Detailed information NTG0616020

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: LG334_RS00525
• Genome accession: NZ_CP085224
• Coordinates: 121252..122511 (-)
• Length: 419 a.a.
• NCBI ID: WP_404382263.1
• Uniprot ID: -
• Organism: Caenispirillum salinarum strain ADH2-936
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 116252..127511

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG334_RS00505 (LG334_00510)	-	117701..118381 (-)	681	WP_404382255.1	type II secretion system protein	-
LG334_RS00510 (LG334_00515)	-	118482..119165 (-)	684	WP_404382257.1	type II secretion system protein	-
LG334_RS00515 (LG334_00520)	-	119423..120106 (-)	684	WP_404382259.1	hypothetical protein	-
LG334_RS00520 (LG334_00525)	pilU	120121..121236 (-)	1116	WP_404382261.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LG334_RS00525 (LG334_00530)	pilT	121252..122511 (-)	1260	WP_404382263.1	type IV pilus twitching motility protein PilT	Machinery gene
LG334_RS00530 (LG334_00535)	-	122591..123961 (-)	1371	WP_404382265.1	transglycosylase SLT domain-containing protein	-
LG334_RS00535 (LG334_00540)	-	124051..124944 (-)	894	WP_404382267.1	HAD-IA family hydrolase	-
LG334_RS00540 (LG334_00545)	-	125058..125360 (-)	303	WP_404382269.1	HigA family addiction module antitoxin	-
LG334_RS00545 (LG334_00550)	-	125421..125840 (-)	420	WP_404382271.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
LG334_RS00550 (LG334_00555)	-	125867..126844 (-)	978	WP_404382273.1	NADPH:quinone oxidoreductase family protein	-

Sequence

Protein

• Length: 419 a.a.
• Molecular weight: 45315.45 Da
• Isoelectric Point: 6.7328

>NTDB_id=616020 LG334_RS00525 WP_404382263.1 121252..122511(-) (pilT) [Caenispirillum salinarum strain ADH2-936]
MSEQELDITDLLAFTHQQGGSDLHLTSQREPMLRLSGELRPVKTGPLEPDTVKRMLTNIMSDELRAEYEREQECDFAISF
GRDSRFRVNAFTTITGPAAVFRTIPTKIPSLADLNAPDIFKNFADKEKGLVLVTGPTGSGKSTTLAAMIDHINETQNKHI
LTIEDPVEFVHDNKLSLINHREVGKHTKSFARALKSALREDPDVILVGEMRDHETISLALTAAETGHLVMGTLHTSSAAK
TIDRIIDVFPGDDKEMVRAMLAGSLQAVISQKLMKKIGGGRVAAHDILVGTDAVRNLIRENKVPQIFSMMQVGQRYGMQT
LKDSVQALVDQGVVDESEMKAFAKGDEEYQAQRAAQAAAGKGGGGSSARPEPARRPAAAQQQPAPPASEPPPPRAPEPAA
AEASSGGGRGKRGIRSFFG

Nucleotide

• Length: 1260 bp

>NTDB_id=616020 LG334_RS00525 WP_404382263.1 121252..122511(-) (pilT) [Caenispirillum salinarum strain ADH2-936]
ATGAGCGAGCAAGAGCTGGACATCACCGACCTCCTCGCCTTCACCCACCAGCAGGGCGGGTCGGACCTGCACCTGACCAG
CCAGCGCGAGCCCATGCTGCGCCTGTCGGGCGAGCTGCGGCCGGTCAAGACGGGGCCGCTGGAGCCCGACACGGTCAAGC
GCATGCTGACCAACATCATGTCCGATGAGCTGCGCGCCGAGTACGAGCGCGAGCAGGAATGCGACTTCGCCATCAGCTTC
GGCCGCGACTCGCGCTTCCGCGTCAATGCGTTCACCACCATCACCGGGCCGGCGGCGGTGTTCCGCACCATCCCGACCAA
GATCCCCAGCCTCGCGGATCTCAACGCGCCGGACATCTTCAAGAATTTCGCCGACAAGGAGAAGGGCCTGGTTCTGGTCA
CCGGCCCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACGAGACCCAGAACAAGCACATC
CTCACCATCGAAGACCCGGTGGAGTTCGTCCACGACAACAAGCTGTCGCTCATCAACCACCGCGAGGTGGGCAAGCACAC
CAAATCCTTCGCCCGCGCGCTGAAATCCGCCCTGCGCGAGGACCCCGACGTGATCCTGGTGGGCGAGATGCGCGACCACG
AGACCATCAGCCTGGCCCTGACGGCGGCCGAGACCGGCCACCTGGTCATGGGCACCCTGCACACCAGCAGCGCCGCCAAG
ACCATCGACCGCATCATCGACGTCTTCCCCGGCGACGATAAGGAAATGGTGCGCGCCATGCTGGCGGGTTCGCTTCAGGC
CGTCATCAGCCAGAAACTGATGAAGAAGATTGGCGGCGGGCGCGTCGCGGCGCACGACATCCTGGTCGGCACCGACGCCG
TGCGGAACCTGATCCGCGAGAACAAGGTGCCGCAGATCTTCTCCATGATGCAGGTGGGCCAGCGCTACGGCATGCAGACG
CTGAAGGACAGCGTGCAGGCGCTGGTCGATCAGGGCGTGGTGGACGAATCCGAGATGAAGGCCTTCGCCAAGGGCGACGA
GGAATACCAGGCCCAGCGCGCCGCTCAGGCCGCCGCCGGCAAGGGCGGCGGCGGTTCCTCCGCCCGGCCGGAGCCCGCCC
GGCGCCCGGCGGCGGCGCAGCAGCAGCCCGCCCCGCCCGCCTCCGAGCCCCCGCCGCCGCGCGCGCCCGAGCCGGCGGCC
GCCGAGGCCTCGTCCGGCGGCGGGCGCGGCAAGCGCGGCATCCGGTCCTTCTTCGGGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (17-276)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	60.417	80.191	0.484
pilT	Vibrio cholerae strain A1552	60.417	80.191	0.484
pilT	Neisseria gonorrhoeae MS11	61.515	78.759	0.484
pilT	Acinetobacter nosocomialis M2	58.721	82.1	0.482
pilT	Acinetobacter baumannii D1279779	58.721	82.1	0.482
pilT	Acinetobacter baumannii strain A118	58.721	82.1	0.482
pilT	Neisseria meningitidis 8013	61.212	78.759	0.482
pilT	Pseudomonas aeruginosa PAK	56.977	82.1	0.468
pilT	Pseudomonas stutzeri DSM 10701	56.395	82.1	0.463
pilT	Acinetobacter baylyi ADP1	56.395	82.1	0.463
pilT	Legionella pneumophila strain Lp02	57.402	78.998	0.453
pilT	Legionella pneumophila strain ERS1305867	57.402	78.998	0.453
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	47.215	89.976	0.425