Detailed information NTG0621124

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LIS66_RS12345
• Genome accession: NZ_CP085885
• Coordinates: 2823049..2824158 (+)
• Length: 369 a.a.
• NCBI ID: WP_083352915.1
• Uniprot ID: -
• Organism: Pseudomonas sp. HN2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2818049..2829158

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LIS66_RS12330 (LIS66_12330)	-	2819035..2819958 (+)	924	WP_064361768.1	sugar kinase	-
LIS66_RS12335 (LIS66_12335)	-	2820074..2820349 (-)	276	WP_007912322.1	peptidylprolyl isomerase	-
LIS66_RS12340 (LIS66_12340)	-	2820402..2822939 (-)	2538	WP_227974401.1	PAS domain-containing protein	-
LIS66_RS12345 (LIS66_12345)	pilU	2823049..2824158 (+)	1110	WP_083352915.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LIS66_RS12350 (LIS66_12350)	-	2824287..2827928 (+)	3642	WP_227974402.1	hypothetical protein	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41033.86 Da
• Isoelectric Point: 6.7312

>NTDB_id=621124 LIS66_RS12345 WP_083352915.1 2823049..2824158(+) (pilU) [Pseudomonas sp. HN2]
MEIDALLSTLSQKNGSDLFLSTGAPPSARVDGVLTALNDQPFKPGETAAIATSLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKLDIPQFEDLKLPPVLLETVMLKRGLILFVGATSSGKSTSLAALIDHRNRHSAGHIVTIED
PIEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVLSTLHATNANQALDRI
INMFPEERREQLLQTLGNNLKACVSQRLVRTVDGQRRAAVEVMLGNPTIEDHIRRNELGELKGIMEKSIDLGMQTFDGAL
YNLVLEGAITMEEAFKNADSINNIRLRLKLSGESIDAQGRHSGGWSLLE

Nucleotide

• Length: 1110 bp

>NTDB_id=621124 LIS66_RS12345 WP_083352915.1 2823049..2824158(+) (pilU) [Pseudomonas sp. HN2]
ATGGAAATCGATGCACTGTTATCAACATTGTCGCAAAAAAACGGTTCGGATCTGTTCCTCTCCACCGGCGCACCGCCCAG
CGCGCGCGTTGACGGTGTGTTGACGGCATTGAACGATCAGCCATTCAAACCCGGCGAAACCGCGGCAATCGCCACCTCCC
TGATGGACGCTGAGCAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATCTCCCGAACGGGCGTCGGGCGG
TTCCGGGTGAACATTTTCAAGCAGCGCAACGACGTCTCGATCGTCATCCGCAACGTCAAACTCGACATTCCGCAATTCGA
AGACTTGAAGCTGCCGCCAGTGTTGCTCGAGACGGTGATGCTCAAACGAGGCCTGATTCTCTTCGTCGGCGCCACCAGTT
CAGGCAAGTCGACCTCGCTGGCCGCCCTGATCGACCACCGCAACCGCCACAGTGCCGGGCACATCGTGACCATCGAGGAC
CCGATCGAATATATCCATCGGCACAAGCGTTCGATCATCAACCAGCGTGAAGTCGGCGTCGACACGCGCAGCTTTCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTCGTATTGATCGGCGAAATCCGTGATCGCGAAACCATGGAACATG
CATTGTCATTCGCCGATACCGGGCATCTGGTGCTGTCGACGTTGCACGCGACCAATGCCAATCAGGCACTCGATCGAATC
ATCAATATGTTTCCCGAGGAGCGGCGCGAGCAGCTTTTGCAAACCCTGGGCAACAACCTGAAGGCTTGTGTATCGCAAAG
ACTTGTACGCACGGTTGACGGGCAGAGAAGAGCAGCGGTGGAAGTCATGCTGGGGAATCCGACTATTGAAGATCACATTC
GGAGAAATGAGTTGGGTGAGTTGAAAGGCATAATGGAAAAATCCATCGATTTGGGTATGCAGACCTTTGATGGTGCTCTT
TACAACCTGGTTCTGGAGGGGGCGATCACTATGGAAGAAGCATTCAAGAATGCAGACTCCATAAATAACATCAGGCTGCG
ATTAAAACTTTCCGGCGAATCAATTGATGCGCAGGGGCGCCATTCTGGCGGCTGGAGTTTGTTGGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (57-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.62	96.206	0.545
pilU	Acinetobacter baylyi ADP1	51.685	96.477	0.499
pilU	Vibrio cholerae strain A1552	51.143	94.851	0.485
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.413	91.87	0.371
pilT	Pseudomonas aeruginosa PAK	39.535	93.225	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.535	93.225	0.369
pilT	Acinetobacter baumannii strain A118	40.299	90.786	0.366
pilT	Acinetobacter baumannii D1279779	40.299	90.786	0.366
pilT	Acinetobacter nosocomialis M2	40.299	90.786	0.366
pilT	Acinetobacter baylyi ADP1	39.412	92.141	0.363
pilT	Legionella pneumophila strain Lp02	39.645	91.599	0.363
pilT	Legionella pneumophila strain ERS1305867	39.645	91.599	0.363
pilT	Neisseria meningitidis 8013	39.528	91.87	0.363
pilT	Neisseria gonorrhoeae MS11	39.528	91.87	0.363