Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N175_RS13100 Genome accession   NC_022223
Coordinates   2879821..2880927 (-) Length   368 a.a.
NCBI ID   WP_020977822.1    Uniprot ID   -
Organism   Vibrio anguillarum M3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2874821..2885927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N175_RS13070 (N175_13535) - 2875467..2875967 (+) 501 WP_013857812.1 SprT family zinc-dependent metalloprotease -
  N175_RS13075 (N175_13540) endA 2876099..2876806 (+) 708 WP_013857813.1 deoxyribonuclease I -
  N175_RS13080 (N175_13545) rsmE 2876915..2877646 (+) 732 WP_013857814.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N175_RS13085 (N175_13550) gshB 2877660..2878613 (+) 954 WP_040122940.1 glutathione synthase -
  N175_RS13090 (N175_13555) - 2878660..2879223 (+) 564 WP_013857816.1 YqgE/AlgH family protein -
  N175_RS13095 (N175_13560) ruvX 2879309..2879737 (+) 429 WP_013857817.1 Holliday junction resolvase RuvX -
  N175_RS13100 (N175_13565) pilU 2879821..2880927 (-) 1107 WP_020977822.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N175_RS13105 (N175_13570) pilT 2880938..2881975 (-) 1038 WP_013857819.1 type IV pilus twitching motility protein PilT Machinery gene
  N175_RS13110 (N175_13575) - 2882001..2882702 (+) 702 WP_013857820.1 YggS family pyridoxal phosphate-dependent enzyme -
  N175_RS13115 (N175_13580) proC 2882764..2883582 (+) 819 WP_040122939.1 pyrroline-5-carboxylate reductase -
  N175_RS13120 (N175_13585) - 2883645..2884202 (+) 558 WP_010320643.1 YggT family protein -
  N175_RS13125 (N175_13590) yggU 2884202..2884492 (+) 291 WP_040122938.1 DUF167 family protein YggU -
  N175_RS13130 (N175_13595) - 2884535..2884966 (+) 432 WP_013857823.1 DUF4426 domain-containing protein -
  N175_RS13135 (N175_13600) - 2884979..2885578 (+) 600 WP_013857824.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41381.43 Da        Isoelectric Point: 6.5071

>NTDB_id=61384 N175_RS13100 WP_020977822.1 2879821..2880927(-) (pilU) [Vibrio anguillarum M3]
MELNQCLEGMLTQKASDLYLTVGAPVLFRVDGELRAHGEKLSQTQVMALLDAIMDEDRRREFQRTREANFAVVREIGRFR
VSAFFQRELPGAVIRRIETHIPTFEELKLPGVLQDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNSHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEILLNSPRISDLIRRGDLHELKATMGKSKEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGEGISSGTLDNVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=61384 N175_RS13100 WP_020977822.1 2879821..2880927(-) (pilU) [Vibrio anguillarum M3]
ATGGAACTGAACCAATGTCTTGAGGGTATGTTGACTCAAAAAGCTTCCGACTTGTACCTCACCGTAGGAGCGCCAGTGTT
ATTTCGCGTCGATGGAGAGTTAAGAGCTCATGGCGAAAAGTTATCCCAAACGCAGGTGATGGCGTTGTTAGATGCCATTA
TGGATGAGGATCGCCGCCGTGAATTTCAACGCACACGTGAAGCCAATTTTGCAGTGGTGAGAGAGATTGGCCGTTTTCGT
GTCAGTGCTTTTTTTCAGCGTGAGCTGCCCGGTGCGGTGATCCGCCGTATTGAAACTCATATCCCCACTTTTGAAGAATT
AAAGTTACCGGGCGTGTTGCAAGATCTCTCGATTGCAAAGCGTGGGCTGGTGCTGGTGGTAGGCGCGACGGGGTCGGGCA
AATCGACCACCATGGCGGCCATGACGGGTTATCGTAACTCGCACCGAACAGGGCATATTTTAACGGTGGAAGATCCGATT
GAGTTTGTGCATGAGCATCAGCGTTGCATTGTGACCCAGCGAGAAGTGGGGTTGGATACCGAGAGTTACGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCTGATATGATTTTAATTGGTGAGATCCGTTCACGTGAAACGATGGAATATGCGATGA
CCTTTGCCGAGACAGGTCATTTATGTATGGCAACGTTACATGCTAACAATGCCAACCAAGCGCTTGAGCGAATTTTGCAT
TTGGTTCCTAAAGAGCAAAAAGAGCAGTTTTTGTTTGATCTGTCGATGAACCTCAAAGGCGTCGTGGGGCAGCAGTTGAT
CCGCGATAAAAATGGCAAGGGACGACATGGTGTGTTTGAGATTTTACTCAACAGCCCTCGAATTTCCGATTTGATCCGCC
GCGGTGATCTGCACGAATTGAAAGCGACGATGGGCAAATCAAAAGAAGTGGGGATGCAGACTTTTGACCAAGCACTGTAT
CAACTGGTCGTTGATGACAAAATCAGTGAACAAGATGCACTGCACAGCGCTGACTCGGCTAACGATTTGCGTTTAATGTT
AAAAACAAAGCGTGGCGAAGGGATCAGCAGTGGCACATTGGATAACGTGCAGATTGATATGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

87.228

100

0.872
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

53.846

95.38

0.514
  pilT Pseudomonas aeruginosa PAK

42.773

92.12

0.394
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.872

84.783

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.298

92.12

0.38
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372

Multiple sequence alignment