Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LA331_RS09035 Genome accession   NZ_CP083658
Coordinates   1816658..1817122 (+) Length   154 a.a.
NCBI ID   WP_005233603.1    Uniprot ID   N9MQ57
Organism   Acinetobacter variabilis strain FDAARGOS_1487     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1811658..1822122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA331_RS09015 (LA331_09015) - 1813221..1813604 (+) 384 WP_005233608.1 RidA family protein -
  LA331_RS09020 (LA331_09020) - 1813760..1813954 (+) 195 WP_005233606.1 bacterioferritin-associated ferredoxin -
  LA331_RS09025 (LA331_09025) bfr 1814181..1814645 (+) 465 WP_005233605.1 heteropolymeric bacterioferritin subunit Bfr -
  LA331_RS09030 (LA331_09030) - 1814701..1816338 (-) 1638 WP_005233604.1 PglL family O-oligosaccharyltransferase -
  LA331_RS09035 (LA331_09035) pilA 1816658..1817122 (+) 465 WP_005233603.1 pilin Machinery gene
  LA331_RS09045 (LA331_09045) - 1817434..1817994 (-) 561 WP_005233602.1 TPM domain-containing protein -
  LA331_RS09050 (LA331_09050) - 1817988..1819034 (-) 1047 WP_005233601.1 TPM domain-containing protein -
  LA331_RS09055 (LA331_09055) - 1819072..1819662 (-) 591 WP_005233599.1 LemA family protein -
  LA331_RS09060 (LA331_09060) - 1819851..1821272 (-) 1422 WP_005233597.1 FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15492.65 Da        Isoelectric Point: 8.4502

>NTDB_id=607180 LA331_RS09035 WP_005233603.1 1816658..1817122(+) (pilA) [Acinetobacter variabilis strain FDAARGOS_1487]
MNAQKGFTLIELMIVVAIIGILAAIAVPAYQDYTVRAKVSEGLSLAGAAKTTVAENAASAAPFAAGYTSPVTKNVSGISI
DETTGVITITYTAAAGGATNANTITLTPSYKGGTNGTTVTALAGTANASVVPTNAIEWTCAGTMPTKYRPSNCR

Nucleotide


Download         Length: 465 bp        

>NTDB_id=607180 LA331_RS09035 WP_005233603.1 1816658..1817122(+) (pilA) [Acinetobacter variabilis strain FDAARGOS_1487]
ATGAATGCTCAAAAAGGTTTTACCCTTATTGAATTAATGATTGTTGTCGCTATTATCGGTATTTTAGCTGCAATTGCTGT
ACCTGCATATCAAGACTACACAGTTCGTGCAAAAGTATCTGAAGGTTTAAGTTTAGCTGGTGCTGCAAAAACAACTGTAG
CTGAAAATGCAGCAAGCGCTGCCCCATTTGCCGCTGGTTATACATCACCAGTCACAAAAAATGTATCTGGTATTAGTATC
GATGAGACTACAGGTGTAATTACAATTACATATACTGCAGCTGCTGGTGGTGCTACAAATGCAAATACTATTACTTTAAC
CCCATCATATAAAGGTGGTACAAATGGTACTACTGTAACTGCATTAGCAGGCACTGCAAATGCTAGTGTAGTACCTACTA
ATGCAATTGAATGGACGTGTGCAGGAACTATGCCTACTAAATACCGCCCATCAAATTGCCGATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9MQ57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

49.711

100

0.558
  pilA2 Legionella pneumophila str. Paris

54.43

100

0.558
  pilA2 Legionella pneumophila strain ERS1305867

54.43

100

0.558
  comP Acinetobacter baylyi ADP1

49.045

100

0.5
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.563

100

0.481
  pilA/pilA1 Eikenella corrodens VA1

36.782

100

0.416
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.397

98.052

0.396
  pilA Acinetobacter baumannii strain A118

37.5

100

0.39
  pilA Pseudomonas aeruginosa PAK

37.58

100

0.383
  pilE Neisseria gonorrhoeae MS11

37.107

100

0.383
  pilA Vibrio cholerae C6706

36.478

100

0.377
  pilA Vibrio cholerae strain A1552

36.478

100

0.377
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.478

100

0.377
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38

97.403

0.37