Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K3819_RS24750 Genome accession   NZ_CP081200
Coordinates   5463446..5465146 (+) Length   566 a.a.
NCBI ID   WP_221398118.1    Uniprot ID   -
Organism   Pseudomonas sp. 3-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5458446..5470146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3819_RS24730 (K3819_24730) - 5458726..5461677 (+) 2952 WP_221398116.1 DUF748 domain-containing protein -
  K3819_RS24735 (K3819_24735) - 5461677..5461973 (+) 297 WP_085648751.1 DUF2845 domain-containing protein -
  K3819_RS24740 (K3819_24740) - 5462143..5462499 (-) 357 WP_210710590.1 BON domain-containing protein -
  K3819_RS24745 (K3819_24745) pilA/pilAI 5462797..5463219 (-) 423 WP_221398117.1 pilin Machinery gene
  K3819_RS24750 (K3819_24750) pilB 5463446..5465146 (+) 1701 WP_221398118.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K3819_RS24755 (K3819_24755) pilC 5465149..5466366 (+) 1218 WP_221398119.1 type II secretion system F family protein Machinery gene
  K3819_RS24760 (K3819_24760) pilD 5466368..5467240 (+) 873 WP_064599491.1 A24 family peptidase Machinery gene
  K3819_RS24765 (K3819_24765) coaE 5467237..5467860 (+) 624 WP_169431959.1 dephospho-CoA kinase -
  K3819_RS24770 (K3819_24770) yacG 5467857..5468057 (+) 201 WP_039771664.1 DNA gyrase inhibitor YacG -
  K3819_RS24775 (K3819_24775) - 5468064..5468279 (-) 216 WP_007959584.1 hypothetical protein -
  K3819_RS24780 (K3819_24780) - 5468345..5469034 (-) 690 WP_085612628.1 energy-coupling factor ABC transporter permease -
  K3819_RS24785 (K3819_24785) - 5469168..5469794 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62828.83 Da        Isoelectric Point: 5.9468

>NTDB_id=595605 K3819_RS24750 WP_221398118.1 5463446..5465146(+) (pilB) [Pseudomonas sp. 3-2]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHATGLEEMADVDLDG
LDIESVDDSRQDTLGGIDAEDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPAGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDTQMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=595605 K3819_RS24750 WP_221398118.1 5463446..5465146(+) (pilB) [Pseudomonas sp. 3-2]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAACTGGTCCAGGCCGAATTGCTCACGGAAAAGAGTGCCCAGCAGGC
CTGGCAACAGGCGCAGCGCAATCGCCTGTCGCTGGTCAGTTACCTGGTGCAGAACAAACTGGTGAAGAGCTGGCAGGTTG
CGGAAATTGCCTCGGAGCATTTCGGCATGGCATTTCTCGACCTCAACTGCCTCGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCACCATGCCCTGCCCCTCTGGCGGCGTGGCAACAAACTGTTCGTGGGGATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGACGCCATCGAAAAATTCTTCGACACCCACGCCACCGGACTGGAGGAAATGGCCGATGTCGACCTTGACGGG
CTGGACATCGAATCGGTCGACGACAGCAGGCAGGACACCCTCGGCGGAATCGACGCCGAAGATGCCCCGGTAGTGCGCTT
CGTGCACAAGATGCTGCTGGACGCGATCAAGAGCGGCTCATCCGACCTGCACTTCGAGCCTTACGAAAAGAACTACCGGG
TGCGGGTGCGCACCGACGGCATTCTGCGGGAAGTGGCCAAGCCGCCGATTCAACTGGCCGGGCGCATCGCTGCACGCTTG
AAGGTCATGGCCAGCCTCGACATTTCGGAGCGACGCAAACCCCAGGACGGGCGGATCAAGATGCGTCTGTCGAAAAGCAA
GTCGATCGATTTCCGGGTCAACACCCTGCCCACCCTCTGGGGGGAGAAAGTGGTGATTCGGATCCTCGACCCGTCCAGCG
CCCAGATCGGCATCGATGCCCTCGGCTACGAGCCGGAGCAGAAAGACCTGTACATGGCCGCGCTCAAACAGCCGCAAGGC
ATGATTCTGGTGACCGGCCCCACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAACATCCTCAATACCGTCGA
CATCAATATCTCCACGGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCCGTCAGG
GACTGGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGTGACCTC
GAAACCGCCGAAATCGCGATCAAGGCCGCCCAGACCGGTCACCTCGTGCTGTCCACCCTGCACACCAACAGCGCAGCAGA
GACCCTCACCCGCCTGCACAACATGGGCATCCCCGGCTTCAACATCGCGACCTCGGTGAGCCTGATCATTGCCCAGCGTC
TGGCGCGAAAACTCTGCAGCCACTGCAAGAAACCCATCGAGATCCCACGCGAGACCCTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTATGAGCCCGCCGGTTGCGATCACTGCAACGGCGGGTACAAGGGTCGCGTGGGGAT
TTATGAAGTGGTAAAGAACACCCCCGATCTGCAACGGCTGATCATGGCCGAGGGCAACTCACTGGAAATCGATACCCAGA
TGCGCCGGGACGGCTTCAATGACCTGCGTACCTCGGGCCTGATCAAGGCCATGCAAGGCATTACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.848

100

0.58
  pilB Acinetobacter baumannii D1279779

57.496

100

0.576
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.558
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

49.383

100

0.495
  pilB Vibrio campbellii strain DS40M4

48.438

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.023

99.47

0.488
  pilF Thermus thermophilus HB27

36.559

98.587

0.36