Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOY56_RS22400 Genome accession   NZ_CP087168
Coordinates   4789847..4791547 (+) Length   566 a.a.
NCBI ID   WP_258617156.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-048     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4784847..4796547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY56_RS22380 (LOY56_22330) - 4785069..4788047 (+) 2979 WP_258617152.1 DUF748 domain-containing protein -
  LOY56_RS22385 (LOY56_22335) - 4788047..4788337 (+) 291 WP_258617153.1 DUF2845 domain-containing protein -
  LOY56_RS22390 (LOY56_22340) - 4788539..4788895 (-) 357 WP_258617154.1 BON domain-containing protein -
  LOY56_RS22395 (LOY56_22345) pilA/pilAI 4789210..4789620 (-) 411 WP_258617155.1 pilin Machinery gene
  LOY56_RS22400 (LOY56_22350) pilB 4789847..4791547 (+) 1701 WP_258617156.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY56_RS22405 (LOY56_22355) pilC 4791550..4792767 (+) 1218 WP_258617157.1 type II secretion system F family protein Machinery gene
  LOY56_RS22410 (LOY56_22360) pilD 4792769..4793641 (+) 873 WP_258617158.1 A24 family peptidase Machinery gene
  LOY56_RS22415 (LOY56_22365) coaE 4793638..4794261 (+) 624 WP_258617159.1 dephospho-CoA kinase -
  LOY56_RS22420 (LOY56_22370) yacG 4794258..4794458 (+) 201 WP_258617160.1 DNA gyrase inhibitor YacG -
  LOY56_RS22425 (LOY56_22375) - 4794468..4794683 (-) 216 WP_258617161.1 hypothetical protein -
  LOY56_RS22430 (LOY56_22380) - 4794747..4795436 (-) 690 WP_258617162.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62932.95 Da        Isoelectric Point: 5.8021

>NTDB_id=627158 LOY56_RS22400 WP_258617156.1 4789847..4791547(+) (pilB) [Pseudomonas sp. B21-048]
MNDIALSGLAKQLVLAELITDQSAQQAHQQAQRNRISLVSYLVQNKLLKSWQVAEVASEHFGMALLDLNCLDKDTQPRGL
VSEKLIRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSSGLNTEAILVEEDKLSDAIEKFFDNHSTGLEDMADVELDG
LDIESVDEQKQDPIAGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKIYRVRMRTDGMLREVARPPTQLANRIASRL
KVMASLDISERRKPQDGRIKMHLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINIEGINQVNVNARQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAQTLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDQCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=627158 LOY56_RS22400 WP_258617156.1 4789847..4791547(+) (pilB) [Pseudomonas sp. B21-048]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAACTCATCACTGATCAAAGTGCGCAACAGGC
GCATCAGCAAGCCCAACGCAATCGAATCTCGCTGGTCAGCTATCTGGTGCAAAACAAACTGCTGAAAAGCTGGCAGGTCG
CCGAGGTGGCTTCGGAGCATTTCGGCATGGCCCTGCTGGACCTCAACTGCCTGGATAAGGACACCCAACCCCGAGGACTG
GTCAGTGAGAAGCTGATCCGTCAGCACCATGCCCTGCCCCTCTGGCGGCGAGGTAACAAGCTATTCGTGGGGATTTCCGA
CCCGACCAATCATCAAGCCATCAACGACATCCAGTTCAGCTCCGGGCTGAACACCGAAGCCATTCTGGTCGAGGAAGACA
AGCTCAGCGACGCCATCGAAAAATTCTTCGACAACCACTCCACCGGCCTCGAAGACATGGCCGATGTCGAGCTCGACGGG
CTGGACATCGAGTCGGTCGATGAGCAGAAACAGGACCCCATTGCAGGGCAAGATACCGATGACGCGCCCGTGGTTCGCTT
CGTCCACAAGATGCTACTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTATGAAAAAATCTACCGCG
TGCGGATGCGCACCGACGGCATGCTGCGGGAGGTCGCCAGGCCGCCGACGCAACTGGCCAACCGCATTGCTTCCCGGCTG
AAGGTCATGGCCAGTCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGGCGAATCAAGATGCATCTGTCCAAAAGCAA
ATCCATCGACTTCCGGGTCAATACCCTGCCGACCCTGTGGGGCGAGAAGGTGGTGATCCGGATCCTCGACCCGTCCAGTG
CACAAATGGGCATCGATGCCCTCGGTTACGAACCCGAGCAAAAAGACCTGTACATGGCCGCCCTCAAGCAGCCACAGGGG
ATGATCCTGGTAACCGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTTTATACCGGGCTGAACATACTCAACACCGTCGA
CATCAATATTTCCACTGCCGAAGACCCGGTAGAAATCAACATCGAAGGCATCAACCAGGTCAACGTCAATGCCCGGCAAG
GACTGGATTTCGCCCAGGCGCTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGCGACCTC
GAAACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGCA
AACCCTGACCCGCTTGCACAACATGGGGATTCAGGGGTTCAACATCGCGACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGCGAGGCGTTGCTCAAAGAAGGCTTTCCCGAG
GAACGGATCGGCTCGTTCACGATCTATGAACCGGTCGGTTGCGATCAATGCAACGGCGGTTACAAAGGGCGCGTAGGGAT
TTATGAAGTGGTAAAAAATACTCCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAAATCGATATCCAGA
TGCGCAAGGACGGCTTCAATGACCTGCGTACTTCGGGCCTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.115

99.823

0.59
  pilB Acinetobacter baumannii D1279779

58.481

100

0.585
  pilB Legionella pneumophila strain ERS1305867

55.908

100

0.56
  pilB Vibrio cholerae strain A1552

50.088

99.823

0.5
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilB Vibrio campbellii strain DS40M4

47.895

100

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.273

97.173

0.479
  pilF Thermus thermophilus HB27

36.926

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.857

92.756

0.36