Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L483_RS30055 Genome accession   NC_021491
Coordinates   6493316..6494311 (-) Length   331 a.a.
NCBI ID   WP_016489636.1    Uniprot ID   A0A1L7NL30
Organism   Pseudomonas putida H8234     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6488316..6499311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L483_RS30035 (L483_30590) argS 6489550..6491286 (+) 1737 WP_016489632.1 arginine--tRNA ligase -
  L483_RS30040 (L483_30595) - 6491288..6491986 (+) 699 WP_016489633.1 SPOR domain-containing protein -
  L483_RS30045 (L483_30600) - 6492053..6492469 (-) 417 WP_016489634.1 NINE protein -
  L483_RS30050 (L483_30605) - 6492636..6493262 (+) 627 WP_016489635.1 C40 family peptidase -
  L483_RS30055 (L483_30610) pilT 6493316..6494311 (-) 996 WP_016489636.1 type IV pilus twitching motility protein PilT Machinery gene
  L483_RS30060 (L483_30615) - 6494377..6495063 (+) 687 WP_016489637.1 YggS family pyridoxal phosphate-dependent enzyme -
  L483_RS30065 (L483_30620) proC 6495102..6495920 (+) 819 WP_016489638.1 pyrroline-5-carboxylate reductase -
  L483_RS30070 (L483_30625) - 6495931..6496521 (+) 591 WP_016489639.1 YggT family protein -
  L483_RS30075 (L483_30630) metX 6496610..6497749 (+) 1140 WP_016489640.1 homoserine O-succinyltransferase MetX -
  L483_RS30080 (L483_30635) metW 6497757..6498377 (+) 621 WP_016489641.1 methionine biosynthesis protein MetW -
  L483_RS30085 (L483_30640) - 6498400..6498834 (+) 435 WP_016489642.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 35625.14 Da        Isoelectric Point: 6.7623

>NTDB_id=59163 L483_RS30055 WP_016489636.1 6493316..6494311(-) (pilT) [Pseudomonas putida H8234]
MDVTDLLARAVDAGASDLHLAAGQIPMLRLEGDLQRLDMPALVPADLADGMAALLAERQRRQWAEGDELDLALQLPALGR
FRLNLFRQLNGPAVTFRLIPGRIATVGELDLGDVYQAVAQCTDGLVLVGGPTGSGKSSTLATLLDQLNRDRALHIITLED
PVEVIHSSQRSLINQREIGRHSGGFAQGLRSALRQDPDVIMIGELRDLESVRLALRAAETGHLVLATVHTRSAASSIDRL
VEVFAAEEKPLVRAMLAESLRLVVVQTLVRRVDGGRVAAREVLVATPAVRNLVREGRMAQLCSVMQGGASQGMRTMEGAM
RGLKESGLIRG

Nucleotide


Download         Length: 996 bp        

>NTDB_id=59163 L483_RS30055 WP_016489636.1 6493316..6494311(-) (pilT) [Pseudomonas putida H8234]
ATGGATGTGACCGACCTGTTGGCCCGTGCTGTGGATGCAGGGGCTTCGGACCTGCACCTGGCGGCGGGTCAGATCCCGAT
GCTGCGCCTTGAGGGCGACTTGCAGCGCCTGGACATGCCGGCGTTGGTGCCGGCAGACCTGGCCGACGGCATGGCGGCCT
TGCTGGCTGAACGCCAACGGCGGCAATGGGCAGAGGGCGATGAGTTGGACCTGGCGCTGCAGCTGCCCGCCCTGGGCCGT
TTTCGGCTGAACCTGTTTCGCCAGTTGAACGGCCCTGCGGTTACCTTCCGGTTGATCCCGGGGCGGATCGCCACGGTCGG
TGAACTCGACCTAGGGGATGTGTATCAAGCTGTTGCGCAGTGCACTGATGGCCTGGTCCTGGTAGGTGGGCCTACGGGCA
GTGGCAAGTCCAGCACCCTGGCGACCTTGCTCGACCAACTGAACCGCGACCGGGCGCTGCATATCATCACCCTCGAAGAC
CCCGTCGAAGTTATCCACAGCAGCCAGCGCAGCCTGATCAATCAGCGTGAGATTGGCCGCCATAGCGGCGGGTTCGCCCA
GGGGCTGCGCAGTGCGCTGCGCCAGGACCCTGATGTGATCATGATCGGTGAGCTACGGGACCTGGAATCCGTTCGCCTGG
CCTTGCGCGCCGCCGAGACCGGGCACCTGGTGCTGGCGACCGTGCATACGCGCTCGGCGGCCAGCAGCATTGACCGATTG
GTGGAGGTATTTGCTGCCGAGGAAAAACCGTTGGTGCGGGCAATGCTGGCCGAATCGTTACGCCTCGTGGTGGTGCAGAC
GCTGGTCCGGCGTGTGGACGGTGGGCGGGTGGCGGCGCGGGAAGTGCTGGTGGCTACGCCGGCGGTGCGCAACCTGGTTC
GGGAGGGGCGAATGGCGCAGTTATGTTCGGTGATGCAGGGCGGGGCGTCTCAGGGGATGCGGACGATGGAAGGGGCGATG
CGGGGGCTTAAGGAGAGCGGACTGATCAGAGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L7NL30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.76

99.396

0.505
  pilT Acinetobacter baumannii D1279779

50.456

99.396

0.502
  pilT Acinetobacter nosocomialis M2

50.456

99.396

0.502
  pilT Acinetobacter baumannii strain A118

50.456

99.396

0.502
  pilT Pseudomonas stutzeri DSM 10701

50.152

99.396

0.498
  pilT Acinetobacter baylyi ADP1

49.848

99.396

0.495
  pilT Vibrio cholerae strain A1552

48.632

99.396

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.632

99.396

0.483
  pilT Neisseria meningitidis 8013

47.576

99.698

0.474
  pilT Neisseria gonorrhoeae MS11

47.273

99.698

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.273

99.698

0.471
  pilT Legionella pneumophila strain Lp02

46.809

99.396

0.465
  pilT Legionella pneumophila strain ERS1305867

46.809

99.396

0.465
  pilU Pseudomonas stutzeri DSM 10701

36.97

99.698

0.369

Multiple sequence alignment