Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K0I63_RS17745 Genome accession   NZ_CP080420
Coordinates   4102630..4104339 (+) Length   569 a.a.
NCBI ID   WP_220081891.1    Uniprot ID   -
Organism   Shewanella rhizosphaerae strain FJAT-53764     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4097630..4109339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0I63_RS17725 (K0I63_17725) ampE 4098391..4099242 (-) 852 WP_220081888.1 beta-lactamase regulator AmpE -
  K0I63_RS17730 (K0I63_17730) ampD 4099299..4099844 (-) 546 WP_220081889.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K0I63_RS17735 (K0I63_17735) nadC 4100291..4101172 (+) 882 WP_011867231.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K0I63_RS17740 (K0I63_17740) pilA/pilAI 4102096..4102542 (+) 447 WP_220081890.1 pilin Machinery gene
  K0I63_RS17745 (K0I63_17745) pilB 4102630..4104339 (+) 1710 WP_220081891.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K0I63_RS17750 (K0I63_17750) pilC 4104427..4105692 (+) 1266 WP_220081892.1 type II secretion system F family protein Machinery gene
  K0I63_RS17755 (K0I63_17755) pilD 4105732..4106643 (+) 912 WP_220081893.1 A24 family peptidase Machinery gene
  K0I63_RS17760 (K0I63_17760) coaE 4106664..4107269 (+) 606 WP_220081894.1 dephospho-CoA kinase -
  K0I63_RS17765 (K0I63_17765) zapD 4107278..4108012 (+) 735 WP_220081895.1 cell division protein ZapD -
  K0I63_RS17770 (K0I63_17770) yacG 4108061..4108273 (+) 213 WP_041407493.1 DNA gyrase inhibitor YacG -
  K0I63_RS17775 (K0I63_17775) mutT 4108274..4108663 (+) 390 WP_220081896.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63093.16 Da        Isoelectric Point: 4.8926

>NTDB_id=592816 K0I63_RS17745 WP_220081891.1 4102630..4104339(+) (pilB) [Shewanella rhizosphaerae strain FJAT-53764]
MPTTGLHLGLSTLFIRKQMLSEEEVATAITKSRQSKLSLVSTIVSEKLLSAREIAELCYEEYGTPLLDLNEFDISSIPED
FLNKKLIEKHKCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEDDKLTLALEKVLEEDISALDLDGIDEDS
LSGIEITDTDKRQEEQQGDASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLAGRISA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRALYEEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCTECRQPEDVPEHELLNLGF
TQAQIDQGFTVYKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIANQAKEQGMRDLRESGLRKVIAGVTSI
TEINRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=592816 K0I63_RS17745 WP_220081891.1 4102630..4104339(+) (pilB) [Shewanella rhizosphaerae strain FJAT-53764]
ATGCCTACAACTGGACTTCATCTAGGGTTATCCACGCTATTTATTCGCAAACAAATGTTAAGCGAAGAAGAAGTTGCAAC
GGCAATAACGAAATCTCGCCAATCAAAATTATCTTTAGTATCAACGATAGTATCGGAAAAACTACTATCAGCCAGAGAAA
TAGCAGAACTCTGCTACGAAGAATATGGCACGCCGCTGTTAGACCTAAATGAATTCGACATAAGCAGCATTCCAGAAGAT
TTTCTCAACAAGAAACTGATAGAAAAACATAAATGTTTACCCTTATTTAAACGTGGTAATCGCCTTTATATCGGCACCTC
AGATCCGACCAATATTGCCGCCTTAGAAGATTTTCAATTTAGTGCAGGGCTCCATGCCGAAGCCATTCTGGTCGAAGATG
ACAAGTTAACCTTAGCGTTAGAAAAGGTTCTCGAAGAAGATATATCAGCGCTCGATCTAGATGGTATAGATGAAGACTCT
TTATCCGGCATTGAGATTACCGATACAGACAAGCGCCAGGAAGAACAGCAGGGTGATGCCAGTGATGACGCCCCTATCGT
CATCTATATCAATAAAATATTAACAGATGCCATACGTAAAGGCGCATCAGATCTGCACTTTGAGCCTTACGAGAAGCGTT
ACCGCATTCGCTTTCGTATCGATGGCATATTACACGAAGTCTCTGAGCCACCGGTTAACTTGGCTGGTCGTATCTCAGCT
CGTTTAAAAGTAATGTCTAAGCTCGATATCGCCGAACGCCGCGTGCCCCAGGATGGCCGTATCAAGATGAAGCTATCTCG
CACTAAGTCAATCGACTTTCGAGTCAGCACCTTGCCCACCATCTGGGGCGAAAAAATCGTGATGCGTATCTTGGACTCTT
CTTCGGCGCAGCTCGGCATCGAAAAGCTAGGTTATGAAGACGATCAAAGGGCACTATACGAGGAGATGCTTGCCAAACCT
CAAGGCATGATCTTGGTAACCGGCCCTACAGGCTCAGGTAAAACTGTATCTCTCTACACAGGCCTTAATATTCTCAACAC
CGAAGAGCGCAATATTTCAACCGCAGAAGACCCAGTTGAAATTAACTTAGAAGGCGTCAATCAGGTTCATATTAACCTAA
AAGCGGGCCTTACCTTCGCTTCTGCTCTGCGCTCATTTCTGCGTCAAGATCCAGATGTAGTCATGGTTGGTGAGATCCGT
GACCTGGAAACCGCTGAAATCGCCATTAAGGCTGCGCAAACCGGCCACTTGGTGCTATCAACCCTACATACCAACTCGGC
TGCGGAAACACTCACTCGTCTCATCAATATGGGCGTCCCCGGATACAACATCGCCAGCTCAGTTAATCTAATTATTGCAC
AGCGTCTGGCGAGAAGGCTCTGCACCGAATGCCGTCAACCAGAAGACGTGCCAGAGCACGAACTACTCAATTTAGGCTTT
ACCCAAGCACAAATAGATCAAGGATTTACTGTCTACAAGCCCGTTGGCTGCGATCTCTGTTCAGGCGGCTATAAAGGTCG
CGTAGGTATTTATGAGGTAATGAAAATGTCAGATGAGATTGCTCGTACTATCATGGAAGGAGGCAACTCGTTACAAATAG
CAAACCAGGCCAAAGAGCAAGGCATGCGAGATCTACGGGAATCTGGACTACGCAAGGTCATTGCCGGTGTGACGAGCATT
ACCGAAATAAACCGCGTCACCAGTTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.467

98.594

0.576
  pilB Acinetobacter baumannii D1279779

57.576

98.594

0.568
  pilB Legionella pneumophila strain ERS1305867

55.654

99.473

0.554
  pilB Vibrio cholerae strain A1552

51.246

98.77

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

50.439

100

0.504
  pilB Vibrio campbellii strain DS40M4

50.799

98.946

0.503
  pilF Neisseria gonorrhoeae MS11

46.643

99.473

0.464
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.138

97.891

0.383
  pilF Thermus thermophilus HB27

37.699

99.297

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

39.619

92.267

0.366