Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GKIL_RS10590 Genome accession   NC_022600
Coordinates   2288492..2290150 (-) Length   552 a.a.
NCBI ID   WP_023173567.1    Uniprot ID   U5QHS7
Organism   Gloeobacter kilaueensis JS1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2283492..2295150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKIL_RS10565 (GKIL_2167) - 2284450..2285022 (+) 573 WP_023173558.1 Uma2 family endonuclease -
  GKIL_RS10570 (GKIL_2168) - 2285121..2285483 (+) 363 WP_023173560.1 hypothetical protein -
  GKIL_RS10575 (GKIL_2169) - 2285499..2286035 (+) 537 WP_023173562.1 RES family NAD+ phosphorylase -
  GKIL_RS10580 (GKIL_2170) - 2286060..2287292 (-) 1233 WP_023173564.1 type II secretion system F family protein -
  GKIL_RS10585 (GKIL_2171) pilT 2287309..2288412 (-) 1104 WP_023173565.1 type IV pilus twitching motility protein PilT Machinery gene
  GKIL_RS10590 (GKIL_2172) pilB/pilB1 2288492..2290150 (-) 1659 WP_023173567.1 GspE/PulE family protein Machinery gene
  GKIL_RS10595 (GKIL_2173) - 2290314..2291087 (+) 774 WP_023173568.1 sigma-70 family RNA polymerase sigma factor -
  GKIL_RS10600 (GKIL_2174) rpaB 2291277..2292002 (+) 726 WP_041244642.1 response regulator transcription factor RpaB -
  GKIL_RS10605 (GKIL_2175) - 2292082..2292774 (+) 693 WP_023173570.1 hypothetical protein -
  GKIL_RS25620 (GKIL_2176) - 2293105..2294928 (+) 1824 WP_023173571.1 IPT/TIG domain-containing protein -

Sequence

Protein


Download         Length: 552 a.a.        Molecular weight: 60990.03 Da        Isoelectric Point: 6.8087

>NTDB_id=63025 GKIL_RS10590 WP_023173567.1 2288492..2290150(-) (pilB/pilB1) [Gloeobacter kilaueensis JS1]
MTALALSERTLATHQRLFEIKLLFGVMVIDPEQDAPGEAESGRLRELVGSEACAHYRLLPIRQEQSTVVVAMVDPDDLEA
IDHLVRRLHCQGLALRRVAVGEEEYGRWLARRGVGEERAVLPEWQVQEESPVLRLAGQILLRAISSGSSDIHIEPTERHL
RIRLRQDGVLREAFEPLPIEIAPALVSHFKVLAELDIAERRLPQDGRLRRHFDGRAIDFRLSTLPGRYGEKLVLRLLDSA
ARFRLAELIADGQTLALVNRWIHRPHGLVLVTGPTGSGKTTTLYAALEERNEVGLNIVTAEDPIEYSLPGVTQVQVLREK
GLDFARILRAFLRQDPDILLVGESRDRETIATAVEAALTGHLVLTSLHTNDAAEAIVRLAELGIEPYLITGALVGVLAQR
LVRRLCAHCREPYYPNPERLRPFGALTATTGKVVFYRARSCDHCGGTGYKGRVGVYEAMGISEGLRTLIADGASAARLRG
AALEAGMQTLLTSALQLARRGLTSLEEVERVVLTDAGLHACTAHTSNCRGCRAELRPEWIDCPYCTTPRQDG

Nucleotide


Download         Length: 1659 bp        

>NTDB_id=63025 GKIL_RS10590 WP_023173567.1 2288492..2290150(-) (pilB/pilB1) [Gloeobacter kilaueensis JS1]
ATGACGGCTCTTGCATTGAGCGAGCGGACCCTGGCAACGCACCAGCGGTTATTTGAAATCAAGCTGCTTTTTGGGGTGAT
GGTGATCGATCCCGAGCAGGACGCGCCGGGCGAGGCAGAATCCGGACGGCTGCGGGAACTCGTCGGGTCCGAAGCCTGTG
CCCATTACCGGTTGCTCCCGATCCGGCAGGAGCAGTCCACCGTAGTCGTCGCGATGGTCGATCCAGATGATCTGGAGGCC
ATCGACCATCTCGTGCGCCGCCTGCACTGCCAGGGACTTGCCCTGAGGCGGGTGGCGGTGGGCGAGGAGGAGTACGGGCG
CTGGCTTGCCCGGCGGGGGGTCGGGGAGGAACGGGCGGTGTTGCCGGAGTGGCAGGTGCAGGAGGAAAGCCCGGTTCTCC
GGCTCGCGGGCCAGATTCTTTTGCGGGCAATCAGCAGCGGCAGCTCCGACATCCATATCGAACCGACTGAGAGGCACCTG
CGCATCCGTCTGCGCCAGGACGGCGTGCTGCGCGAGGCGTTCGAGCCCTTGCCGATTGAGATCGCCCCGGCTCTGGTGTC
GCACTTCAAGGTGCTGGCGGAACTGGACATTGCCGAGCGGCGGTTGCCCCAGGATGGCCGCCTGCGCCGCCACTTCGATG
GCCGGGCGATCGACTTTCGCCTCTCGACGCTGCCGGGACGCTACGGCGAAAAGCTGGTATTGCGCCTGTTAGACAGTGCC
GCTCGCTTTCGCCTGGCAGAACTCATCGCGGATGGGCAGACTCTGGCGCTCGTCAACCGCTGGATCCACCGCCCCCACGG
CCTGGTGCTCGTCACCGGCCCCACCGGCTCGGGCAAGACGACGACGCTCTACGCCGCCCTGGAGGAGCGCAACGAAGTGG
GCCTCAACATCGTCACCGCTGAAGATCCGATCGAGTACAGCCTGCCGGGGGTGACCCAGGTGCAGGTGCTGCGCGAGAAG
GGGCTCGACTTTGCCCGGATCCTGCGCGCCTTCTTGCGCCAGGATCCGGACATCTTGCTGGTGGGTGAAAGCCGCGACCG
CGAGACGATCGCCACCGCCGTCGAGGCGGCCCTCACCGGGCATCTGGTGCTCACCAGCCTGCACACCAACGACGCCGCCG
AAGCGATCGTCCGGCTGGCAGAACTGGGTATCGAGCCGTACCTCATCACCGGTGCGCTGGTGGGTGTGCTGGCCCAGCGG
CTGGTGCGCCGCCTCTGTGCCCACTGCCGCGAACCGTACTATCCGAACCCTGAGCGGCTCAGACCGTTCGGCGCGCTCAC
AGCCACCACCGGCAAAGTCGTCTTCTACCGCGCCCGGAGCTGTGATCACTGCGGCGGCACCGGCTACAAGGGCCGGGTCG
GCGTCTACGAAGCGATGGGTATCAGCGAAGGGCTGCGCACACTGATTGCCGACGGCGCTTCTGCAGCTCGCCTGCGGGGA
GCGGCGCTTGAGGCAGGAATGCAGACGCTGCTCACCTCCGCGCTGCAACTGGCCCGCCGGGGGCTTACGAGCCTCGAAGA
AGTCGAGCGGGTCGTCCTCACCGATGCGGGTTTGCACGCCTGCACCGCCCATACCAGTAACTGCCGGGGTTGCCGGGCCG
AGTTGCGGCCCGAGTGGATCGACTGCCCGTACTGCACAACGCCCCGCCAGGATGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(10-112)

Type II/IV secretion system protein (T2SSE)

(138-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U5QHS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

48.27

100

0.531
  pilB Vibrio cholerae strain A1552

40.968

97.283

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.116

90.761

0.382
  pilF Thermus thermophilus HB27

41.502

91.667

0.38
  pilB Vibrio campbellii strain DS40M4

41.057

89.13

0.366
  pilB Vibrio parahaemolyticus RIMD 2210633

40.447

89.13

0.361

Multiple sequence alignment