Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   K3819_RS24745 Genome accession   NZ_CP081200
Coordinates   5462797..5463219 (-) Length   140 a.a.
NCBI ID   WP_221398117.1    Uniprot ID   -
Organism   Pseudomonas sp. 3-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5457797..5468219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3819_RS24730 (K3819_24730) - 5458726..5461677 (+) 2952 WP_221398116.1 DUF748 domain-containing protein -
  K3819_RS24735 (K3819_24735) - 5461677..5461973 (+) 297 WP_085648751.1 DUF2845 domain-containing protein -
  K3819_RS24740 (K3819_24740) - 5462143..5462499 (-) 357 WP_210710590.1 BON domain-containing protein -
  K3819_RS24745 (K3819_24745) pilA/pilAI 5462797..5463219 (-) 423 WP_221398117.1 pilin Machinery gene
  K3819_RS24750 (K3819_24750) pilB 5463446..5465146 (+) 1701 WP_221398118.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K3819_RS24755 (K3819_24755) pilC 5465149..5466366 (+) 1218 WP_221398119.1 type II secretion system F family protein Machinery gene
  K3819_RS24760 (K3819_24760) pilD 5466368..5467240 (+) 873 WP_064599491.1 A24 family peptidase Machinery gene
  K3819_RS24765 (K3819_24765) coaE 5467237..5467860 (+) 624 WP_169431959.1 dephospho-CoA kinase -
  K3819_RS24770 (K3819_24770) yacG 5467857..5468057 (+) 201 WP_039771664.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14045.14 Da        Isoelectric Point: 8.1079

>NTDB_id=595604 K3819_RS24745 WP_221398117.1 5462797..5463219(-) (pilA/pilAI) [Pseudomonas sp. 3-2]
MNKQQGFTLIELLIVVAIIGILATVALPQYSKYQARSKVTAGLAEISAMKVPFEDIINQGTNPTLAAVKGDAATSTSNCA
LSASGTAADGAGSIVCTLANAPAPVVGKTITLTRTAANGWSCATTVAPDYSPKGCVASGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=595604 K3819_RS24745 WP_221398117.1 5462797..5463219(-) (pilA/pilAI) [Pseudomonas sp. 3-2]
ATGAATAAACAACAGGGTTTCACTCTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGTATTCTCGCGACTGTGGCGCT
GCCGCAGTACTCCAAATATCAGGCGCGGTCGAAGGTGACGGCGGGGTTGGCCGAGATCTCGGCGATGAAAGTGCCGTTCG
AAGACATCATCAATCAAGGAACGAACCCCACGCTGGCCGCCGTCAAAGGCGATGCGGCTACTTCCACCTCCAACTGTGCG
TTGAGCGCTTCGGGGACGGCTGCAGATGGAGCAGGCTCCATCGTGTGTACGCTGGCCAATGCGCCGGCACCCGTGGTGGG
CAAAACCATTACGCTCACGCGTACGGCTGCCAACGGCTGGTCATGTGCAACGACAGTCGCGCCGGACTATTCACCTAAAG
GCTGCGTTGCGAGCGGTACCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50

97.143

0.486
  pilA Acinetobacter baumannii strain A118

46.763

99.286

0.464
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.667

100

0.464
  pilA Vibrio cholerae strain A1552

41.667

100

0.464
  pilA Vibrio cholerae C6706

41.667

100

0.464
  comP Acinetobacter baylyi ADP1

42.105

100

0.457
  pilA Pseudomonas aeruginosa PAK

41.216

100

0.436
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.382

97.143

0.421
  pilA2 Legionella pneumophila str. Paris

41.429

100

0.414
  pilA2 Legionella pneumophila strain ERS1305867

40.714

100

0.407
  pilA Vibrio parahaemolyticus RIMD 2210633

41.791

95.714

0.4