Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   LOY41_RS22610 Genome accession   NZ_CP087187
Coordinates   5036312..5036734 (-) Length   140 a.a.
NCBI ID   WP_041477820.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain B21-026     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5031312..5041734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY41_RS22595 (LOY41_22560) - 5032179..5035130 (+) 2952 WP_122706076.1 DUF748 domain-containing protein -
  LOY41_RS22600 (LOY41_22565) - 5035127..5035426 (+) 300 WP_039761633.1 DUF2845 domain-containing protein -
  LOY41_RS22605 (LOY41_22570) - 5035662..5036015 (-) 354 WP_016773155.1 BON domain-containing protein -
  LOY41_RS22610 (LOY41_22575) pilA/pilAI 5036312..5036734 (-) 423 WP_041477820.1 pilin Machinery gene
  LOY41_RS22615 (LOY41_22580) pilB 5036961..5038661 (+) 1701 WP_073475466.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY41_RS22620 (LOY41_22585) pilC 5038664..5039881 (+) 1218 WP_024014342.1 type II secretion system F family protein Machinery gene
  LOY41_RS22625 (LOY41_22590) pilD 5039883..5040755 (+) 873 WP_122698545.1 A24 family peptidase Machinery gene
  LOY41_RS22630 (LOY41_22595) coaE 5040752..5041375 (+) 624 WP_122706074.1 dephospho-CoA kinase -
  LOY41_RS22635 (LOY41_22600) yacG 5041372..5041572 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14144.32 Da        Isoelectric Point: 8.4918

>NTDB_id=627637 LOY41_RS22610 WP_041477820.1 5036312..5036734(-) (pilA/pilAI) [Pseudomonas atacamensis strain B21-026]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDLINQGTNPSLAAVKGDNVTTTSNCA
LTASGTAADGAGTIVCTLANAPAPVLGKTITLTRTAAGGWSCGTTVAPDYSPKGCNATGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=627637 LOY41_RS22610 WP_041477820.1 5036312..5036734(-) (pilA/pilAI) [Pseudomonas atacamensis strain B21-026]
ATGAAAAAACAACAAGGTTTCACATTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGCATTCTGGCGACTATCGCCCT
GCCGCAGTATTCGAAGTATCAGGCGCGGTCGAAGGTGACGGCGGGTTTGGCGGAGATCAGCGCGATGAAGGTGCCGTTTG
AAGACCTGATCAATCAGGGGACAAATCCAAGCTTGGCGGCGGTGAAAGGCGATAACGTCACGACGACCTCCAATTGCGCT
CTCACCGCGTCAGGCACTGCTGCTGATGGGGCTGGAACGATTGTATGTACGCTGGCCAACGCGCCAGCGCCTGTATTAGG
CAAAACCATCACGCTGACTCGTACAGCTGCGGGTGGTTGGAGCTGTGGCACTACGGTAGCTCCTGACTATTCGCCGAAAG
GCTGTAACGCAACTGGTACTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.365

97.857

0.493
  pilA Acinetobacter baumannii strain A118

46.763

99.286

0.464
  pilA Vibrio cholerae strain A1552

41.29

100

0.457
  pilA Vibrio cholerae C6706

41.29

100

0.457
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.29

100

0.457
  comP Acinetobacter baylyi ADP1

41.447

100

0.45
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.443
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA2 Legionella pneumophila str. Paris

39.041

100

0.407
  pilA Vibrio parahaemolyticus RIMD 2210633

42.222

96.429

0.407
  pilA2 Legionella pneumophila strain ERS1305867

36.986

100

0.386
  pilA/pilA1 Eikenella corrodens VA1

39.259

96.429

0.379