Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LG334_RS00520 Genome accession   NZ_CP085224
Coordinates   120121..121236 (-) Length   371 a.a.
NCBI ID   WP_404382261.1    Uniprot ID   -
Organism   Caenispirillum salinarum strain ADH2-936     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 115121..126236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG334_RS00500 (LG334_00505) - 115635..117704 (-) 2070 WP_404382253.1 hypothetical protein -
  LG334_RS00505 (LG334_00510) - 117701..118381 (-) 681 WP_404382255.1 type II secretion system protein -
  LG334_RS00510 (LG334_00515) - 118482..119165 (-) 684 WP_404382257.1 type II secretion system protein -
  LG334_RS00515 (LG334_00520) - 119423..120106 (-) 684 WP_404382259.1 hypothetical protein -
  LG334_RS00520 (LG334_00525) pilU 120121..121236 (-) 1116 WP_404382261.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG334_RS00525 (LG334_00530) pilT 121252..122511 (-) 1260 WP_404382263.1 type IV pilus twitching motility protein PilT Machinery gene
  LG334_RS00530 (LG334_00535) - 122591..123961 (-) 1371 WP_404382265.1 transglycosylase SLT domain-containing protein -
  LG334_RS00535 (LG334_00540) - 124051..124944 (-) 894 WP_404382267.1 HAD-IA family hydrolase -
  LG334_RS00540 (LG334_00545) - 125058..125360 (-) 303 WP_404382269.1 HigA family addiction module antitoxin -
  LG334_RS00545 (LG334_00550) - 125421..125840 (-) 420 WP_404382271.1 secondary thiamine-phosphate synthase enzyme YjbQ -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40771.91 Da        Isoelectric Point: 5.2711

>NTDB_id=616019 LG334_RS00520 WP_404382261.1 120121..121236(-) (pilU) [Caenispirillum salinarum strain ADH2-936]
MMLDYLHAWLTDLSGPKGSDLYITVGAPPILRGDDFTPLIDDPLQAGHVQAIVDEFLTDEQRAVFEREREFNMSYMMEGV
GRFRVNIFMQRGLPGFVIRKITTEIPTLADLHLPDQLYGLAEAKRGLVIMVGGTGSGKSTTLAAMIGHRNHSAPGHIITI
EDPVEYVHEHARCIVTQREVGVDTASFESALKNALRQKPDLILLGEIRDATTMEYAINIAETGHLCLATLHANNANQALD
RVLSFFPQEMHRQVLLNLSMNLKALLSQRLVRTTQGGRRAALEILLNEGLVTDLIRKGEVKDLKKVMAENRALGMLTFDQ
SLLDLVGAGVIDEDTAIAEADTPADLKIALREQKMGGEAGLTGVDTSRLSL

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=616019 LG334_RS00520 WP_404382261.1 120121..121236(-) (pilU) [Caenispirillum salinarum strain ADH2-936]
ATGATGCTCGACTACCTGCACGCCTGGCTGACGGACCTGTCCGGCCCCAAGGGATCGGACCTCTACATCACCGTCGGCGC
CCCGCCCATCCTGCGCGGCGACGACTTCACGCCGCTGATCGACGATCCGCTCCAGGCCGGGCACGTCCAGGCCATCGTCG
ACGAATTCCTGACCGACGAGCAGCGCGCCGTGTTCGAGCGCGAGCGCGAGTTCAACATGAGCTACATGATGGAGGGCGTG
GGCCGCTTCCGCGTCAACATCTTCATGCAGCGCGGCCTGCCGGGCTTCGTCATCCGCAAGATCACCACGGAAATCCCCAC
GCTCGCCGACCTGCACCTGCCCGACCAGCTGTACGGGCTGGCGGAGGCCAAGCGCGGGCTGGTCATCATGGTGGGCGGCA
CGGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGATCGGCCACCGCAACCACAGCGCGCCGGGGCACATCATCACCATC
GAGGACCCGGTGGAATACGTGCACGAGCACGCCCGCTGCATCGTCACCCAGCGCGAGGTGGGGGTGGACACGGCCAGCTT
CGAATCGGCGCTCAAGAACGCGCTGCGCCAGAAGCCCGACCTGATCCTGCTGGGCGAGATCCGCGACGCCACCACCATGG
AATACGCCATCAACATCGCGGAAACGGGCCACCTGTGTCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAC
CGGGTGTTGAGCTTCTTCCCGCAGGAAATGCACCGCCAGGTGCTGCTGAACCTGTCCATGAACCTCAAGGCGCTGCTGTC
GCAACGGCTGGTGCGCACGACGCAGGGCGGGCGGCGCGCGGCGCTGGAAATCCTGCTGAACGAGGGGCTGGTGACCGACC
TCATCCGCAAGGGCGAGGTCAAGGACCTGAAGAAGGTCATGGCCGAGAACCGGGCGCTGGGGATGCTGACCTTCGACCAG
TCGCTGCTGGACCTGGTGGGCGCCGGCGTCATCGACGAGGACACCGCCATCGCCGAGGCCGACACCCCCGCCGACCTCAA
GATCGCCCTGCGCGAGCAGAAGATGGGCGGCGAGGCCGGGCTGACGGGCGTGGACACCTCGCGCCTGTCGCTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

52.857

94.34

0.499
  pilU Pseudomonas stutzeri DSM 10701

53.061

92.453

0.491
  pilU Acinetobacter baylyi ADP1

49.03

97.305

0.477
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.84

90.836

0.38
  pilT Legionella pneumophila strain ERS1305867

42.679

86.523

0.369
  pilT Legionella pneumophila strain Lp02

42.679

86.523

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.212

88.949

0.367
  pilT Pseudomonas aeruginosa PAK

40.909

88.949

0.364
  pilT Acinetobacter baumannii D1279779

41.284

88.14

0.364
  pilT Acinetobacter baumannii strain A118

41.284

88.14

0.364
  pilT Acinetobacter nosocomialis M2

41.284

88.14

0.364