Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VIBNI_RS00320 Genome accession   NC_022528
Coordinates   58660..59766 (+) Length   368 a.a.
NCBI ID   WP_004398310.1    Uniprot ID   A0AAV2VQ18
Organism   Vibrio nigripulchritudo     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 53660..64766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIBNI_RS00285 (VIBNI_A0050) - 54003..54602 (-) 600 WP_022549504.1 XTP/dITP diphosphatase -
  VIBNI_RS00290 (VIBNI_A0051) - 54669..55100 (-) 432 WP_004398316.1 DUF4426 domain-containing protein -
  VIBNI_RS00295 (VIBNI_A0052) yggU 55124..55414 (-) 291 WP_022549505.1 DUF167 family protein YggU -
  VIBNI_RS00300 (VIBNI_A0053) - 55414..55971 (-) 558 WP_022549506.1 YggT family protein -
  VIBNI_RS00305 (VIBNI_A0054) proC 56001..56819 (-) 819 WP_022549507.1 pyrroline-5-carboxylate reductase -
  VIBNI_RS00310 (VIBNI_A0055) - 56881..57591 (-) 711 WP_004398312.1 YggS family pyridoxal phosphate-dependent enzyme -
  VIBNI_RS00315 (VIBNI_A0056) - 57615..58651 (+) 1037 Protein_49 type IV pilus twitching motility protein PilT -
  VIBNI_RS00320 (VIBNI_A0058) pilU 58660..59766 (+) 1107 WP_004398310.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VIBNI_RS00325 (VIBNI_A0059) ruvX 59850..60275 (-) 426 WP_022549508.1 Holliday junction resolvase RuvX -
  VIBNI_RS00330 (VIBNI_A0060) - 60328..60891 (-) 564 WP_004409298.1 YqgE/AlgH family protein -
  VIBNI_RS00335 (VIBNI_A0061) gshB 60918..61865 (-) 948 WP_022549509.1 glutathione synthase -
  VIBNI_RS00340 (VIBNI_A0062) rsmE 61880..62611 (-) 732 WP_004409303.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VIBNI_RS00345 (VIBNI_A0063) - 62717..63418 (-) 702 WP_022549510.1 endonuclease -
  VIBNI_RS00350 (VIBNI_A0064) - 63506..64006 (-) 501 WP_022549511.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41280.30 Da        Isoelectric Point: 5.6292

>NTDB_id=62556 VIBNI_RS00320 WP_004398310.1 58660..59766(+) (pilU) [Vibrio nigripulchritudo]
MELNQVLSEMVSLKASDIYITVGAPVLLRVHGELQPLGEPLTEQSAFHLLDTMMDEERRAEFRKERESNFAIVRDVGRFR
VSAFFQRELPGAVLRRIETQIPTFEELKLPDVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGFRNQNRTGHILTVEDPI
EFVHEHNQCIITQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIAQQLIRDKSGQGRHGVFEILLNTPRVSDLIRRGELHELKATMAKSNEAGMKTFDQCLY
ELLSADKITEQDAMHSADSANDLRLMMKTKQGGDFSGGALDGVKIDMN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=62556 VIBNI_RS00320 WP_004398310.1 58660..59766(+) (pilU) [Vibrio nigripulchritudo]
ATGGAACTGAATCAAGTTTTATCAGAAATGGTGTCTCTGAAGGCATCAGACATCTACATCACAGTGGGTGCGCCAGTCCT
GTTGCGTGTTCACGGAGAACTGCAACCATTGGGTGAACCACTGACCGAGCAGTCTGCCTTTCATCTGCTCGATACAATGA
TGGATGAAGAGCGTCGCGCGGAGTTTCGTAAAGAAAGAGAGTCCAACTTTGCTATCGTTCGAGATGTTGGACGTTTTCGT
GTGAGTGCGTTCTTCCAGCGTGAATTGCCCGGTGCGGTACTGCGCCGAATAGAGACGCAGATTCCCACCTTCGAAGAGCT
CAAATTACCGGATGTTCTGCAGGATCTTGCCATCGCTAAGCGAGGCTTAGTGCTGGTGGTTGGTGCAACTGGCTCAGGTA
AGTCCACCACCATGGCCGCGATGACGGGCTTTCGAAATCAGAATCGCACAGGTCATATCCTGACAGTGGAAGACCCGATT
GAATTTGTTCACGAGCACAATCAGTGCATCATCACCCAACGTGAAGTGGGTTTGGATACCGAGAGCTATGAGGTCGCACT
GAAGAATTCTCTTCGACAGGCGCCAGACATGATTCTGATTGGTGAAATTCGCTCCCGTGAAACTATGGAATATGCCATGA
CCTTTGCGGAAACCGGTCACCTCTGCATGGCAACACTGCACGCTAACAACGCAAACCAGGCATTGGAGCGTATTCTTCAC
CTGGTGCCTAAAGAGCAGAAAGAGCAGTTTCTGTTTGATTTATCCATGAACTTGCGCGGTGTGATAGCGCAGCAACTGAT
TCGCGATAAGAGCGGGCAAGGGCGGCATGGGGTATTCGAAATCCTTCTGAATACCCCAAGGGTGTCCGACCTGATTCGTC
GTGGTGAGCTGCACGAGCTGAAAGCAACGATGGCGAAATCCAACGAGGCGGGAATGAAAACCTTCGATCAGTGTTTGTAT
GAGCTTCTGTCGGCAGACAAGATCACCGAGCAGGATGCGATGCACAGCGCTGACTCTGCGAATGATTTGAGGTTGATGAT
GAAAACCAAGCAAGGCGGAGATTTCTCTGGCGGCGCGCTTGATGGTGTGAAAATAGACATGAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

80.381

99.728

0.802
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

53.846

95.38

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

92.12

0.399
  pilT Acinetobacter nosocomialis M2

41.471

92.391

0.383
  pilT Acinetobacter baylyi ADP1

41.349

92.663

0.383
  pilT Acinetobacter baumannii D1279779

41.176

92.391

0.38
  pilT Acinetobacter baumannii strain A118

41.176

92.391

0.38
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Legionella pneumophila strain Lp02

40.118

92.12

0.37
  pilT Legionella pneumophila strain ERS1305867

40.118

92.12

0.37
  pilT Pseudomonas aeruginosa PAK

39.823

92.12

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361

Multiple sequence alignment