Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   LOY56_RS22395 Genome accession   NZ_CP087168
Coordinates   4789210..4789620 (-) Length   136 a.a.
NCBI ID   WP_258617155.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4784210..4794620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY56_RS22380 (LOY56_22330) - 4785069..4788047 (+) 2979 WP_258617152.1 DUF748 domain-containing protein -
  LOY56_RS22385 (LOY56_22335) - 4788047..4788337 (+) 291 WP_258617153.1 DUF2845 domain-containing protein -
  LOY56_RS22390 (LOY56_22340) - 4788539..4788895 (-) 357 WP_258617154.1 BON domain-containing protein -
  LOY56_RS22395 (LOY56_22345) pilA/pilAI 4789210..4789620 (-) 411 WP_258617155.1 pilin Machinery gene
  LOY56_RS22400 (LOY56_22350) pilB 4789847..4791547 (+) 1701 WP_258617156.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY56_RS22405 (LOY56_22355) pilC 4791550..4792767 (+) 1218 WP_258617157.1 type II secretion system F family protein Machinery gene
  LOY56_RS22410 (LOY56_22360) pilD 4792769..4793641 (+) 873 WP_258617158.1 A24 family peptidase Machinery gene
  LOY56_RS22415 (LOY56_22365) coaE 4793638..4794261 (+) 624 WP_258617159.1 dephospho-CoA kinase -
  LOY56_RS22420 (LOY56_22370) yacG 4794258..4794458 (+) 201 WP_258617160.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13817.99 Da        Isoelectric Point: 8.1087

>NTDB_id=627157 LOY56_RS22395 WP_258617155.1 4789210..4789620(-) (pilA/pilAI) [Pseudomonas sp. B21-048]
MKNQNGFTLIELLIVVAIIGILATFALPQYSKYQSRAKVTAALAEISALKVPFEDIINQGTNPTLALIGGTLTTGNCTIT
ASGTASDGAGTIGCTILNAPGPVLSKTITLTRTLAGGWTCATTAAQEYAPKGCTGA

Nucleotide


Download         Length: 411 bp        

>NTDB_id=627157 LOY56_RS22395 WP_258617155.1 4789210..4789620(-) (pilA/pilAI) [Pseudomonas sp. B21-048]
ATGAAAAATCAAAATGGTTTTACTCTGATCGAACTGCTGATCGTGGTGGCGATCATCGGGATTTTGGCGACGTTTGCATT
GCCGCAGTATTCGAAGTACCAGTCGCGGGCGAAAGTCACTGCTGCGCTTGCAGAAATTTCGGCACTGAAAGTGCCTTTCG
AAGACATCATCAACCAGGGCACTAACCCCACCCTCGCACTGATCGGCGGTACGCTGACCACCGGTAATTGCACGATTACG
GCATCGGGTACCGCCTCTGACGGTGCAGGTACAATTGGTTGCACGATTCTCAACGCTCCAGGGCCGGTACTCAGCAAAAC
CATCACACTTACGCGCACGCTCGCCGGTGGGTGGACTTGCGCCACGACGGCTGCACAGGAATACGCGCCTAAAGGCTGCA
CTGGCGCCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.175

100

0.485
  pilA Acinetobacter baumannii strain A118

45.652

100

0.463
  pilA Vibrio cholerae strain A1552

43.056

100

0.456
  pilA Vibrio cholerae C6706

43.056

100

0.456
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.056

100

0.456
  comP Acinetobacter baylyi ADP1

40.132

100

0.449
  pilA Pseudomonas aeruginosa PAK

39.735

100

0.441
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

99.265

0.441
  pilA2 Legionella pneumophila str. Paris

38.889

100

0.412
  pilA Vibrio parahaemolyticus RIMD 2210633

38.971

100

0.39
  pilA2 Legionella pneumophila strain ERS1305867

37.681

100

0.382