Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   K0I63_RS17740 Genome accession   NZ_CP080420
Coordinates   4102096..4102542 (+) Length   148 a.a.
NCBI ID   WP_220081890.1    Uniprot ID   -
Organism   Shewanella rhizosphaerae strain FJAT-53764     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4097096..4107542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0I63_RS17720 (K0I63_17720) pdhR 4097400..4098152 (-) 753 WP_011867228.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  K0I63_RS17725 (K0I63_17725) ampE 4098391..4099242 (-) 852 WP_220081888.1 beta-lactamase regulator AmpE -
  K0I63_RS17730 (K0I63_17730) ampD 4099299..4099844 (-) 546 WP_220081889.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K0I63_RS17735 (K0I63_17735) nadC 4100291..4101172 (+) 882 WP_011867231.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K0I63_RS17740 (K0I63_17740) pilA/pilAI 4102096..4102542 (+) 447 WP_220081890.1 pilin Machinery gene
  K0I63_RS17745 (K0I63_17745) pilB 4102630..4104339 (+) 1710 WP_220081891.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K0I63_RS17750 (K0I63_17750) pilC 4104427..4105692 (+) 1266 WP_220081892.1 type II secretion system F family protein Machinery gene
  K0I63_RS17755 (K0I63_17755) pilD 4105732..4106643 (+) 912 WP_220081893.1 A24 family peptidase Machinery gene
  K0I63_RS17760 (K0I63_17760) coaE 4106664..4107269 (+) 606 WP_220081894.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15411.74 Da        Isoelectric Point: 8.0922

>NTDB_id=592815 K0I63_RS17740 WP_220081890.1 4102096..4102542(+) (pilA/pilAI) [Shewanella rhizosphaerae strain FJAT-53764]
MKGFKKTNAKGFTLIELMIVVAIIGILAAIALPAYQDYTIKSQVGGGLSEVSGLKNAFEVAVLDDIDPSLVTTNAGYVGQ
QATTGTYCDLALTARTAMECTLKGGNATYVNGKKLTLNRSADGVWTCNTDVDAKYRPKGCTAAAAPAP

Nucleotide


Download         Length: 447 bp        

>NTDB_id=592815 K0I63_RS17740 WP_220081890.1 4102096..4102542(+) (pilA/pilAI) [Shewanella rhizosphaerae strain FJAT-53764]
ATGAAAGGTTTCAAAAAGACTAACGCTAAGGGTTTCACCCTGATTGAATTAATGATCGTTGTTGCAATCATCGGTATTCT
CGCCGCAATTGCACTTCCTGCTTATCAAGATTACACAATTAAATCTCAAGTCGGTGGTGGCCTGTCAGAAGTTTCTGGGC
TCAAAAATGCTTTTGAAGTTGCCGTCTTGGACGATATCGACCCTTCTCTAGTGACAACAAACGCTGGTTATGTTGGTCAA
CAAGCTACAACCGGTACTTATTGCGATCTAGCCTTAACAGCTAGAACTGCCATGGAATGTACATTAAAAGGTGGTAACGC
CACTTATGTCAATGGTAAAAAGCTTACCTTAAATAGAAGTGCAGATGGTGTGTGGACATGTAATACAGATGTTGATGCTA
AATATCGTCCTAAAGGCTGTACTGCTGCGGCTGCTCCAGCTCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)

Pilin (bacterial filament) (Pilin)

(41-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

55.97

90.541

0.507
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.079

100

0.453
  pilA Vibrio cholerae strain A1552

44.079

100

0.453
  pilA Vibrio cholerae C6706

44.079

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

48.175

92.568

0.446
  pilA Acinetobacter baumannii strain A118

47.794

91.892

0.439
  pilA2 Legionella pneumophila str. Paris

45.185

91.216

0.412
  pilA2 Legionella pneumophila strain ERS1305867

44.444

91.216

0.405
  pilA Pseudomonas aeruginosa PAK

40.411

98.649

0.399
  comP Acinetobacter baylyi ADP1

37.748

100

0.385
  pilE Neisseria gonorrhoeae MS11

35

100

0.378