292002 (+) 726 WP_041244642.1 response regulator transcription factor RpaB -
  GKIL_RS10605 (GKIL_2175) - 2292082..2292774 (+) 693 WP_023173570.1 hypothetical protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 39825.50 Da        Isoelectric Point: 6.6125

>NTDB_id=63024 GKIL_RS10585 WP_023173565.1 2287309..2288412(-) (pilT) [Gloeobacter kilaueensis JS1]
MPVAIETLMQHVIDSAGSDLHIAAGTPPCIRVGGALHFVGNEPLSPADSEALILPLLSNNQRLQLEQNWELDCSYSHGGT
RFRVNVYKDKGNYAAALRALSSRIPTIQELGLPPVMREIADRPRGLVLVTGPTGSGKTTTLASLVDYINGSRSEHILTVE
DPIEYLFVPKRSLINQRQLGEDTKSFANALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFATLHTSSAAQTVDR
MVDVFPPAQQQQIRVQLSNSLVAVFSQTLIPRVQAGRGLQSRVMAQEIMVVNPAIANLIREGKTSQIYSALQTGGSLGMK
TLESSLRDLVTSGQIALENALTYTSRPEEFQRMLGGAAPRPATSRPY

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=63024 GKIL_RS10585 WP_023173565.1 2287309..2288412(-) (pilT) [Gloeobacter kilaueensis JS1]
ATGCCCGTAGCCATTGAAACGCTGATGCAGCACGTCATCGACTCGGCGGGTTCAGACCTGCACATTGCCGCCGGTACGCC
CCCCTGCATCCGGGTGGGCGGTGCGCTGCACTTCGTGGGCAACGAGCCTCTGAGTCCCGCCGACTCCGAGGCGCTCATCC
TGCCGCTACTCAGCAACAACCAGCGGCTGCAGCTTGAGCAGAACTGGGAATTGGACTGCTCCTACAGCCACGGCGGCACC
CGCTTTCGCGTCAATGTCTATAAAGACAAGGGCAACTACGCCGCAGCTCTGCGCGCCCTCAGCTCCCGCATCCCGACGAT
CCAGGAACTGGGCCTGCCGCCGGTCATGCGCGAGATCGCCGATCGGCCCCGTGGGCTGGTGCTCGTCACCGGTCCCACCG
GCTCCGGCAAGACGACCACCCTCGCCTCCCTCGTCGATTACATCAACGGCAGCCGCTCCGAGCACATCCTCACCGTCGAG
GATCCGATCGAATATCTGTTCGTTCCCAAGCGCTCCCTCATCAACCAGCGCCAGTTGGGCGAAGACACCAAAAGCTTTGC
CAACGCCCTGCGCGCCGCCCTGCGCGAAGATCCCGACGTGATCTTAGTCGGCGAGATGCGCGACCTTGAGACGATTCAAC
TGGCGATCACCGCCGCTGAGACCGGCCACCTCGTCTTTGCGACCCTGCACACCAGCTCCGCCGCCCAGACAGTCGATCGC
ATGGTCGATGTCTTCCCGCCCGCCCAGCAGCAGCAGATCCGCGTCCAGCTCTCCAATTCGCTGGTCGCCGTCTTCTCCCA
GACCTTGATCCCGAGGGTGCAGGCGGGCCGGGGACTGCAGTCGCGGGTGATGGCCCAGGAGATCATGGTTGTCAACCCCG
CCATCGCCAACCTCATCCGCGAGGGCAAGACCAGCCAGATCTACTCTGCCCTGCAGACCGGCGGCAGCCTCGGAATGAAG
ACGCTCGAAAGCTCCCTGCGCGACCTGGTCACCTCCGGTCAGATCGCCCTCGAAAACGCCCTTACCTACACCTCCCGGCC
CGAAGAATTCCAGCGCATGTTGGGAGGAGCCGCTCCCCGGCCTGCCACCAGCCGCCCCTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U5QHH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.728

100

0.499
  pilT Pseudomonas aeruginosa PAK

51.873

94.55

0.49
  pilT Acinetobacter baylyi ADP1

51.585

94.55

0.488
  pilT Acinetobacter baumannii D1279779

50.72

94.55

0.48
  pilT Acinetobacter baumannii strain A118

50.72

94.55

0.48
  pilT Pseudomonas stutzeri DSM 10701

50.72

94.55

0.48
  pilT Acinetobacter nosocomialis M2

50.72

94.55

0.48
  pilT Legionella pneumophila strain Lp02

50.742

91.826

0.466
  pilT Legionella pneumophila strain ERS1305867

50.742

91.826

0.466
  pilT Neisseria meningitidis 8013

51.376

89.101

0.458
  pilT Neisseria gonorrhoeae MS11

51.07

89.101

0.455
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.494

90.463

0.439
  pilT Vibrio cholerae strain A1552

48.494

90.463

0.439

Multiple sequence alignment